FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3894118
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACC382700.9827642613808826No Hit
GTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGAT280350.7199319589185535No Hit
AGCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGA275670.7079138331195922No Hit
TCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCA274090.7038564316746436No Hit
GTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAA267930.6880377019905406No Hit
AGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAG260950.6701132323160213No Hit
CCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGC214250.5501887718862141No Hit
AAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTA199830.5131585637620637No Hit
GCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCT193610.49718575554207656No Hit
ACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGA161170.41388062714072865No Hit
GTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCA156320.4014259454900955No Hit
CCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCT139570.3584123542224452No Hit
GTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGA129710.33309211482548806No Hit
GAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCC123030.3159380378303893No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTG118510.3043307881271189No Hit
AGCGAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA116640.2995286737587305No Hit
ATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGT115540.29670390060085494No Hit
GGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCC111480.2862779196726961No Hit
CTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCT110440.2836072250507047No Hit
CATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCC109870.2821434789598055No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA105820.2717431777876274No Hit
AGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGC103690.2662733897637411No Hit
CTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCT96960.24899091398873893No Hit
GAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTC93430.2399259601275565No Hit
CTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGG92930.23864197232852216No Hit
CTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAG89790.2305785289505865No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT89580.23003925407499207No Hit
CCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCA86900.22315707947216804No Hit
GGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAG85880.22053774436213797No Hit
TCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCG84440.2168398595009191No Hit
ATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCG82210.21111327391722592No Hit
AGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCC81600.20954680880240403No Hit
CGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAAT80640.2070815522282581No Hit
GATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATG80580.20692747369237396No Hit
TTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTG80330.2062854797928568No Hit
GCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACAT79590.204385177850286No Hit
TCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGC79330.20371750419478812No Hit
AGCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAG78520.2016374439603525No Hit
CTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGA77840.19989122055366582No Hit
GCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGC77260.19840179470678598No Hit
GAACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGA76480.19639877374029244No Hit
ACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTA76250.1958081393527366No Hit
CACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCC75300.19336856253457135No Hit
GAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGT73550.1888746052379512No Hit
GCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACA72040.1849969620848675No Hit
CCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTG70830.1818897116112044No Hit
GTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTG69790.17921901698921297No Hit
GATTTGGTTCTTCCTTCTAAATGAGGAATCACGATCCGAGATGCAGGGCT67960.1745196216447473No Hit
GAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACG67690.17382626823326874No Hit
ATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAA67620.17364650994140393No Hit
CACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAA67160.17246524116629236No Hit
GCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCC66530.17084741653950908No Hit
CTTAAATACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAC66220.1700513441041078No Hit
AACCAAATCTTGTCCAAAAGATGTGCAGCCTCTCCCGTGTTTGTGAGGAG64920.1667129758266185No Hit
CTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACA64910.1666872960706378No Hit
GTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTT64890.16663593655867645No Hit
GTAGAAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAAT64220.16491539290797042No Hit
TCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTA63920.16414500022854983No Hit
GTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAG63370.16273261364961206No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTATTGCCTTC63040.1618851817022494No Hit
CAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCG61880.15890633000848972No Hit
GCGAAAGCAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGC61770.15862385269270218No Hit
CTCCTCACAAACACGGGAGAGGCTGCACATCTTTTGGACAAGATTTGGTT61140.1570060280659189No Hit
GAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCT60710.1559017985587494No Hit
AATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCT59660.15320542418077726No Hit
GTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGT59550.15292294686498972No Hit
CATTAGAGCACATCCAGAACTACTAGTATTGCCTTCATTTTGGTTGTTTT59520.15284590759704764No Hit
TACATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAG59020.1515619197980133No Hit
CTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTC58180.14940482029563562No Hit
AAACAAGGGTGTTTTTCCTCATATTTCTGAAATTCTAATCTTAAATACAT57180.14683684469756694No Hit
CAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACAACG55660.14293352178850258No Hit
GAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGA54710.14049394497033732No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA54130.1390045191234575No Hit
TCTCCCCCTTCTCCCCTGACAACGTCACTGAAAAGCGATGTTCTTTGGAC53370.1370528576689253No Hit
GATGTGCAGCCTCTCCCGTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCA52970.1360256674296978No Hit
AGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGG52930.13592294840577507No Hit
GCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGA52620.13512687597037376No Hit
GTGTTTGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCA52560.13497279743448967No Hit
GGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGG52550.13494711767850898No Hit
AAGCAGGGGAGTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGC52210.13407400597516564No Hit
CGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTCCTCAC51900.13327793353976433No Hit
CTGCATCTCGGATCGTGATTCCTCATTTAGAAGGAAGAACCAAATCTTGT51300.13173714818092314No Hit
GGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGG51160.1313776315971935No Hit
GCGAAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAA50990.13094107574552183No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC50870.1306329186737536No Hit
CAGGAGCAGTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCA50840.13055587940581154No Hit
GCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTG50590.12991388550629437No Hit
GACAACGTCACTGAAAAGCGATGTTCTTTGGACTTCTGCTTTCGCCTCTC50250.129040773802951No Hit
GCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCG49470.12703775283645743No Hit
CTTTGGACTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCAC49340.12670391600870853No Hit
TCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATC49150.12621600064507546No Hit
TGTGAGGAGAGGCGAAAGCAGAAGTCCAAAGAACATCGCTTTTCAGTGAC48490.12452113675035013No Hit
GTCCAAGGGATGTAACTGCCCCGAGAGGCCTCAAACTGGCCCTTTCGTGG46920.12048941506138233No Hit
ATATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAACTACTAGTAT46070.11830663580302396No Hit
CCAAAGAACATCGCTTTTCAGTGACGTTGTCAGGGGAGAAGGGGGAGAAG45980.11807551799919776No Hit
GTTCGTCTCCAAACGGAATTTCTGAATGTACTGCCCCAGCCCTGCATCTC45820.11766464190350677No Hit
GAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGACA45760.11751056336762265No Hit
GGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCTGGATG45710.11738216458771922No Hit
GGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGTGG45660.1172537658078158No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA45370.11650905288437587No Hit
TTTCTGAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATT44560.11442899264994025No Hit
AAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTAGTTCT44210.1135302011906162No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG43990.1129652465590411No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT43790.11245165143942736No Hit
GAATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAA43580.11191237656383293No Hit
GTGGCAGCAGCAGGAGCAGCAGCAGGAGCAGCAGCAGGAGCAGGAGCAGT43210.11096222559254752No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA43060.11057702925283723No Hit
AAGCAGGGCTGGGACATCGAGCTGGCCGAGGGCGACGACCTGACGTGGCG42760.10980663657341662No Hit
ACCAAAATGAAGGCAATACTAGTAGTTCTGGATGTGCTCTAATGGGTCTC42470.10906192364997672No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG42250.1084969690184016No Hit
ATGTACTGCCCCAGCCCTGCATCTCGGATCGTGATTCCTCATTTAGAAGG42200.10836857023849816No Hit
TGATTCCTCATTTAGAAGGAAGAACCAAATCTTGTCCAAAAGATGTGCAG42180.10831721072653679No Hit
AGCGAAAGCAGGGCTGGGACATCGAGCTGGCCGAGGGCGACGACCTGACG41880.10754681804711619No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT41690.10705890268348314No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT41650.10695618365956039No Hit
GGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTCTCCCCTGAC41650.10695618365956039No Hit
AGGCTGCACATCTTTTGGACAAGATTTGGTTCTTCCTTCTAAATGAGGAA41510.10659666707583078No Hit
GCAGGAGCAGGAGCAGTGGCAGCGGGCAGTATCCGAGCTTCTCCCCCTTC41280.10600603268827499No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG41090.10551811732464192No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG40740.10461932586531789No Hit
GAGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGT40640.10436252830551103No Hit
AGCAAAAGCAGGGGAAAATAAAAACAACCAAAATGAAGGCAATACTAGTA40340.10359213562609042No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA40120.10302718099451531No Hit
CTTCTGCTTTCGCCTCTCCTCACAAACACGGGAGAGGCTGCACATCTTTT40010.10274470367872776No Hit
AAAGCAGGCCAGCTTGCCGGAGTTCGTCTCCAAACGGAATTTCTGAATGT39900.10246222636294021No Hit
ATGCAGGGCTGGGGCAGTACATTCAGAAATTCCGTTTGGAGACGAACTCC39850.10233382758303679No Hit
GTGTAGAATATGTATTTAAGATTAGAATTTCAGAAATATGAGGAAAAACA39560.10158911465959686No Hit
GGGCTGGGGCAGTACATTCAGAAATTCCGTTTGGAGACGAACTCCGGCAA39300.10092144100409901No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)