FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1383538
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGC96980.7009565331779828No Hit
CCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAATCTG93000.6721897049448587No Hit
GTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAG72150.5214891098039953No Hit
GTGCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTG70440.5091294926485576No Hit
CTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAGGGGAGCAG63280.45737811321409316No Hit
CTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCC61270.44285014217173657No Hit
CTCCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCC60940.44046495289612575No Hit
GTTCTGTACTGTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACA57840.41805862939796373No Hit
AATTAACACTGGCCATTAAGAAGCTGCGGTGAGTAAAACGTCAAGCTCAG53520.3868343334263316No Hit
CCTGAGAACTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCC50760.36688547766667773No Hit
GGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCA47720.3449128249458996No Hit
GTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGC47040.3399978894688834No Hit
GTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGG46540.33638396632401857No Hit
CCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACAC46090.3331314354936402No Hit
GTCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGT42130.30450916418631074No Hit
GTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATGGG41610.30075068411565137No Hit
GGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAA41580.30053384872695943No Hit
ATCTTGCAGCTCCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTG41080.2969199255820946No Hit
GAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACC40540.29301688858564057No Hit
GTGTAGGGACATGGCATCAAAGTTCTGGCAAGGAGGGGCGCCTGGGTGGC37360.27003233738430027No Hit
CCCCTGGGGCTGGGGCTGAGTACAGTTCTGCTGGCCCTGCTCTACAGCAC36660.2649728449814895No Hit
ACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCT35130.2539142401582031No Hit
TTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAG33960.2454576599992194No Hit
GTGTTAATTTGTGTTATTTCTTAAGCAATTTACTCGATAACGTTTTGTTA33440.24169917992856No Hit
GTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCAC32860.23750702908051677No Hit
GCTCTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTA32630.23584462443387894No Hit
CCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGT31850.23020690432788982No Hit
GCCCCAGCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGG31330.22644842425723036No Hit
TCCCTACACATAGTGATCTCTTCTGTGCAGTATTGTTAGTGAAGCTCGTT31290.2261593104056412No Hit
TTCCAGGAGAGAGCAGGAGGCCTGACAGTTTGCGGGAGCCCTGGAGGAAA30720.22203943802049528No Hit
CTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGA30610.22124437492862503No Hit
CCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGAGCCTCTCC30610.22124437492862503No Hit
CACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACT29350.21213728860356565No Hit
CTATAAAACACGTCAAATGTCAAGTTTATAACAAAACGTTATCGAGTAAA29320.21192045321487374No Hit
GGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGC29250.21141450397459266No Hit
CTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCC29200.21105311166010618No Hit
CTCCAGGATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGA29110.2104026054940305No Hit
GTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAG28710.20751146697813866No Hit
CATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAG27990.20230741764953328No Hit
CCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAGAGTCAAGAAT27910.20172918994635491No Hit
ATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGC27640.19977767144812789No Hit
ACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGA26650.19262210362129556No Hit
ACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCC26620.19240526823260365No Hit
GTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTCACTATCAG26610.19233298976970636No Hit
GCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGT26430.191031977437555No Hit
CCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTG26100.18864678816194425No Hit
GATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAA25750.18611704196053885No Hit
GGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGG25590.1849605865541821No Hit
GAATTAAAGAGTCAAGAATCTGAAGGCTTTTCAGACAGAACAGGTCCCTA25390.18351501729623618No Hit
CCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCCCG25330.18308134651885238No Hit
GTTTATAACAAAACGTTATCGAGTAAATTGCTTAAGAAATAACACAAATT25100.18141894187221455No Hit
GCTCTACAGCACGTGGGGACTCTGCCTGTGTGCCCCCACCAGCATTATCA24700.1785278033563227No Hit
GGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACC24300.17563666484043083No Hit
GTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTG24120.17433565250827948No Hit
GCCACACAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAA23820.1721672986213606No Hit
GCTCTATGGAGGTCACTCACTGTGTGGCACCGGGCCTCATGAAGTTGTAG23760.17173362784397683No Hit
GAGCCCAACCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTG23520.1699989447344417No Hit
GGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCC23410.16920388164257144No Hit
CCCCAGATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGAT23010.16631274312667957No Hit
GTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTAGTCTTGTGTC22860.1652285661832201No Hit
TCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATT22350.161542364575458No Hit
TCCCAGGACCCTGGGATCATGACCCAAGCTGAAGGCAGAGGCTTTAAGCC22320.1613255291867661No Hit
GCCCCAGGGGAGCTGCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTG22260.16089185840938233No Hit
CTTCTGTGCAGTATTGTTAGTGAAGCTCGTTGGCCTGGCAGGATCAGGTA21860.15800071989349046No Hit
CTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCAGGAGAGGCT21860.15800071989349046No Hit
GTATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACA21710.15691654295003102No Hit
CAGGCAGAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCA21700.1568442644871337No Hit
GTTCTGAATTTGTAACATCTGGTCTCTGCTCAGGCGAACAATTTCACAAA21640.15641059370974994No Hit
TCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACA21620.15626603678395534No Hit
ATCCCATCCCCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAAT21610.15619375832105803No Hit
GTGTGGCACCGGGCCTCATGAAGTTGTAGATCCAGCTCCCACCCAGAGAA21200.15323034134226887No Hit
AAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGG21190.15315806287937156No Hit
GGATTACAATTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGT21180.1530857844164743No Hit
GGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAAT20890.15098970899245268No Hit
GGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATC20860.1507728736037608No Hit
GCCTCAGACTCCCCACGCTGAGCCCAGGCCCCTACCCTCTGAAGGTGTTT20820.1504837597521716No Hit
ATCTACAACTTCATGAGGCCCGGTGCCACACAGTGAGTGACCTCCATAGA20820.1504837597521716No Hit
CCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACACACCCCA20810.1504114812892743No Hit
GCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCTTGTGGGGTGT20680.14947186127160947No Hit
CTCCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCA20650.14925502588291756No Hit
AGCAGGAAGTGCCACACTTTCAAACCATCAGCTCTCCTGAGAACTCCCTC20620.14903819049422568No Hit
ATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCCCTCCCA20500.14817084893945812No Hit
CTTGTGGGGTGTGTGTGTCAGAATCACATGGGAAACACCTTCAGAGGGTA20400.14744806431048516No Hit
GTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCA19780.14296679961085276No Hit
GTACTCAGAAGGGGAAGGCAGGAGAGGCTCCTTCTACTGCCAGCCGGAGC19520.14108755957552302No Hit
CTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGC19420.14036477494655009No Hit
CCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGAT19350.139858825706269No Hit
TTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGG19310.1395697118546798No Hit
CTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCTGGAATTGTAA19300.13949743339178253No Hit
GCTCATTAGACATCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAG19280.13935287646598793No Hit
GGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTG18790.13581123178402038No Hit
GCCCAGGCCCCTACCCTCTGAAGGTGTTTCCCATGTGATTCTGACACACA18740.1354498394695339No Hit
GAACAGGTCCCTAGAGCCCTGCGTGTCTGCTCCGGCTGGCAGTAGAAGGA18500.13371515635999878No Hit
AGCGAAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCA18010.13017351167803123No Hit
GGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCAC17990.13002895475223664No Hit
GATAAGGAGGGGGCGGCTCATTAGACATCTTTGCTGCTTCTGGACCTCAA17910.1294507270490583No Hit
CCTCCCACCAGGTTCCTCCCTTGACACAGGAGGATTACAATTCCAGATGA17810.1287279424200853No Hit
TCTTTGCTGCTTCTGGACCTCAAATCCCAGGTACTCAGAAGGGGAAGGCA17800.12865566395718803No Hit
AGCAGGTGCAGTGCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACT17740.12822199317980423No Hit
GTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGTGT17660.12764376547662587No Hit
AAAGCAGGGTGGGAGGGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGC17580.12706553777344748No Hit
CCCTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATC17500.12648731007026914No Hit
GACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCC17460.12619819621867992No Hit
GACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAATCTCATCT17360.125475411589707No Hit
AGCGAAAGCAGGGCCTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTT17070.12337933616568537No Hit
GTCAGGGCCAAAATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGG16710.12077731150138268No Hit
CTAGGGACCTGTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATT16700.12070503303848538No Hit
TTCCAGATGAGATTTCGGTGGGGACACAGAGCCCAACCTGTGAGGGTCTC16660.12041591918689622No Hit
GCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACTCCCCACGCTGAG16580.11983769148371784No Hit
GCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCT16060.1160792114130584No Hit
GAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGGAGCTGGATCTACAAC16020.11579009756146923No Hit
GAGTACAGTTCTGCTGGCCCTGCTCTACAGCACGTGGGGACTCTGCCTGT16010.11571781909857193No Hit
CAGTGAGTGACCTCCATAGAGCGACCCACGAAATGTTGGCTGAATTAAAG15920.11506731293249625No Hit
CAGCAGAACTGTACTCAGCCCCAGCCCCAGGGGAGCTGCAAGATAGACTG15820.11434452830352329No Hit
CTCACTATCAGGGGAGCAGCAGGGGGGAAACTGTCCCCAGATCCCATCCC15760.11391085752613951No Hit
GTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCGGTGATAAT15300.11058604823286386No Hit
AAAGCAGGGAGCCTTAGCTAAATGCTGTATGTCATAGATCATCTGGTTCT15270.11036921284417198No Hit
CTGTGTCCCCACCGAAATCTCATCTGGAATTGTAATCCTCCTGTGTCAAG15230.11008009899258278No Hit
ATCAGGAGGCGGTGATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCAC15200.1098632636038909No Hit
GGCCTGCAGGGCCATGAGTGGGAGCCCTCCTCCAGTGTTCTCTGGGTGGG15080.10899592204912333No Hit
CCTGTGAGGGTCTCAGTCTATCTTGCAGCTCCCCTGGGGCTGGGGCTGAG14750.10661073277351255No Hit
CTTTAATTCAGCCAACATTTCGTGGGTCGCTCTATGGAGGTCACTCACTG14700.10624934045902606No Hit
GATAATGCTGGTGGGGGCACACAGGCAGAGTCCCCACGTGCTGTAGAGCA14590.10545427736715579No Hit
GAGTCCCCACGTGCTGTAGAGCAGGGCCAGCAGAACTGTACTCAGCCCCA14430.10429782196079906No Hit
CCTCCAGAAGGGGGGCTGTGGGGCCTCCAGGATAAGGAGGGGGCGGCTCA14390.10400870810920987No Hit
GTGCCCAGCTCCTCCCACTCTGCACCTGCTGCCTCAGACTCCCCACGCTG14320.10350275886892879No Hit
GGGGCAAGGTTACCAGGAAGCAGGGCTGTCAGGGCCAAAATCAGGAGGCG14280.10321364501733961No Hit
GTTCTGTCTGAAAAGCCTTCAGATTCTTGACTCTTTAATTCAGCCAACAT14200.10263541731416123No Hit
ATGTCATAGATCATCTGGTTCTTGTGGGGTGTGTGTGTCAGAATCACATG14120.10205718961098285No Hit
CCTTGACACAGGAGGATTACAATTCCAGATGAGATTTCGGTGGGGACACA14080.10176807575939367No Hit
GTTCTCTGGGTGGGAGCTGGATCTACAACTTCATGAGGCCCGGTGCCACA14070.10169579729649637No Hit
GCAAGATAGACTGAGACCCTCACAGGTTGGGCTCTGTGTCCCCACCGAAA13950.10082845574172883No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)