FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A11a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n02_CIVIR_Ferret_Flu_R2_A11a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences752680
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAATG41720.5542860179624808No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT28380.37705266514322155No Hit
ATCTAAGACTGTCTCTCTTTTAATCACACACACACATGCACACACACAGA27560.3661582611468353No Hit
ATATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGA25170.334405059254929No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT23810.3163362916511665No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA17410.23130679704522505No Hit
GTCTTAGATTCTCCTTCATGAGTATGTCCCTTAGAGGGATAAATGATGAA15810.2100494233937397No Hit
GTCCATGAGCATGTCTGGTTTTACAACTCTTGTCAGAGGCTCAGACACTC15420.20486793856619015No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG15000.19928787798267522No Hit
CAATAATGGTCAACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACA14490.19251209013126427No Hit
ACTTAGGACTGGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAAT14070.18693202954774937No Hit
GTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCC13410.17816336291651166No Hit
ACAGGAGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACT13170.17497475686878886No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC13020.17298187808896212No Hit
GTAGGAGACAGAGTGGCATTTTGTACAATATTAAATTCAGGTTCTCAATA13020.17298187808896212No Hit
ACCTACAACAGTCAAAACACTGCTTACAACTTAGGACTGGGCTATACATC12980.17245044374767496No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC12610.16753467609076897No Hit
GTTCTGGAGACTGAATAGGAAAACCAGAAAAGAGAACATATCAGGCAGAG12610.16753467609076897No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT12560.16687038316416009No Hit
CATATATACTTACACAGTTTGAGCATTCACAGGCCACTAGAAGCCCATCT12520.16633894882287292No Hit
TTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTGCTCCT12280.16315034277515014No Hit
ATTAAATTCAGGTTCTCAATAATGGTCAACAGGAGATTGTACCCATGAAA12100.16075888823935805No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT11790.15664027209438275No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG11610.15424881755859063No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11570.15371738321730352No Hit
CGATTGGACCAGGCGATCATGGAAAAGAACATAGTACTGAAAGCGAACTT11560.15358452463198172No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA11450.1521230801934421No Hit
CTCTACGGAAAGCTTACATTGCCTCTTGTGAGTGTATCCAATCTCTAGAA11180.14853589838975395No Hit
CTTTCAATCTGTGCCGCATTTCTTCAATTAACCACCTTATTTCCTCAAAT11150.1481373226337886No Hit
ATCTTAAGGTTAGCTGATTGACAACCTTAACTCCATCTTTGACCTAATAC11150.1481373226337886No Hit
AAACTATTCTCTGTCGCTTTCAATCTGTGCCGCATTTCTTCAATTAACCA11110.14760588829250146No Hit
ATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTCCT10930.14521443375670937No Hit
GAATAATGTCCCTCCCCAAAAATGATAGCCCAATTCCTAGAAACTGTGAA10830.14388584790349151No Hit
ATCTTTTATTGCTTATTTGGTCACCACTTCCTCTCTGCCTGATATGTTCT10810.14362013073284796No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC10760.14295583780623905No Hit
GTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGGCACACCCCAAGT10730.1425572620502737No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA10660.14162725195302123No Hit
CATCTGGATGTATAAAAAAATGCTCATGATCTCAACAGAGGAATGCTCTT10610.1409629590264123No Hit
CTCTAAGGGACATACTCATGAAGGAGAATCTAAGACTGTCTCTCTTTTAA10570.14043152468512515No Hit
TTGTACACCTGTGTTCATAGGAGCATTATTCACAATAACCAAAAGGTGGG10330.13724291863740234No Hit
AGTTAGGGTTGCTTTGGGCTCCCAGATCACCAACAGATTTCATCATTTAT10110.1343200297603231No Hit
ATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTTCAGTA9970.13246000956581816No Hit
GTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATAGAATGATGGTTA9820.1304671307859914No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT9760.1296699792740607No Hit
CAATAGAGAATGGGCTTACCATGATCCGATTAAACAAACATGACTTACTC9730.12927140351809532No Hit
GTCTAGGGAATATCCTCTAAGGAGTAAGTCATGTTTGTTTAATCGGATCA9700.12887282776213No Hit
CTTAATGACTGAGCCACTCAAGTGCCAACTGTGGCATACCTGTGAGAGCA9590.12741138332359037No Hit
GTCCTGGGGAGACACCAAACTGTGTTTTAGTGAGCTTCCTAAGGCTTGAA9570.12714566615294678No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC9410.12501992878779827No Hit
GAGTGATACAATCCCACCTGCGTGTTAGAAAGGATTTTCAAGTGAAGGTT9390.12475421161715469No Hit
CTCTAGAACTTTCTCATCTTGCAAAACTGAAATTCTGTAACTCCCCATGA9360.12435563586118935No Hit
GCTCTGGAGGTAGTGAAGGTCTCCCATTCTCATCACAGTTTCTCCAAGCG9350.12422277727586757No Hit
GAACTATCCTGATTGATCCTCTAAGCAGACGAGAGGTTACCTGAGGTTTT9310.12369134293458044No Hit
CTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTT9220.12249561566668439No Hit
CTTCTACCCTGTAAGTATCTCTCCGTTCATTGCTGTTAACTGTCCCCCAG9050.12023701971621407No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG9040.12010416113089228No Hit
ACTTAACACAAGGTGCCTAGAGTCCTCAAGCTCATAGAGACAGAGAAATA8910.11837699952170909No Hit
GAACAATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAG8830.11731413083913482No Hit
CATATGAACAAGTACTGAATTTTGTCTATGCTCAAATGAGGTGATGTGTG8800.11691555508316948No Hit
TGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATT8720.1158526864005952No Hit
GCTCTTATCTCTTGTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAA8610.11439124196205559No Hit
GGGCTATACATCCAACAGCAGCAGCACTATGGGTCAAAATAATGCAGTTG8610.11439124196205559No Hit
GAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGA8540.1134612318648031No Hit
CATCAGGAAAGACTACTTTGGGGAGAAAGAACAATAATCACCTGATGTAA8470.11253122176755062No Hit
CTCCCATTCTCATCACAGTTTCTCCAAGCGAATCTCTGTATATTTTCAGA8410.11173407025561993No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT8360.11106977732901101No Hit
GGTGATTATTGTTCTTTCTCCCCAAAGTAGTCTTTCCTGATGTCTGACTG8330.11067120157304566No Hit
ATAATCACCTGATGTAAAAATCCAGTGACAACACTGACAAGCAAGTTGAT8190.10881118137854068No Hit
AGACTATACTCTAAGTGGTATTAGGTCAAAGATGGAGTTAAGGTTGTCAA8150.10827974703725356No Hit
AAGTAAGATATGCCAGTTGCGAAAAGAAATACTTTGTGATTCTACTTAAC8140.10814688845193178No Hit
ATGTATATACAGCTCGCTGAAGAGGTCCATGACCTCAAAAAAGATCAAAT8130.10801402986660998No Hit
GCCTGATATGTTCTCTTTTCTGGTTTTCCTATTCAGTCTCCAGAACCCAT8040.10681830259871392No Hit
GGTGTACATAGAATGCTCCACAAATATTGGCTGGAAGGAAATGGTAGCCT7980.10602115108678323No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA7910.10509114098953075No Hit
GTGTAATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACT7780.10336397938034757No Hit
GGGTAGAGGTGGGGAAGGCAGAGATCTGAGAACTCTTTAGGAAGCAGAAT7760.10309826220970399No Hit
ATATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGG7750.10296540362438221No Hit
CTTCAACTCAGTAATTGAGTTATCTTTTATTGCTTATTTGGTCACCACTT7730.10269968645373864No Hit
CTCCAGAACCCATCCACATGCCGGTTCTCTGAGTTCCAGAATTTCCCCAG7680.10203539352712972No Hit
CCTTATTTCCTCAAATTTCTGTCCCAATTGCTCTCGCCACTTTTCATTTC7670.10190253494180793No Hit
AGATTGTACCCATGAAAAAGGAGCAGTCAGACATCAGGAAAGACTACTTT7620.10123824201519903No Hit
TATATAGACTCTCTGTGGACTTCAGAGTCAAAACCAGCTATTAACTCGGC7610.10110538342987725No Hit
GTATCAAGGTCTCTAATCGGTTAAAGATTACACTGAAGTTCGCTTTCAGT7570.1005739490885901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)