FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5535278
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGC390750.7059266038670506No Hit
GTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAG293100.5295127001751312No Hit
CCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAATCTG282330.5100556828401391No Hit
GTTCTGTACTGTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACA257010.46431272286595177No Hit
CTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAGGGGAGCAG228600.41298738744467756No Hit
GGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCA227760.41146984848818796No Hit
GTGCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTG220510.3983720420184858No Hit
CTCCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCC215650.3895919951987958No Hit
CTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCC208590.3768374415882996No Hit
AATTAACACTGGCCATTAAGAAGCTGCGGTGAGTAAAACGTCAAGCTCAG205380.37103827486171426No Hit
GTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGG204490.3694304062054336No Hit
GGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAA189230.3418617818292053No Hit
CCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACAC187000.3378330772185245No Hit
GTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATGGG180160.32547597428710895No Hit
GAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACC178960.32330806149212377No Hit
CCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCC177580.3208149617778908No Hit
GTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGC175520.31709337814649957No Hit
GTCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGT166410.3006353068445704No Hit
GCTCTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTA157870.2852069941202592No Hit
GTGTAGGGACATGGCATCAAAGTTCTGGCAAGGAGGGGCGCCTGGGTGGC156520.2827680922259009No Hit
ATCTTGCAGCTCCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTG153210.2767882660997334No Hit
GTGTTAATTTGTGTTATTTCTTAAGCAATTTACTCGATAACGTTTTGTTA150390.2716936710315182No Hit
ACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCT139320.2516946754977799No Hit
CTCCAGGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGA135220.24428764011491383No Hit
GTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCAC132060.2385788030881195No Hit
GGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAAT131590.23772970391008363No Hit
CCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTGCTCTACAGCAC131260.23713352789146272No Hit
CATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAG129170.23335774644019686No Hit
TTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAG127020.22947356934918173No Hit
GTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCA124780.22542679879854274No Hit
CCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGT123460.223042094724059No Hit
CTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGA121350.21923018139287676No Hit
GCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGG119110.21518341084223774No Hit
TTCCAGGAGAGAGCAGGAGGCCTGACAGTTTGCGGGAGCCCTGGAGGAAA118480.2140452566248705No Hit
CACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACT117810.21283483864767044No Hit
GGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGC117280.21187734382988535No Hit
TCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGTT112170.20264564851124012No Hit
GCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGT110520.19966476841813546No Hit
CTATAAAACACGTCAAATGTCAAGTTTATAACAAAACGTTATCGAGTAAA110410.19946604307859514No Hit
CCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGAGCCTCTCC107300.1938475357515919No Hit
GGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGG105310.1902524136999081No Hit
GGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCC105220.19008982024028423No Hit
ACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGA104890.1894936442216633No Hit
CTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCC104340.18850001752396178No Hit
GTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAG104240.18831935812437967No Hit
GTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTG104220.18828322624446325No Hit
ATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGC103710.18736186330659454No Hit
GATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAA102890.18588045623002133No Hit
ACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCC101490.18335122463587195No Hit
GAGCCCAACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTG101060.18257438921766891No Hit
GGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACC101020.1825021254578361No Hit
CTTGTGGGGTGTGTGTGTCAGAATCACATGGGAAACACCTTCAGAGGGTA100880.18224920229842115No Hit
CTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCAGGAGAGGCT98830.1785456846069881No Hit
CCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTG97370.17590805737308948No Hit
GTTTATAACAAAACGTTATCGAGTAAATTGCTTAAGAAATAACACAAATT96860.17498669443522077No Hit
GTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACA96220.17383047427789536No Hit
GCTCTACAGCACGTGGGGACTCTGCCTGTGTGCCCCCACCAGCATTATCA96200.17379434239797892No Hit
GTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACAATTTCACAAA95770.1730175069797759No Hit
GTACTCAGAAGGGGAAGGCAGGAGAGGCTCCTTCTACTGCCAGCCGGAGC94610.17092185794462356No Hit
TTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGG94010.16983790154713096No Hit
GTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTAGTCTTGTGTC92000.16620664761553078No Hit
GCTCTATGGAGGTCACTCACTGTGTGGCACCGGGCCTCATGAAGTTGTAG91390.16510462527807998No Hit
GAATTAAAGAGTCAAGAATCTGAAGGCTTTTCAGACAGAACAGGTCCCTA91270.16488783399858145No Hit
GTGTGGCACCGGGCCTCATGAAGTTGTAGATCCAGCTCCCACCCAGAGAA91100.1645807130192919No Hit
GTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAG89960.16252119586405597No Hit
GCTCATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAG89200.161148184427232No Hit
GCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGT88340.15959451359082596No Hit
CTTCTGTGCAGTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTA87380.15786018335483784No Hit
GACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCC86830.15686655665713628No Hit
GCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTG86780.15677622695734525No Hit
AGCGAAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCA85060.15366888528453312No Hit
GGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGT85030.1536146874646585No Hit
CCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCCCG84400.1524765332472913No Hit
TCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATT83970.15169969782908826No Hit
AAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGG83590.15101319211067626No Hit
TCCCAGGACCCTGGGATCATGACCCAAGCTGAAGGCAGAGGCTTTAAGCC83260.15041701609205535No Hit
TCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACA82490.1490259387152732No Hit
ATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAAT82370.1488091474357747No Hit
CCCCAGATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGAT82230.14855622427635973No Hit
CCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAAT81800.1477793888581567No Hit
GATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCAA81490.14721934471945222No Hit
TCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCA81470.14718321283953578No Hit
CAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCA81420.14709288313974475No Hit
AAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGC80470.1453766188437148No Hit
GGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCAC78620.142034419951446No Hit
AGCAGGAAGTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTC77720.14040848535520709No Hit
GGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATC76950.13901740797842493No Hit
ATCTACAACTTCATGAGGCCCGGTGCCACACAGTGAGTGACCTCCATAGA76800.13874641887905179No Hit
CTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATT75800.1369398248832308No Hit
GTCTTGTGTCTACCTGTGTTCCTTCTTCTTCTTCTTTTTTTAACCTACAT75470.13634364886460987No Hit
GACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCT73740.13321824125183956No Hit
CCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCA73340.13249560365351118No Hit
GCCCAGGCCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACA73240.13231494425392906No Hit
ATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCCCA72670.13128518567631112No Hit
GGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCG72560.1310864603367708No Hit
CTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCA72110.13027349303865135No Hit
GCCTCAGACTCCCCACGCTGAGCCCAGGCCCCTACCCTCTGAAGGTGTTT71750.1296231192001558No Hit
GCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACTCCCCACGCTGAG70930.12814171212358258No Hit
GAACAGGTCCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGA70530.1274190745252542No Hit
CTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAA69940.12635318406771984No Hit
GTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGG69110.1248537110511884No Hit
CTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGC69030.12470918353152272No Hit
GGGCTCTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTT68900.12447432631206598No Hit
GGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTG68260.12331810615474055No Hit
GTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCCAACAT67560.12205349035766587No Hit
GTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGTGT66810.12069854486080013No Hit
CCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGAT66620.12035529200159413No Hit
CCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGA66280.11974105004301501No Hit
GCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAA66110.11943392906372542No Hit
CCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATC65970.1191810059043105No Hit
CTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCCTGTGTCAAG64650.1167963018298268No Hit
CAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTG63980.11558588385262673No Hit
AAAGCAGGGAGCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCT63720.11511616941371328No Hit
ATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATG63370.11448386151517594No Hit
CTTTAATTCAGCCAACATTTCGTGGGTCGCTCTATGGAGGTCACTCACTG63120.11403221301622068No Hit
GTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCCAACATTTCGT61560.11121392638273994No Hit
GAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAAC61290.11072614600386828No Hit
AGCGAAAGCAGGGCCTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTT60730.10971445336620853No Hit
CCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGAG60250.10884728824821445No Hit
GGGGGGAAACTGTCCCCAGATCCCATCCCCTCCCACCAGGTTCCTCCCTT60170.10870276072854877No Hit
TTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAGGGTCTC60030.10844983756913384No Hit
GAGTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGT59610.10769106809088902No Hit
ATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCAC59480.1074562108714323No Hit
GCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCT59430.10736588117164125No Hit
GGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCC59190.10693229861264422No Hit
CCTCCAGAAGGGGGGCTGTGGGGCCTCCAGGATAAGGAGGGGGCGGCTCA57970.1047282539377426No Hit
CTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCC57610.10407788009924705No Hit
CTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCACAAGAACC57440.10377075911995749No Hit
GTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAAT57020.10301198964171267No Hit
AGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCT56850.10270486866242311No Hit
GTGTCTACCTGTGTTCCTTCTTCTTCTTCTTTTTTTAACCTACATTCCTT56720.10247001144296639No Hit
CTTCTCCTCCAGAAGGGGGGCTGTGGGGCCTCCAGGATAAGGAGGGGGCG56280.10167511008480512No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)