FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1186678
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTTAGTACTTGGATGGGAGACCGCCTGGGAATACCGGGTGCTGTAGGCT18930.15952094839543668No Hit
GTCTATAACTGTGATTATTTAGCTTCTCTCTTATTCAGCCTTCTCAGGGT16290.13727396985534407No Hit
GTGTACGGAGCGCGGCTCATGGTTCCAGCTGGCCGGGGGCTCATCGTGGT14830.12497071657180801No Hit
GTTATAGACAACACAGAAGATTCTAGGTGGCACGTTCTTAAAAATCGAGG14290.12042019823406182No Hit
GTCTAGTCTCCAGGTAAATATCATTGCTCCATGCATGCACTTTAGCCATA14250.12008312280163617No Hit
CCGTACACCGAGCACAAGTAGTGGCCTCTGAGTCCAACATTGTTGATATC14200.11966177851110411No Hit
ATCTTACTTTCTGGTACAAATGGAAGATTTGATCCATAAAGAAATTCAAA13860.11679663733548612No Hit
GTCTTAAAGCGTTACCATTAAAAAGGAATTATCCTACTTAGGAAAAAAGG13380.11275173214637838No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG12820.10803267609241934No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)