FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3910951
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAG579701.4822481795348497No Hit
CTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCT522771.3366825613514461No Hit
GAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCC484961.2400053081718487No Hit
GACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGA376000.961402993798695No Hit
CTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATG290540.7428883665379596No Hit
CTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCT272290.6962245244187412No Hit
ATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTC249480.6379011140768576No Hit
TCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCT225610.5768673655077754No Hit
CCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTC207760.5312262925308959No Hit
GGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGA204260.5222770625354294No Hit
AAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAG202820.5185950936230088No Hit
AAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCT195320.4994181722041519No Hit
CTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCT191530.4897274345804895No Hit
CTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCC185770.47499955893080736No Hit
GAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAA172610.44135045414785307No Hit
AGCGAAAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGG164230.41992344061585024No Hit
TAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGA163720.41861940995936797No Hit
ACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCA159030.40662744176544274No Hit
GAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAAT158930.40637174947985794No Hit
AACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATC154620.39535141197115486No Hit
GACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGC143230.3662280606430508No Hit
GAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCC138140.3532133233067865No Hit
CTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACT134120.3429344934262792No Hit
CTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTT133800.34211627811240797No Hit
CCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTA124720.3188994185813118No Hit
CCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGA119400.3052965889882026No Hit
GTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGG112960.2888300057965441No Hit
CCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTAC110140.2816194833430539No Hit
CCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCC107740.27548286848901965No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA103510.26466708480878437No Hit
CTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGGTATAT99770.25510419332791434No Hit
GGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAG98770.25254727047206676No Hit
GTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAG96490.24671748636073426No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA96160.24587370181830456No Hit
ATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAG90490.23137594922564872No Hit
TCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTT88840.22715702651350017No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT85650.21900044260334633No Hit
CTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCAC84930.21715945814713608No Hit
CCCCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTG83040.21232687394958416No Hit
CCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCA79130.20232930558322001No Hit
GGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATTCA76220.19488866007270356No Hit
TAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGG74360.19013278356082702No Hit
CTGCACGCTCCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTG74310.19000493741803465No Hit
AGCGAAAGCAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCC74130.18954469130398208No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT74050.18934013747551426No Hit
TACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAA73240.1872690299622777No Hit
GGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTA72010.18412401484958518No Hit
GGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACC68950.17629983091069154No Hit
GGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCAGAGAGAG67770.1732826619407914No Hit
GAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAG67660.17300140042664813No Hit
CTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTCCC65380.16717161631531563No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT64010.16366863200280443No Hit
AATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTT61310.15676494029201593No Hit
CATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTG60150.15379890977923272No Hit
ATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCC58590.1498101101241105No Hit
AAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCCAGATT58480.14952884860996724No Hit
ACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTCCCTTGGTTCCGG57680.14748331032528916No Hit
GGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCT56210.1437246337271932No Hit
CCACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCT56160.1435967875844008No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT55770.14259958767062025No Hit
GGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGAGGCAAAGGAGAGGA55560.14206263387089227No Hit
CCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCC55420.14170466467107362No Hit
GTAAGTAATCATAGAGGGAGGCAAAGGAGAGGAGAGGAAGCATTCCCCAC54750.13999152635765572No Hit
TAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTC54630.13968469561495403No Hit
CCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCC53870.13774143424450982No Hit
GGGAGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAG53800.13756244964460052No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT50940.13024965027687638No Hit
GGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTTGCCTC50320.12866435810625088No Hit
AGGCAAAGGAGAGGAGAGGAAGCATTCCCCACAAGGGTCTGTAGGAGCCC50300.12861321964913394No Hit
CCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTCCACTCAGTCTC50220.12840866582066612No Hit
CTTCCTCTCCTCTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCT48550.12413860465140066No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC48380.12370392776590655No Hit
CAGGCAGACTGGGACCGTCACCTCCCTCTCTCTGCACGCTCCACCTCTAT46760.11956171273943346No Hit
AGACTGAGTGGAGGGGTGAACAGACCCAGAAGTAAGTAATCATAGAGGGA45900.11736275908340453No Hit
ACCTCTATTAATGAATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTC44210.11304155945702209No Hit
AATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGAACAGACCCAGAA44190.11299042099990513No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC43960.11240232874306019No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC43140.11030565200126517No Hit
GTCACCTCCCTCTCTCTGCACGCTCCACCTCTATTAATGAATCTGGGCTC42780.10938515977316005No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA42420.10846466754505489No Hit
AAGCAGGGGAATAATATACCGGAACCAAGGGAGACTGAGTGGAGGGGTGA41870.10705835997433873No Hit
CTCCTTTGCCTCCCTCTATGATTACTTACTTCTGGGTCTGTTCACCCCTC41580.10631685234614291No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG40770.10424574483290637No Hit
CATTAATAGAGGTGGAGCGTGCAGAGAGAGGGAGGTGACGGTCCCAGTCT40650.10393891409020464No Hit
CCACAAGGGTCTGTAGGAGCCCAGATTCATTAATAGAGGTGGAGCGTGCA40410.10332525260480123No Hit
ATCTGGGCTCCTACAGACCCTTGTGGGGAATGCTTCCTCTCCTCTCCTTT40240.10289057571930714No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)