FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_undetermined.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_undetermined.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84058984
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT9862791.1733177741001486No Hit
GCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAG6389730.7601483739084927No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC5075600.6038141027257717No Hit
CCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAAC3998440.47567075043400475No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC3684290.4382981835707175No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA3330100.39616229480004184No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC2787540.33161714160142597No Hit
TTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAATTCTTTTATTC2737850.325705816287287No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA2693620.32044403486961015No Hit
ATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATATAGCAGAACTAC2668870.3174996738004828No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA1983150.23592362239353262No Hit
TCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTC1877050.2233015331234553No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA1781570.21194284242122174No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA1697370.20192606658200868No Hit
GAATAAAAGAATTCGGATGGCCATCAATTAGTTGTCGAATAGTTTAAAAA1686580.20064244411995272No Hit
GTATATAGCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTTTATTTT1644930.1956875900379667No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT1617180.19238633671803598No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT1545480.18385661192383673No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG1515660.18030910295085176No Hit
GTAGAAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATC1474080.17536257635471778No Hit
ATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATT1319170.15693385016407052No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC1283220.15265709135861077No Hit
AATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATG1277200.1519409275753321No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG1221760.1453455587804868No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG1151160.13694669447824875No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG1126990.13407133257761003No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCC1124190.13373823314352692No Hit
GAGCAAAAGCAGGTCAATTATATTCAATATGGAGAGAATAAAAGAATTCG1112020.13229044024610148No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAA1055300.12554279742424676No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGCTATATACATTTGCAACCGC1028430.12234623249788505No Hit
AAACAAGGTCGTTTTTAAACTATTCGACAACTAATTGATGGCCATCCGAA1021660.12154084565190557No Hit
TAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGT969700.11535947186799213No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATT963840.11466234233808964No Hit
GTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGG931790.11084954345867422No Hit
CATGATAACCTATACATAATGTGTCTGCATTTGCGGTTGCAAATGTATAT917920.10919951161912687No Hit
TTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGT879260.10460036014710812No Hit
GTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACAGTGTCTGTTGAA862710.10263150456350985No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA854650.10167265404968492No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)