FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1339303
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA106370.7942190826123737No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA97490.7279159383649555No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT90250.6738579694064749No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT79010.5899337192554635No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG71620.5347557647522629No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT68550.5118333939369956No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA57360.42828247230089084No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT57060.426042501211451No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG54210.40476277586177284No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA52440.39154694643407806No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA51960.38796299269097434No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT51920.38766432987904903No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG51180.3821390678584309No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA49140.3669072644502402No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC44290.33069439850429666No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT42790.3194945430570976No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC42470.31710524056169515No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA42100.31434260955138604No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC39560.29537752099412906No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA38790.28962826186456686No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC37690.2814150345366209No Hit
GTTTAGCACCAGGTTCCCCACGAACGTGCGTTGCGTGACGGGCGAGGGGG37330.27872706922929313No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA35230.2630472716032145No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT35200.26282327449427056No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG34480.257447343879615No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC33670.25139942193812753No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCG33640.25117542482918354No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA32600.24341019171912553No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT32210.2404982293028538No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC31140.23250899908385184No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA30280.2260877486274577No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT29910.22332511761714863No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA29870.2230264548052233No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC28970.2163065415369039No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT28910.21585854731901596No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC28530.2130212506057255No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT28150.21018395389243508No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG27990.20898930264473384No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG26910.20092540672275055No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT25190.18808290580996234No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC25120.18756024588909306No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG24730.1846482834728213No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA24590.18360296363108272No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT24570.1834536322251201No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG24050.17957101567009107No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA23920.17860036153133385No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC23750.17733104458065127No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA23690.1768830503627633No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA23650.176584387550838No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT23370.17449374786736085No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT23210.17329909661965961No Hit
GGATAAGTAGGCTTCCCACTGAGCGGGGAGCTCCATGCAGTGCTCAATCC23030.17195511396599575No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG22520.16814716311394806No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA22460.1676991688960601No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC22230.1659818577274896No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT21770.16254723539034857No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA20950.15642464774587975No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC20840.15560332501308516No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC20520.1532140225176827No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC20460.15276602829979474No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG20360.15201937126998147No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC20250.15119804853718688No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG20240.15112338283420557No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA20050.14970473447756036No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT19500.14559812081358736No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC19340.14440346956588612No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT19090.14253682699135298No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT19020.14201416707048367No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA19020.14201416707048367No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA19020.14201416707048367No Hit
CCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGGTTCCGGCGGCGT18500.13813155051545467No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA18110.1352195880991829No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA18110.1352195880991829No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC18060.13484625958427632No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT18010.13447293106936967No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA17820.13305428271272446No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG17300.12917166615769546No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG17160.12812634631595687No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT17080.12752902069210625No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA17010.12700636077123698No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT16890.12611037233546105No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT16550.12357173843409594No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG16490.12312374421620798No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT16460.122899747107264No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC16340.12200375867148809No Hit
AGCGAAAGCAGGGCGCCCTGGAATGGGTTCGCCCCGAGAGAGGGGCCCGT16230.12118243593869349No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC16140.12051044461186154No Hit
GGAATGGGTTCGCCCCGAGAGAGGGGCCCGTGCCTTGGAAAGCGTCGCGG15990.11939045906714164No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG15750.1175984821955898No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC15740.11752381649260846No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT15490.1156571739180753No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT15480.11558250821509397No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA15420.115134513997206No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT15270.11401452845248611No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG15070.11252121439285956No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA14990.11192388876900894No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA14630.10923592346168119No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA14540.10856393213484926No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC14410.107593277996092No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA14410.107593277996092No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG14190.10595063253050281No Hit
GGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTT14160.10572663542155883No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG14130.10550263831261483No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCGGGGAG14120.10542797260963352No Hit
GGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGT14050.10490531268876421No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA13850.10341199862913769No Hit
AGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTA13820.10318800152019371No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT13770.10281467300528707No Hit
GCTCTCCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGAC13620.10169468746056716No Hit
TCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAG13590.10147069035162319No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC13570.10132135894566055No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT13500.10079869902479126No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG13490.10072403332180993No Hit
CCCTTATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATT13470.10057470191584728No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)