FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences986143
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAG132141.339967935684784No Hit
CTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCT131701.335506108140503No Hit
GAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCC113901.1550049029400402No Hit
GACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGA93910.9522959651896328No Hit
CTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATG77200.7828479236784117No Hit
CTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCT68130.6908734331633445No Hit
ATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTC59090.5992031581626599No Hit
GGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGA56020.5680717705241531No Hit
TCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCT54650.5541792620340052No Hit
CTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCC49550.5024626245889288No Hit
CCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTC49420.5011443573599367No Hit
CTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCT48130.48806309024147615No Hit
AAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAG47600.4826886161540467No Hit
AAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCT46170.468187676635133No Hit
GAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAA44130.4475010216571025No Hit
AGCGAAAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGG40170.4073445737585725No Hit
GAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAAT38740.39284363423965896No Hit
ACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCA38090.38625229809469824No Hit
AACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATC37840.38371716880817486No Hit
TAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGA37750.3828045222650264No Hit
CTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACT36740.3725625999474721No Hit
GGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAG35980.36485580691644115No Hit
GACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGC34100.3457916346817855No Hit
CTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTT33970.3444733674527934No Hit
GAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCC33690.3416340226518872No Hit
CCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTA32570.3302766434482626No Hit
CCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGA30020.30441832472572433No Hit
CCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCC28840.29245251449333415No Hit
CCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTAC27240.27622768705958467No Hit
CTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGGTATAT27200.2758220663737409No Hit
GTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGG26880.27257710088699105No Hit
GTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAG26060.26426187682719443No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA23870.24205414427724983No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22890.2321164374740783No Hit
ATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAG22770.2308995754165471No Hit
TCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTT22750.23069676507362522No Hit
CTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCAC22640.22958130818755496No Hit
CCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCA21640.21944079104146155No Hit
CCCCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTG20060.20341877395063393No Hit
CTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTG19960.20240472223602457No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT19920.20199910155018086No Hit
GGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATTCA18860.19125015337532184No Hit
AGCGAAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCC18610.18871502408879848No Hit
GGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCAGAGAGAG18360.18617989480227512No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT17710.1795885586573144No Hit
GGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTA17320.17563375697033798No Hit
TACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAA16800.1703606880543694No Hit
GGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCT16730.16965085185414286No Hit
TAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGG16730.16965085185414286No Hit
GAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAG16560.16792696393930698No Hit
CTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCC16410.16640588636739295No Hit
GGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACC16320.16549323982424455No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT16080.16305951570918215No Hit
ACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGG15700.15920611919366662No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT15290.15504850716376833No Hit
CCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCC15130.15342602442039338No Hit
GGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGA14820.15028246410510443No Hit
AATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTT14790.14997824859072162No Hit
CATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTG14120.14318410210283905No Hit
GGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAG14100.14298129175991717No Hit
ATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCC13900.1409531883306985No Hit
CCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTC13820.14014194695901103No Hit
CCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCT13770.13963492110170636No Hit
TAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTC13650.13841805904417512No Hit
CCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCC13540.13730260215810486No Hit
AAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATT13410.13598433492911272No Hit
GGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTC13030.13213093841359722No Hit
GTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCAC12680.12858175741246453No Hit
CTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCT12560.1273648953549333No Hit
AGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCC12200.12371430918233968No Hit
AGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGA12090.1225988522962694No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11870.12036793852412886No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT11510.11671735235153523No Hit
CAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTAT11390.11550049029400401No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA11300.1145878437508556No Hit
ACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTC11300.1145878437508556No Hit
CTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTC11270.11428362823647281No Hit
GTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTC11210.11367519720770719No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC10840.10992320586365263No Hit
CATTAATAGAGGTGGAGCGTGCAGAGAGAGGGAGGTGACGGTCCCAGTCT10460.10606980934813715No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC10280.10424451626184032No Hit
AAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGA10250.10394030074745751No Hit
CCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCA10200.10343327489015285No Hit
AATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAA10160.10302765420430911No Hit
ATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTT10000.10140517146093417No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)