FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2180504
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAG171830.7880288226942028No Hit
GTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCAAAATTTTGAATTC134660.6175636458360086No Hit
AAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCAC108090.49571108330917996No Hit
GTTAAATACTATGGCTTAAAACATTAAGCCACCAATACCAAGATTTATTG88560.40614463445148463No Hit
GTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGCGCGAGCAA84410.38711233733118583No Hit
GTATATATATAACTATCCAAACTCACATAATACTGGGACACCTGGGTGAC79380.36404427600224537No Hit
GAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCA74200.34028830031955914No Hit
CAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGGCTCCCTGCTGAGC70160.32176047372534056No Hit
ATATTATTGCTTCCACTATCAGGATTATTACCACCACCAAAAGGTGGCAT66890.3067639408136834No Hit
CCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAGAA64390.29529870158458776No Hit
CAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGCCATAGTATTTAAC62950.2886947237886287No Hit
GTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCA62850.2882361142194648No Hit
GTATTACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATT62490.28658511977047507No Hit
ATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTCAGCTC61540.28222832886341875No Hit
ATGTAATACCGATACATTTTAACTTGGCCTAAGGGATTTTTAAAATTATG61260.28094422206976No Hit
GTGAAAGATAGCCCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTC60740.27855945231010815No Hit
ACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGA60630.27805498178402793No Hit
CACTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTC60190.2760370996797071No Hit
ATAATAACTAGGATGCAGTCAGCTACACTCAATTCTCTAGTTAATATACT55230.2532900650491813No Hit
GCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAA54680.25076771241878026No Hit
TACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGATTATTACCAC53510.2454019804595635No Hit
GTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAAA52060.238752141706688No Hit
GTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAA51650.23687184247311632No Hit
GTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATG51630.23678012055928355No Hit
TTATTTATGTATGCAATAAATCTTGGTATTGGTGGCTTAATGTTTTAAGC51570.23650495481778527No Hit
GTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGTTGAT50630.2321940248676453No Hit
CATAAATAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGA49360.2263696833392647No Hit
CACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTT48840.22398491357961278No Hit
GTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCC48300.22150842190612813No Hit
GTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACT48200.2210498123369643No Hit
AAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAA45520.20875907588337375No Hit
GTATGATGATACAAGTATGCCTCATTGGATAAAAGCCACTAGCCCCATTC45390.20816288344346076No Hit
GTATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATT44760.20527364315772867No Hit
CTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCC43980.20169648851825084No Hit
TCATCATACTGCTCCCATGGAAATATGTAATACCGATACATTTTAACTTG43890.20128373990600337No Hit
ATCCATAAAAGAGCATAAAAATGAATATAACTGAAGCAAACCTGAGTTAG43590.1999079111985119No Hit
GGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAATTTGGA42760.19610145177445215No Hit
GTGTATAATGATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCA42260.19380840392863302No Hit
ACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGGCTTT41910.19220327043655963No Hit
AGTCAAAGCAGTGGATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAA41720.19133191225514834No Hit
TATTCATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACT41620.19087330268598451No Hit
AGTTATTATTATCCAACAGTGACTTTTGTTCCTTATTGGTTTAATTTGCA41600.19078158077215177No Hit
ATACAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTG39820.18261833044103565No Hit
ATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTCTAACTCAGGT39480.18105905790587865No Hit
GAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTT39260.18005011685371822No Hit
AAAATAAACTCACTTTAGTTAAATCTGCAAAAACCAGCATTAACCACTGG38620.17711501561106974No Hit
TTTCAAGGCTGATGTGCCAACAAAGTATATTAACTAGAGAATTGAGTGTA38150.17495955063599977No Hit
GCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTTTGATAGTTTGTG38120.1748219677652506No Hit
GTATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCT37940.1739964705407557No Hit
ACATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTG37900.17381302671309018No Hit
CCTTAAGACTCTTCATCTTTAAAAATTTCATTAAATTTCGGGGCGCCTGG37350.17129067408268914No Hit
GTTTCTAATCTTATGATGCAAATTAAACCAATAAGGAACAAAAGTCACTG37280.17096964738427448No Hit
GTTTTAAGCCATAGTATTTAACATGGGAGATGTTTAATTTGGTCCCGGCA37260.1708779254704417No Hit
GTGCATAGCTAATAGTTCTATTTAAGGAAAGGAGAATACACAGAGAATAT36090.16551219351122493No Hit
CATTACTACAGACCTAATAAAATCAATATTTCTATGAATGGGGCTAGTGG35910.16468669628673002No Hit
ACTATTAGCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAAT35890.16459497437289727No Hit
AGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGAATTCAAA35480.16271467513932558No Hit
GTGTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAG35330.16202676078557987No Hit
GCATTAACCACTGGCTTTCTCAAGGCACCATCAGTCACAAAGAAACAGTT34960.16032990537967368No Hit
GTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGT34900.1600547396381754No Hit
GTACTGAAGTGATAAAATTAAAATAAACTAAGAGCCTGGAAATCTAGGAA34540.15840374518918562No Hit
CCCTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAA34500.1582203013615201No Hit
GTTTAATTTGGTCCCGGCAAAATTTTGAATTCTGGGTATTTCAAGGCTGA34400.15776169179235627No Hit
ACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGCACCTGCCTTC34100.1563858630848648No Hit
CTTCAGTACATAATTTTAAAAATCCCTTAGGCCAAGTTAAAATGTATCGG34070.15624828021411563No Hit
CCATAGTACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTA34040.1561106973433665No Hit
GTATGTGGCATACCAAAAGTGCTTAAATATTATTGCTTCCACTATCAGGA33780.15491831246354054No Hit
TCCCAGGACCCTGAGCCAAAGGAGATGCTCAACCATCTGAGCCACCCAGG33700.1545514248082095No Hit
ATATCAAATAGTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCC33550.15386351045446375No Hit
CAATAATATTTAAGCACTTTTGGTATGCCACATACACTTATTTAGTCCTC33360.15299215227305246No Hit
GCCTTGAAATACCCAGAATTCAAAATTTTGCCGGGACCAAATTAAACATC32210.14771814222766846No Hit
CTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTA31840.1460212868217623No Hit
CACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTATTGGTG31630.14505820672651829No Hit
CTACTAAACTCTTTTCCTATAAGGAACTTGGAAGATTCACATTTCTTCAC31570.14478304098501998No Hit
GATTTATTGCATACATAAATAATGAAACTACCTAAGTGCATAGCTAATAG31540.14464545811427082No Hit
GCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAATGCCAC31540.14464545811427082No Hit
GTTAAAATGTATCGGTATTACATATTTCCATGGGAGCAGTATGATGATAC30620.1404262500779636No Hit
GGACATGGGAGCACAATAATGGAGGAGTGGCAGGCAGAGGTAGAAGTAGG30090.13799561936139534No Hit
GTACAAAGCACTGTGAAGAATATAAAAGAAAAAAGTAGTATTTGTAAATT29780.13657392969698748No Hit
GTTAAGCACCTGCCTTCAGCTCAGGGCTTGATCCGGAGTCCCATATCTGG29230.13405157706658644No Hit
CCATTATTGTGCTCCCATGTCCCCTGCTGCTGTGCTTGCTCGCGCTCTTG29010.133042636014426No Hit
ATTATACACAATTCCATCTCAGAATGCATCCTGTTTATTAATTTATTTGT28860.13235472166068027No Hit
ATCTGACACTTTGTATCCACTGCTTTGACTATATTCTCTGTGTATTCTCC28790.1320336949622656No Hit
GTCTTAAGGGATAAAATTAGTTTAAAGGCTTTACAAATAAATTAATAAAC28610.1312081977377707No Hit
CTTATTTAGTCCTCAAATATGTCTAAGAAATAGGTACTGTTCTTTTTCCA28330.129924090944112No Hit
GAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGATA28300.12978650807336287No Hit
ACCTACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGA28290.12974064711644648No Hit
ATTTAAGGAAAGGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACA28180.12923617659036626No Hit
ACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATACC28010.12845654032278775No Hit
ATACTGCTCCAGGCCATAGCAATCTTCTCCAAATTCTTCTGGTCATCAAT27640.12675968491688158No Hit
GCTATGCACTTAGGTAGTTTCATTATTTATGTATGCAATAAATCTTGGTA27610.12662210204613245No Hit
AGTAGGTACTATGGGCTATCTTTCACTGAGTGTTTTGCCAAATATTCTGT27550.12634693630463417No Hit
GAATATAACTGAAGCAAACCTGAGTTAGAAGAATGGAACAAAAATTTTCT27510.12616349247696865No Hit
GTACAATGCTTTTCCAGTTTTTTTCTTACTCCTTACACCCAAAAAATGAC26810.12295322549282185No Hit
TAATAATCCTGATAGTGGAAGCAATAATATTTAAGCACTTTTGGTATGCC26800.12290736453590546No Hit
GGTAGAAGTAGGCTCCCTGCTGAGCAAGGAGCCAGATATGGGACTCCGGA26580.12189842348374505No Hit
ATATTAACTAGAGAATTGAGTGTAGCTGACTGCATCCTAGTTATTATTAT26510.12157739678533036No Hit
CTTTTGGTATGCCACATACACTTATTTAGTCCTCAAATATGTCTAAGAAA26490.1214856748714976No Hit
GATAAAATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGG26460.12134809200074845No Hit
GCATTGTACTATTTGATATGATGACTTAGAAAATTTTTGTTCCATTCTTC26360.12088948243158464No Hit
ATTCTCTAGTTAATATACTTTGTTGGCACATCAGCCTTGAAATACCCAGA26330.12075189956083547No Hit
GATACAAAGTGTCAGATACTGCTCCAGGCCATAGCAATCTTCTCCAAATT26160.11997226329325697No Hit
TTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATATTA26130.11983468042250783No Hit
GTGAGTAATAGGGTGTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAG25960.11905504415492933No Hit
CATTTTAACTTGGCCTAAGGGATTTTTAAAATTATGTACTGAAGTGATAA25430.11662441343836105No Hit
CTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAAGGAGTAAG25300.11602822099844806No Hit
ATATGGGACTCCGGATCAAGCCCTGAGCTGAAGGCAGGTGCTTAACCAAC25170.11543202855853509No Hit
GGAGAATACACAGAGAATATAGTCAAAGCAGTGGATACAAAGTGTCAGAT25150.11534030664470234No Hit
AACTAAGAGCCTGGAAATCTAGGAATCCCTACTAAACTCTTTTCCTATAA25090.11506514090320404No Hit
AATTAAACCAATAAGGAACAAAAGTCACTGTTGGATAATAATAACTAGGA24920.11428550463562553No Hit
TATTTGAGGACTAAATAAGTGTATGTGGCATACCAAAAGTGCTTAAATAT24890.11414792176487637No Hit
GAAATAGGTACTGTTCTTTTTCCATTTTACAGATGAAAAGACTGAGAAAT24830.11387275602337808No Hit
GTCTGTAGTAATGTAGATTACTTTTTAGTAAAGGTAGTTTACTTGTCAGT24580.11272623210046853No Hit
ATATTTGGCAAAACACTCAGTGAAAGATAGCCCATAGTACCTACTAGTTC24520.11245106635897023No Hit
GTTTGATAGTTTGTGAAGAAATGTGAATCTTCCAAGTTCCTTATAGGAAA24390.11185487391905725No Hit
ATCTTTCACTGAGTGTTTTGCCAAATATTCTGTGAGATTTGGCACATCAT24280.11135040339297704No Hit
ATTTATTTGTCAGAGAGAGAGCAAAAGAGAGAGAGAAAGAGAGCAAGAGC24080.11043318425464937No Hit
ACATATTTCCATGGGAGCAGTATGATGATACAAGTATGCCTCATTGGATA24050.11029560138390024No Hit
CTATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAA23910.10965354798707087No Hit
GTACCTATTTCTTAGACATATTTGAGGACTAAATAAGTGTATGTGGCATA23900.10960768703015449No Hit
GCATCAGAATCATCTGGGATCACTTGGAGAAGTAAACTACAGATTCTGCA23870.10947010415940535No Hit
ACTATATTCTCTGTGTATTCTCCTTTCCTTAAATAGAACTATTAGCTATG23850.10937838224557257No Hit
GTTATATATATACAATGAACTAATTATAAAGGAATATACAATGAAAGTTA23610.1082777192795794No Hit
ATTACTCACTGTCTCTACTGGGTACAAAGCACTGTGAAGAATATAAAAGA23340.10703947344283707No Hit
CTATCAGGATTATTACCACCACCAAAAGGTGGCATTGATGACCAGAAGAA23060.10575536664917835No Hit
TAATGAAACTACCTAAGTGCATAGCTAATAGTTCTATTTAAGGAAAGGAG23020.10557192282151283No Hit
AATCTGTACTTTAAAACAAGCAAAATTAAGTTGTCATTTTTTGGGTGTAA22820.10465470368318516No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG22750.10433367698477049No Hit
CAGATGATGTGCCAAATCTCACAGAATATTTGGCAAAACACTCAGTGAAA22750.10433367698477049No Hit
TTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAAAGAACA22730.10424195507093774No Hit
TACTAGTTCCTACTCTTATTTCTCAGTCTTTTCATCTGTAAAATGGAAAA22490.10314129210494455No Hit
ATTAGAAACTGTTTCTTTGTGACTGATGGTGCCTTGAGAAAGCCAGTGGT22480.10309543114802816No Hit
ATTCTTCACAGTGCTTTGTACCCAGTAGAGACAGTGAGTAATAGGGTGTT22130.10149029765595477No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT22090.10130685382828925No Hit
ATCCAAACTCACATAATACTGGGACACCTGGGTGACTCAGTTGGTTAAGC21960.10071066138837627No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)