FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3996957
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA712681.7830564602020988No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG429851.0754431433713196No Hit
GGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTG293650.7346839107851298No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA268970.6729369367746513No Hit
ACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGC266600.6670074258992529No Hit
GCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGATC239340.5988055413155559No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT218780.5473664089956434No Hit
GGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATC202570.5068105561305764No Hit
CTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTTG199810.49990530295922625No Hit
GTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCT190340.47621227849086195No Hit
GTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCA189060.4730098422374822No Hit
AGCGAAAGCAGGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCC170910.42760029692588636No Hit
ATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGG168190.42079511988745444No Hit
CCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTA156690.3920232316734956No Hit
GCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGT154090.38551828303381797No Hit
TTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGAT146760.36717933167657296No Hit
GTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCA145120.36307621022693015No Hit
CCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCC134030.3353301023753821No Hit
GGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTT124890.31246270600359227No Hit
GGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACT123200.30823448938780174No Hit
AGCGAAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGA121760.30463174860274955No Hit
CCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTC119820.29977805615622083No Hit
GTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCA116180.2906711280606722No Hit
ACTGTAAGCTCCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGG114250.285842454647373No Hit
GGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCC113420.2837658748893221No Hit
GCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGC111880.27991294377197456No Hit
TCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTG111800.2797127915061383No Hit
CTGTTACACCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAA110280.2759098984552498No Hit
GCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATGCTAGGGC109110.27298267156739486No Hit
GCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATA107910.2699803875798514No Hit
ACCCAGCAGGGAGCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCAT106390.2661774945289629No Hit
GGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTG106070.26537688546561794No Hit
CTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAG105800.2647013715684207No Hit
AAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTT105700.2644511812361254No Hit
GCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTT103710.25947239362344904No Hit
GCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACA102600.25669528093497124No Hit
TATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGG99280.24838896190276755No Hit
TATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTT99070.24786356220494743No Hit
CATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGAC97170.24310994589133683No Hit
GTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCC96380.241133442266204No Hit
ACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCT96250.24080819483422014No Hit
CCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAG94200.2356792930221666No Hit
TGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGG94100.23542910268987133No Hit
GCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGG93320.23347761809796802No Hit
GAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGA92980.23262697096816404No Hit
CCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGG92340.23102575284147417No Hit
ACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTC90770.22709776462443804No Hit
GGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCA89720.22447076613533748No Hit
CTTTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCA88670.2218437676462369No Hit
CTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCA88340.2210181395496624No Hit
CATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCTG87820.21971714982172685No Hit
TCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGC85560.21406284831185324No Hit
AGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCAT85410.2136875628134103No Hit
CTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAAC85190.21313714408236067No Hit
GCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTT84790.21213638275317948No Hit
GAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCC84470.21133577368983456No Hit
GTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGAT84010.2101848981612762No Hit
GTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAA83370.20858368003458633No Hit
GCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTA82140.2055063389473542No Hit
CCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTG81220.20320458789023751No Hit
GATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCAT80990.20262915012595833No Hit
ATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCG80240.2007527226337436No Hit
AGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGC80110.20042747520175974No Hit
GTCACTGCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACT79270.19832587641047927No Hit
GCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCC79110.19792557187880683No Hit
TCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGAC78420.1961992585859693No Hit
GAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCC77990.1951234401570995No Hit
TATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCA77800.19464807852573845No Hit
CCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCT75220.1881931679525199No Hit
GGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTT75160.1880430537531427No Hit
GCCCAGGGCCTTGGCACTTTAAAAAGCCTCAATAAGCATCGAAAGTATTC74160.18554115043018976No Hit
AAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGC73640.18424016070225424No Hit
GCCTTAGACCTAGGTCATGATCCTGGGGTCCTGGGATTGAGCCCTGCATG73260.18328943743953213No Hit
GTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACA72510.18141300994731743No Hit
GATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGC71430.17871095435852824No Hit
GGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCC71290.17836068789331483No Hit
AGCGAAAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGC69280.17333186221417946No Hit
TCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGT69100.1728815196160479No Hit
GGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCT69010.17265634831698215No Hit
TCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAG69000.1726313292837526No Hit
GAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGC68960.1725312531508345No Hit
ATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCA68800.17213094861916203No Hit
TTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGA68290.17085497792445603No Hit
CAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTC67490.1688534552660937No Hit
GCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATA66980.16757748457138769No Hit
GGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGA66850.1672522371394038No Hit
CTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACA66600.16662676130866555No Hit
ACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATAAAGGTAGGA66270.16580113321209108No Hit
CTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGT66120.16542584771364816No Hit
GAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGAT65320.1634243250552858No Hit
AATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGC64550.16149785949661205No Hit
GGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCAC64370.16104751689848051No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA63840.15972150813731548No Hit
TTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCAAC63580.1590710132733477No Hit
CTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGC63520.15892089907397053No Hit
ATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCG63370.15854561357552757No Hit
GGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCT62850.15724462384759205No Hit
GATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGC62770.15704447158175583No Hit
CGTTAACGTAATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGG62340.15596865315288608No Hit
CCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAG62040.15521808215600016No Hit
GCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAA61790.15459260632526195No Hit
AGCGAAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCA61400.1536168640293103No Hit
TTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGA60580.1515653033044889No Hit
GGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGC60070.1502893326097829No Hit
TCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTT59770.149538761612897No Hit
GTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTT59430.148688114483093No Hit
GCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCC58830.14718697248932125No Hit
GCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATG58740.14696180119025548No Hit
GCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCT57640.14420970753500725No Hit
GCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAG56500.1413575377468409No Hit
GAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCAGGGCTCAAT54960.1375046066294934No Hit
GGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTC54760.13700422596490278No Hit
GACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGG54590.1365789024000008No Hit
GGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCA54520.1364037691673941No Hit
GCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCT53720.13440224650903174No Hit
GCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGG53260.13325137098047338No Hit
TTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGG53210.13312627581432576No Hit
ATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTG53050.13272597128265326No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT52200.13059935345814327No Hit
GGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTC51760.12949851599604398No Hit
TCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAAC51100.12784725980289505No Hit
AAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGA49820.1246448235495153No Hit
TCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACG49470.12376915738648175No Hit
CTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAA49160.12299356735636635No Hit
CAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGT48850.12221797732625095No Hit
GGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGG48220.1206417782327906No Hit
TCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGC47380.11854017944151012No Hit
CTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGA47160.1179897607104605No Hit
GTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCTTGGCACTTTAAAAAGCC47060.11773957037816518No Hit
CAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGA46830.11716413261388603No Hit
CTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGC46820.1171391135806565No Hit
CCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATC46810.11711409454742697No Hit
CCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGA46060.11523766705521227No Hit
CCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTA45470.11376154409467001No Hit
GGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGA45280.11328618246330896No Hit
GGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCT44080.11028389847576545No Hit
CCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTCAAGCCACGGAGG43590.10905796584751849No Hit
CCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCT42990.10755682385374674No Hit
GCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCC42950.10745674772082861No Hit
TTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATG42810.10710648125561521No Hit
GAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGAT42560.10648100542487698No Hit
GTCGTTGGGCGTCTGCCTTAGACCTAGGTCATGATCCTGGGGTCCTGGGA42100.10533012989631862No Hit
GAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCA41510.1038540069357764No Hit
AAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTC41370.10350374047056299No Hit
ATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCT41240.1031784930385791No Hit
ATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATCAG40340.10092678004792145No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)