FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences885203
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCTAGGGAGGAGCCAGCCTGTGGGGCAGGGAGAGGAGGGCTGCAGCACC53620.605736763205728No Hit
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG36710.41470713497355977No Hit
CTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGGGCCGGGTGAT30470.34421482981869694No Hit
TCCCTAGGCTGTGTAATTTAAGCCACAGCCCCACCTTCCCCATATCATGT27550.3112280459962291No Hit
CCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGGGGAGAGACGGCCTGCAC27520.31088914068298457No Hit
GTGCAGTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCC23130.2612959965115346No Hit
GCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACCAGTCC19990.22582390705860692No Hit
GTACCAGGGATGTGAGCAGAAATGACAACGGCTTTCTAAGTGCAAGGCAA19920.22503312799436967No Hit
GGCCTAGGGAGCGTGTGTGTCTAGAGTGTGAATGGATCACAGATGAATGA19680.2223218854884134No Hit
CTCTAGACACACACGCTCCCTAGGCCCAGGGGTTCCTGGTCTGTAAATGG16880.1906907229189237No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG16320.18436449040502573No Hit
ATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGT16090.18176621633681764No Hit
CCCTGGTACCTAGCAAAATGCCATGCGCACTAAATGGCCAGGATGACTCA14710.16617657192756916No Hit
CTGTGAGACCTGCCTGGCCCTGCTTTTGGTCTTATTAACCCACTCAGTTC14160.15996330785141938No Hit
GTCTCACAGTGAGCAGACTGGCCTGGTGGAACTTGGAACCAACTAGAGAT14080.15905956034943397No Hit
GTGCTGGGCAGGGGGTGAACACTCCCTGTTCCTGGGTCTTCTCCCTCCAA13540.15295926471103238No Hit
GGTCAGGAGTGGGGACTTTATTCAACATGTGATGGGAAGCCTGGGGAAGG13270.1499091168918316No Hit
GTGTGTATGTGTGCATGTGGGTGTGTAAACTGCTTTCCCAATACCAGTAG11960.13511025154682033No Hit
GTCTAAGGGTTGACTTTTCTACAATAAGCTGCCCGATTTAATCAAGAGGA11670.13183416685212318No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGATAGATGCTTAATGAC11370.12844511371967784No Hit
GCCCAGCACTGCACTGAGCGTTTTGTGGATTGTCTCATTCAGTGTTCACC11110.12550793433822524No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC11100.12539496590047708No Hit
TGTCTAGAGTGTGAATGGATCACAGATGAATGAACACAGAGCCCATGGGT11070.12505606058723254No Hit
GGGTAAAGGCTTGGGGCCCAAGGGTAGAGGAAAGATGGCTCTGAAACCAC10930.12347450245875805No Hit
CCCATGGGCTCTGTGTTCATTCATCTGTGATCCATTCACACTCTAGACAC10860.1226837233945208No Hit
GCCCATGGGTCCTCATTTCCACTTGGTCCCAACTGGCTGAGGGGTGGCTG10540.11906873338657913No Hit
GTCCTTTAGTTCTCCCAACAATGCTGCAGTGCAGGCCGTCTCTCCCCATT10260.11590561712963014No Hit
AAACTATACAGGTCAGTGTGAATGACATCCGAATTATATTAGTGGAAAAA10060.1136462483746666No Hit
CTCCTGACCATGGCCAGCGAGATCAAGAGGGACCTGCTCCCTCCTTCCCC9910.11195172180844394No Hit
CCTCTAAGGCAGCTTCCTACTATGGGGGTTGTTTCCCATTTAGGGACCAG9840.1111609427442067No Hit
AGCAGGGACAGTGGCAGAGGGAGAAGCAGGCTCCCCACTGAGCAGGGAGC9710.1096923530534804No Hit
CTTTAAGCCCAGGCGTGCCGTGGTCTGATCTATGGATTTAAAAATCCTCC9630.10878860555149497No Hit
GCTCTGGACACTACTGGGCTGTGGCTCCTTCTCCCCCTGGAGAATGGAGG9610.1085626686759986No Hit
ATATGGGGAAGGTGGGGCTGTGGCTTAAATTACACAGCCTAGGGAGGAGC9450.10675517367202778No Hit
GCCCTGAGGACGTCAGGAGTGAAGCACATGATATGGGGAAGGTGGGGCTG9340.10551252085679783No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA9190.10381799429057514No Hit
TATCAAAACAGCCACTCTTGTTGGGAAACAAATCGTGGAATGGATCTTGA9140.10325315210183428No Hit
TCCCCATATCATGTGCTTCACTCCTGACGTCCTCAGGGCTGAGGGCTGTT9110.10291424678858974No Hit
GTGTGAATGACATCCGAATTATATTAGTGGAAAAACTTGTTCCTACACTC9000.10167159397335979No Hit
CTAAAGGACAGATGGGGTGCAAAGCTTACCCTTTAAAGGGTGCTGCAGCC8900.100541909595878No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)