FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4913510
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAAT347980.708210627433342No Hit
GTTCACAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACT296260.6029498260917348No Hit
CCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGG237820.4840124473136312No Hit
CAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTT236720.4817737218403952No Hit
CCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATC201010.4090965521592507No Hit
ACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAG175100.3563643912396637No Hit
CTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGG173140.3523753894873522No Hit
GGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCAC154970.31539571507944425No Hit
ATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACC153830.3130755814071814No Hit
TCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGA153420.3122411473671571No Hit
CTCCAGGATTCCCACTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAG146140.29742485514428585No Hit
CCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCC145350.29581704321350727No Hit
GCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGGGACCT145200.2955117624671569No Hit
GCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAG138650.2821811698765241No Hit
CCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGG135860.2765029479944073No Hit
GCATAGCCCTGGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGT132480.2696239551766456No Hit
AATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAAC127880.26026201228856766No Hit
GTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTCACCA127620.2597328589948937No Hit
CTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCT118670.24151777446265502No Hit
GCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGA118570.2413142539650881No Hit
CATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATCACCCCACAG117970.24009313097968663No Hit
GGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTAT114550.2331327299628982No Hit
AGTAGAAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGA114320.2326646328184943No Hit
CTCCCACCTCTCCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCAC113070.2301206265989079No Hit
CACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCT112910.22979499380280086No Hit
GACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTCATTCCA111720.22737309988175458No Hit
TGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAG108680.22118607675572044No Hit
TCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTTGAACATGGATGAAT108110.22002600991958907No Hit
GGATTGGGAACCCAGCTTTTTAGTTCAATTCACCAAATGTTTGTTGTCAC107770.21933404022786157No Hit
CCAGGGGACTTGGCTGCAAACCCAACAGATGATGGAGAAGACATATGCCT104530.2127399761066936No Hit
TTTTAGTTCAATTCACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGT102170.20793689236411445No Hit
GACATATGCCTTCCGGCATATTCTCGTCTCCAGCACACAAATTCATTCCA101280.20612555993576895No Hit
TCTTCAGGCTGGGAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAA99030.2015463487405134No Hit
CTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATTC97810.1990633986701971No Hit
GGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCC96140.19566460636082963No Hit
GGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGATGATGGA95150.1936497534349172No Hit
CTTTTACTCCCAGCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGC94690.1927135591461094No Hit
AATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGAG88920.18097042643649855No Hit
GCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGG87860.17881310916228926No Hit
GAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCA87760.17860958866472237No Hit
GGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTGAGAG87630.17834501201788538No Hit
GATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGAGATGGTG86730.17651332753978316No Hit
GAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTT86210.17545502095243523No Hit
CACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTG85940.17490551560900455No Hit
CAGCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAA85780.1745798828128975No Hit
AGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCAT85640.1742949541163038No Hit
GGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCTGGTCTCACCA83840.1706315851600994No Hit
GTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCACTGTGGCATC83030.16898306912980743No Hit
CACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACC82150.16719208875121858No Hit
AGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCT81850.16658152725851783No Hit
CACAAATTCATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAA81040.16493301122822585No Hit
GGTTTCATTCATCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGA80950.16474984278041563No Hit
CTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAATA80760.1643631538350385No Hit
CCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAG80380.16358977594428423No Hit
GCATATGTCTTCTCCATCATCTGTTGGGTTTGCAGCCAAGTCCCCTGGAG79730.1622668927100993No Hit
GCCTGAAGATCTGCATGAGAACCAATGCACACCAGGGCTATGCTGCCTCC79300.16139175457056157No Hit
GCATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGG77180.15707712002214302No Hit
TCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGAT77110.15693465567384618No Hit
GAGTAAAAGTCGGTCACTGCCCTGCTGCTGAGTGGGAATCCTGGAGTGCT74890.15241650062786072No Hit
GTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTG73880.1503609436024349No Hit
GCACATGCTGAGACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAG73520.14962826981119404No Hit
CCCGGGCACAGAGTAGTTGTTGAACATGGATGAATGAAACCTGTCGAGTG73260.14909911651752006No Hit
AGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAA72510.14757271278576822No Hit
CCCCACCACCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGG72480.14751165663649815No Hit
CTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCA72470.14749130458674145No Hit
GACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGT71820.14616842135255653No Hit
GAGGTGGGAGAGGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCA71450.14541539551155894No Hit
CATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGG71150.14480483401885821No Hit
ATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTTTC70840.1441739204764008No Hit
CTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACA70540.14356335898370004No Hit
ACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACG70070.14260681264513556No Hit
GGAGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATC69820.14209801140121828No Hit
GTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACA69550.1415485060577876No Hit
CCCTCCTAGCGCAGCACTCCAGGATTCCCACTCAGCAGCAGGGCAGTGAC68840.14010351052506254No Hit
ATCCTGGAGTGCTGCGCTAGGAGGGTGGTGGGGAGAGGTGGGAGAGGGCA67740.1378647850518265No Hit
AGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGGGACCAC66830.1360127485239676No Hit
GCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCA66690.13572781982737392No Hit
GCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTTCA66130.13458810504099922No Hit
ACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTTTTAGTTCAATT65530.13336698205559774No Hit
CTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGCAGTATCTG65520.13334663000584104No Hit
GTATGGATGCCTCCCCACTCCCCGAGTCTCAGCATGTGCTGGGCACAGCC65020.13232902751800646No Hit
ATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATGGATGCCTCCCCACT64140.13053804713941763No Hit
CTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAG63860.12996818974623028No Hit
GCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGTGTGGG63480.12919481185547602No Hit
ACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCTACTAAT63320.12886917905936898No Hit
GTGCAGCTGTGAACAGTGGGCTCCACCTGACACCCACACCCTCAGGGAGG62890.12799404091983124No Hit
CTGGAGAGATGAGGAATCCTGGACGGGAGCAAGTCTGTGGTGGAAGCGGA62650.12750559172567066No Hit
GACTCGGGGAGTGGGGAGGCATCCATACTTTGGAGAAAGAGCAGAGCCCA62380.12695608638223999No Hit
AGCGAAAGCAGGCTAGGAACCTAGGTCTCTGCACTCCAGAGCATGGGCTG62200.12658974948661955No Hit
CATACATGCAGCCTCAGTTTTGGTGATGTTATTTTTCAAGCTGGGTGGCA61560.1252872183021913No Hit
ACCCCACAGATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTC61400.12496158550608424No Hit
GTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCTTT61380.12492088140657086No Hit
GTATCTGTGGGGTGATGCCACAGTGGGCTCTGCTCTTTCTCCAAAGTATG60780.12369975842116938No Hit
ATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGGT60370.12286532438114506No Hit
GATACTGCTGGGAGCCGAGGGAAGGCAATGCTGAGGTCCCTCCCTGAGGG59060.1201992058630185No Hit
CTCAGCAGCAGGGCAGTGACCGACTTTTACTCCCAGCCTGAAGATCTGCA59010.12009744561423503No Hit
ATGCTGAGGTCCCTCCCTGAGGGTGTGGGTGTCAGGTGGAGCCCACTGTT58990.12005674151472165No Hit
GTTTTGAGCTTGGGAGACTGGAGAGATGAGGAATCCTGGACGGGAGCAAG58900.11987357306691145No Hit
GCATGGGCTGTGCCCAGCACATGCTGAGACTCGGGGAGTGGGGAGGCATC58760.11958864437031777No Hit
GTTGAACATGGATGAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGT58380.11881526647956347No Hit
GAGCAGAGCCCACTGTGGCATCACCCCACAGATACTGCTGGGAGCCGAGG57590.11720745454878488No Hit
GGGCAGAGGGCAGACTGAGCTCCAGGGGACTTGGCTGCAAACCCAACAGA57590.11720745454878488No Hit
ATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCAGCT57490.11700393405121798No Hit
GGTGTGCATTGGTTCTCATGCAGATCTTCAGGCTGGGAGTAAAAGTCGGT57190.11639337255851723No Hit
CTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGCTGGGTTCCCAAT56770.1155385864687362No Hit
CCCACACCCTCAGGGAGGGACCTCAGCATTGCCTTCCCTCGGCTCCCAGC56580.11515189752335908No Hit
CCCCAGGGCCTGGTCTCACCACCATCTCCGCTTCCACCACAGACTTGCTC56390.11476520857798193No Hit
GCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCTGGTGTGCATTG55790.11354408559258046No Hit
GAACCTAGGTCTCTGCACTCCAGAGCATGGGCTGTGCCCAGCACATGCTG55120.11218049825888214No Hit
GAATGAAACCTGTCGAGTGAGAGTGGAATGAATTTGTGTGCTGGAGACGA54910.11175310521399162No Hit
TCCATGTTCAACAACTACTCTGTGCCCGGGCAACCAGGATTGGGAACCCA54530.11097972732323737No Hit
TCTGTTGGGTTTGCAGCCAAGTCCCCTGGAGCTCAGTCTGCCCTCTGCCC54020.1099417727856461No Hit
GGGTGTGGGTGTCAGGTGGAGCCCACTGTTCACAGCTGCACAGGCTCTGT53940.10977895638759258No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCC53880.10965684408905242No Hit
CACCAAATGTTTGTTGTCACAGAGCCTGTGCAGCTGTGAACAGTGGGCTC53750.10939226744221545No Hit
GGAGCAAGTCTGTGGTGGAAGCGGAGATGGTGGTGAGACCAGGCCCTGGG53190.10825255265584073No Hit
ATGCACACCAGGGCTATGCTGCCTCCGTGACCAAGTGGTCCCCAGGGCCT52960.10778445551143684No Hit
GGTGAGACCAGGCCCTGGGGACCACTTGGTCACGGAGGCAGCATAGCCCT52350.10654298047627866No Hit
GCTGCACAGGCTCTGTGACAACAAACATTTGGTGAATTGAACTAAAAAGC51280.10436531115231269No Hit
GGAGAAGACATATGCCTTCCAGCATATTCTCGTCTCCAGCACACAAATTC50870.10353087711228837No Hit
ATTCCACTCTCACTCGACAGGTTTCATTCATCCATGTTCAACAACTACTC50790.10336806071423482No Hit
GTTTCTACTAATAACCCGCCTTGTTTCTACTAATAACCCGCCTTGTTTCT50740.10326630046545138No Hit
AAGCAGGAGATCACCCTGGGGTTTTGAGCTTGGGAGACTGGAGAGATGAG50580.10294066766934432No Hit
CCCAACAGATGATGGAGAAGACATATGCCTTCCGGCATATTCTCGTCTCC50480.1027371471717774No Hit
CTAAAAAGCTGGGTTCCCAATCCTGGTTGCCCGGGCACAGAGTAGTTGTT50430.10263538692299395No Hit
ATATTCTCGTCTCCAGCACACAAATTCATTCCACTCTCACTCGACAGGTT50410.10259468282348057No Hit
AAACAAGGCGGGTTATTAGTAGAAACAAGGCGGGTTATTAGTAGAAACAA50100.10196376928102313No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)