FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1027447
Sequences flagged as poor quality0
Sequence length151
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTTAAAGCGCGCGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATT79140.7702587092083581No Hit
ACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGC53720.5228493537866187No Hit
TGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGCACAGC52040.5064981454031205No Hit
ACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCAC44230.4304844921441203No Hit
GAACTATATTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCC41630.40517905059823034No Hit
ATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGCCAG39440.3838640825268846No Hit
ACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCG38340.37315793418054655No Hit
CCATTTACGGCGATGACCCCTTGGATGCACAGCTGGCAGTCATGGCGATG38070.3705300614046272No Hit
CTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCACCGCTTGTAGTAG37460.3645930155034761No Hit
ATTTACGGCGATGACCCCTTGGATGCACAGCTGGCAGTCATGGCGATGGG32170.3131061748197231No Hit
CCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAA31010.30181605474540296No Hit
CTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCG30850.3002587968041174No Hit
GCCTACTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAA30290.294808394009618No Hit
GTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTCAA29010.2823503304793337No Hit
ATATTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCCCTCGA27700.2696002810850584No Hit
GGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCA27620.2688216521144156No Hit
CTATATTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCCCTC27330.26599912209583565No Hit
GCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTCGGCAAGCGCGGTGGTCAG27030.26307926345592525No Hit
CGCCTAGCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGC25650.24964791371233747No Hit
TCCAAGGGGTCATCGCCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGC25480.24799332714972158No Hit
GTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCT25470.24789599852839123No Hit
GCTTTAGCTCGCGGTGCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGG25380.24702004093641816No Hit
CCCCTTGGATGCACAGCTGGCAGTCATGGCGATGGGCAAGTGCGGCGCAG24070.2342699915421428No Hit
GAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGACTGC23820.23183677600888417No Hit
ATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCGAGTCT23520.22891691736897377No Hit
GTTTAATCGCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGC23200.2258024014864027No Hit
GTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAGGAGCG23110.2249264438944296No Hit
TCCCTTGCCTGCGCCGTGCGCACCATTTACGGCGATGACCCCTTGGATGC22650.22044932731323366No Hit
CCGTAAATGGTGCGCACGGCGCAGGCAAGGGAGGCGGTTAAGGCGGCGTC22300.21704282556667154No Hit
GCGTCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAAT22270.21675083970268053No Hit
CATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCGATA22150.2155828962467164No Hit
ATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTAGCTCGCGGTGCTTG22150.2155828962467164No Hit
CGCCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCA21470.20896454999625286No Hit
GTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGG20910.20351414720175348No Hit
AAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCTTTAAG20850.2029301754737714No Hit
TCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTG20390.1984530588925755No Hit
CGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTAC19980.1944625854180313No Hit
GAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCACGCCTAGCTTTA19800.19271067023408506No Hit
TCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAG19340.18823355365288916No Hit
CCTGTACATCCTCAACAAAAGACTCGCGCTGCGATGCCGTCCACACCATC19170.18657896709027327No Hit
GTCCATGGTCGCTCTGATGAGACGCTGCGCGCGCTTTCCACGGTGGAGGC18450.17957130635448834No Hit
ACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTG18230.17743007668522076No Hit
GGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGA18070.1758728187439352No Hit
GTCATAGCGCGCGCTTTAAGGAGCGCCTGAAATTCCCACGTCTCCGCCCA17990.17509418977329244No Hit
GTCATGGCGATGGGCAAGTGCGGCGCAGGCGAGCTGAACTATATTTCCGA17720.1724663169973731No Hit
GGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCATCGCAGCGCG17700.1722716597547124No Hit
AACTCATAGGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCC17660.17188234526939103No Hit
GCGCACGACCTACCGCACTTTGATGATGCGCATTGCCGCCTGCGATGTGG16960.16506934177626678No Hit
CGCTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTC16880.16429071280562405No Hit
CTTTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCA16810.1636094124563116No Hit
GCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAATCAGCGCCTCC16750.16302544072832956No Hit
CTACAAGCGGTGGGCGGAGACGTGGGAATTTCAGGCGCTCCTTAAAGCGC16710.16263612624300816No Hit
ATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAGCTC16640.16195482589369573No Hit
AGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAGCTCTTG16610.16166284002970469No Hit
CTATGACGGGCTACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGC16600.16156551140837436No Hit
GCTGGCAGTCATGGCGATGGGCAAGTGCGGCGCAGGCGAGCTGAACTATA16550.16107886830172263No Hit
GTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGCCTAGTGGGAG15990.15562846550722323No Hit
GACGTACCCCGCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCG15970.15543380826456255No Hit
CTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCA15770.15348723583795562No Hit
ATATCGAATTTGCGGTCCAGCTCTTGCAATTGGTCCATGGTCGCTCTGAT15740.1531952499739646No Hit
GGTGCGCACCTTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGA15490.15076203444070593No Hit
ATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGGTGCGCACCTTG15420.15008073409139353No Hit
CACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGGACGGCA15410.1499834054700632No Hit
ACCTCCCACTAGGCCAGGACTATTTGGACCAGATTCGCCCGATGGTGTGG15220.1481341616647866No Hit
GCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCG15050.14647957510217072No Hit
CCTTCCATTCACGTAAGGGCCAGACCAAGCTGCAGCCGGAGGTAGGATTT14990.14589560337418864No Hit
GTAGTAGGCCACGTGGGATTCCAAGGTGCGCACCAATGCGCCCGACTTTC14870.1447276599182245No Hit
AAACAAGGAAGTGATGGGACCGCAGAGATGAGACCCCCAGGGATGAGATG14600.14209978714230515No Hit
GGCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAA13950.13577342675583265No Hit
GCCTTAACCGCCTCCCTTGCCTGCGCCGTGCGCACCATTTACGGCGATGA13830.13460548329986852No Hit
TGGATGCACAGCTGGCAGTCATGGCGATGGGCAAGTGCGGCGCAGGCGAG13660.13295089673725263No Hit
GGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAA13640.13275623949459192No Hit
GGCAAGGGAGGCGGTTAAGGCGGCGTCGGCAAGCGCGGTGGTCAGCGCCG13610.13246425363060088No Hit
AGCTAAAGCTAGGCGTGGGCGGCCTGCGCGATATCGAATTTGCGGTCCAG13470.13110165293197604No Hit
GTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGAT13330.1297390522333512No Hit
TTTCCGATGTGGACGTCATTTTCGTCGGCAGCGAGGCAACCCCTCGAGCC12950.12604056462279806No Hit
TCTCATCAGAGCGACCATGGACCAATTGCAAGAGCTGGACCGCAAATTCG12670.12331536322554837No Hit
CCCTCGAGCCACGCGGCTGGCGGCGGAGTTTAATCGCATTGGATCCACCA12660.12321803460421801No Hit
ATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGT12590.12253673425490559No Hit
ATGGTGTGGACGGCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGC12370.120395504585638No Hit
AGCGAAAGCAGGCGCAGAAACTCATAGGCTTCAATCAGCTGGGTGCCGTC12360.12029817596430764No Hit
GCATCGCAGCGCGAGTCTTTTGTTGAGGATGTACAGGCGATGCGCCGCCG12350.12020084734297731No Hit
CGCCTCCACCGTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGA12260.11932488975100418No Hit
GTGCGGCGCAGGCGAGCTGAACTATATTTCCGATGTGGACGTCATTTTCG12080.11757297456705797No Hit
CATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAGG12050.11728098870306693No Hit
GGTCCAAATAGTCCTGGCCTAGTGGGAGGTAGCCCGTCATAGCGCGCGCT12010.11689167421774554No Hit
TCGGAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATGAC11840.11523708765512966No Hit
GTTCCCGCAGAGCTCAAGCACCGCGAGCTAAAGCTAGGCGTGGGCGGCCT11820.11504243041246895No Hit
GCATTGGATCCACCACCTTCTTCGAGGTGGATGCGAACCTGCGCCCCGAG11660.11348517247118342No Hit
GCTGAAATCAGCGCCTCCAGCGCCTCCACCGTGGAAAGCGCGCGCAGCGT11640.11329051522852274No Hit
GCTTCAATCAGCTGGGTGCCGTCCTCGCGGCCGACGTACCCCGCTGAAAT11580.11270654350054067No Hit
ACATTGGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGA11450.11144127142324615No Hit
GTGGAAAGCGCGCGCAGCGTCTCATCAGAGCGACCATGGACCAATTGCAA11310.11007867072462132No Hit
TTGATGATGCGCATTGCCGCCTGCGATGTGGCGGGCACCTTCCATTCACG11300.10998134210329097No Hit
ACAAAAGACTCGCGCTGCGATGCCGTCCACACCATCGGGCGAATCTGGTC11290.10988401348196063No Hit
CTGCGATGCCGTCCACACCATCGGGCGAATCTGGTCCAAATAGTCCTGGC11170.10871607002599648No Hit
GACCAATTGCAAGAGCTGGACCGCAAATTCGATATCGCGCAGGCCGCCCA11130.1083267555406751No Hit
GGCCAGCACCCGGCGGCGCATCGCCTGTACATCCTCAACAAAAGACTCGC11000.1070614834633806No Hit
TTGGAATCCCACGTGGCCTACTACAAGCGGTGGGCGGAGACGTGGGAATT10920.10628285449273782No Hit
CTTCGAGGTGGATGCGAACCTGCGCCCCGAGGGAAAGTCGGGCGCATTGG10850.1056015541434254No Hit
CGCCATGACTGCCAGCTGTGCATCCAAGGGGTCATCGCCGTAAATGGTGC10800.10511491103677367No Hit
GGCCAGACCAAGCTGCAGCCGGAGGTAGGATTTCGCCAGATTACGGCGCT10750.10462826793012193No Hit
GCGAAAGCAGGCGCAGAAACTCATAGGCTTCAATCAGCTGGGTGCCGTCC10670.10384963895947918No Hit
GCTTGAGCTCTGCGGGAACATTGGCCAGCACCCGGCGGCGCATCGCCTGT10660.10375231033814883No Hit
ACTGCCAGCTGTGCATCCAAGGGGTCATCGCCGTAAATGGTGCGCACGGC10600.10316833861016676No Hit
AGGATGTACAGGCGATGCGCCGCCGGGTGCTGGCCAATGTTCCCGCAGAG10370.10092978031956881No Hit
CATCGGAAATATAGTTCAGCTCGCCTGCGCCGCACTTGCCCATCGCCATG10310.10034580859158672No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)