FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1004045
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA188881.8811905840873666No Hit
TCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGG102111.016986290455109No Hit
GGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTG71780.7149081963457813No Hit
GTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGA66710.6644124516331439No Hit
ACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGC66260.6599305808006614No Hit
GCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGATC59000.5876230647032753No Hit
CTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTTG50920.5071485839778097No Hit
GTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCT48620.48424124416734315No Hit
GTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCA48420.48224930157512863No Hit
GGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATC46050.4586447818573869No Hit
AGCGAAAGCAGGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCC45650.4546608966729579No Hit
GTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCT43590.4341438879731486No Hit
ATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGG42930.4275704774188408No Hit
CCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTA39270.3911179279813156No Hit
TTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGAT39130.38972356816676545No Hit
GCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGT38670.3851421002046721No Hit
GTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCA37310.3715968905776136No Hit
CCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCC32170.3204039659577011No Hit
GGGCAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACT31840.3171172606805472No Hit
CCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTC31560.31432854105144686No Hit
GGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTT31090.30964747595974285No Hit
AGCGAAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGA29960.29839300031373095No Hit
GTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCA29540.2942099208700805No Hit
CTGTTACACCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAA28510.2839514165201759No Hit
GCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGC28190.2807643083726327No Hit
TCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTG28000.27887196291002897No Hit
GCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATA27750.27638203466976086No Hit
AAAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTT27710.27598364615131793No Hit
GCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATGCTAGGGC27440.2732945236518283No Hit
GCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACA27300.2719001638372782No Hit
CATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGAC27110.2700078183746744No Hit
GCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTT26780.2667211130975205No Hit
ACTGTAAGCTCCATAAAGGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGG26280.26174125661698433No Hit
TATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGG25830.2572593857845017No Hit
TATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTT25670.2556658317107301No Hit
GTGCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCC25410.25307630634085126No Hit
CTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAG25170.25068597523019387No Hit
GCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGG24660.24560652162004692No Hit
TGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGG24560.2446105503239397No Hit
ACCCAGCAGGGAGCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCAT24230.24132384504678572No Hit
GGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTG24170.2407262622691214No Hit
GAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGA23990.23893351393612836No Hit
CCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAG23970.2387343196769069No Hit
AGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGC23630.2353480172701423No Hit
GGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCC23360.2326588947706527No Hit
CCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGG22810.22718105264206284No Hit
ATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCG22360.22269918180958026No Hit
TCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGC22350.22259958467996951No Hit
CTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAAC22100.2201096564397014No Hit
CTTTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCA22100.2201096564397014No Hit
ACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCT22090.22001005931009068No Hit
GCCCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTA22060.21971126792125853No Hit
AGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCAT22010.2192132822732049No Hit
GATATCAGCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCAT21430.21343664875578286No Hit
CATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCTCAGTCGTTGGGCGTCTG21400.2131378573669507No Hit
GAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCC21360.21273946884850778No Hit
GCCCTGAGCATCCATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTT21330.21244067745967563No Hit
GGGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCA21300.21214188607084344No Hit
GCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCC20940.20855638940485732No Hit
GTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGAT20510.20427371283159618No Hit
GTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAA20080.19999103625833503No Hit
ACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCGCCTCTC19500.194214402740913No Hit
TCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCCAACGAC19450.1937164170928594No Hit
AAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGC19390.19311883431519503No Hit
GGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTT19380.1930192371855843No Hit
GAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCC19030.18953333764920896No Hit
GTCACTGCCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACT18990.18913494913076603No Hit
TATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCA18730.1865454237608872No Hit
GATCTGCCCGGTCAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGC18690.18614703524244433No Hit
GCCTTAGACCTAGGTCATGATCCTGGGGTCCTGGGATTGAGCCCTGCATG18580.18505146681672635No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA18520.184453884039062No Hit
CCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCT18440.1836571070021762No Hit
CAATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTC18020.17947402755852576No Hit
GCCCAGGGCCTTGGCACTTTAAAAAGCCTCAATAAGCATCGAAAGTATTC17710.17638651654059329No Hit
AGCGAAAGCAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGC17690.17618732228137185No Hit
GTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACA17490.17419537968915735No Hit
GCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATA17420.17349819978188227No Hit
TTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGA17310.17240263135616432No Hit
GAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGC17250.17180504857849999No Hit
TCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGT17160.17090867441200344No Hit
AATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGC17130.17060988302317126No Hit
TTAAAAAGCCTCAATAAGCATCGAAAGTATTCCCAGGCAACTCTCTCAAC16860.1679207605236817No Hit
AGCGAAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCA16760.16692478922757445No Hit
ATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCA16730.1666259978387423No Hit
GAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGAT16680.16612801219068868No Hit
ATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGCG16610.1654308322834136No Hit
GGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGA16470.16403647246886344No Hit
GGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTGGGAGGCT16270.16204452987664894No Hit
CTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGT16150.16084936432132027No Hit
ACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCTCCATAAAGGTAGGA16090.1602517815436559No Hit
CTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGGC16090.1602517815436559No Hit
CTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACA16070.16005258728443444No Hit
GGCTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCAC16070.16005258728443444No Hit
CCCATGCAGGGCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAG16030.15965419876599157No Hit
TTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAATGGTGTAACAGA16030.15965419876599157No Hit
GCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGATG15810.1574630619145556No Hit
GGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGC15790.15726386765533418No Hit
GCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAG15730.15666628487766984No Hit
GATCATGACCTAGGTCTAAGGCAGACGCCCAACGACTGAGCCTCCCAGGC15710.15646709061844838No Hit
CGTTAACGTAATTAATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGG15640.1557699107111733No Hit
GGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCC15630.15567031358156258No Hit
GCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAAAA15430.15367837098934808No Hit
CTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCA15310.1524832054340194No Hit
TCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAACGTTT15190.1512880398786907No Hit
GGTAGGAAGCGCCTTCTGTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCT14970.14909690302725476No Hit
GCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCT14950.14889770876803332No Hit
GTGCCAAGGCCCTGGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTT14870.1481009317311475No Hit
GCTCAATCCCAGGACCCCAGGATCATGACCTAGGTCTAAGGCAGACGCCC14620.1456110034908794No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT14580.1452126149724365No Hit
AAAGCAGGGGGAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGA14570.14511301784282576No Hit
GCATATGGATGGATGCTCAGGGCTGATATCTGAGCCGAGGCTATAGCCCT14380.143220672380222No Hit
GAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCAGGGCTCAAT14210.14152752117683967No Hit
GACAGAAGGCGCTTCCTACCTTTATGGAGCTTACAGTCGAGCGAGGGAGG13960.13903759293657156No Hit
GGCTATAGCCCTCCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTC13540.13485451349292113No Hit
ATGCTAGGGCAGTGACTGCAGCCACATTAAAGAGGGAAGAGAGGCGCCTG13520.1346553192336997No Hit
TTATTGAGGCTTTTTAAAGTGCCAAGGCCCTGGGCAGACAGCCAGGAAGG13110.13057183691965998No Hit
GGGCTGATATCTGAGCCGAGGCTATAGCCCTCCTCTCGACTGGCTCCCTC13040.1298746570123849No Hit
CCCAACGACTGAGCCTCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTG12770.12718553451289533No Hit
TCCCAGGCGCCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAG12750.1269863402536739No Hit
CTGCTGATGACTGTTTTCTGGACGCCCTCGCAGATCTGCCCGGTCAGCAA12740.12688674312406317No Hit
TCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACGTTAAC12640.1258907718279559No Hit
GCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGATGCTTATTGAGG12580.12529318905029158No Hit
CAGGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGT12550.1249943976614594No Hit
CAGCAAAATGGTGTAACAGAAATGCCCCAGTGCTCAGCTGTGCATATGGA12390.1234008435876878No Hit
CCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGATATCTGA12360.12310205219885563No Hit
TCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGC11860.11812219571831939No Hit
CCATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATC11690.11642904451493709No Hit
GGGCAGACAGCCAGGAAGGGAAAGACAGAAGGCGCTTCCTACCTTTATGG11680.11632944738532636No Hit
GGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCTCTGCTGATGA11630.11583146173727273No Hit
TTATGGAGCTTACAGTCGAGCGAGGGAGGCAAACGTTAACGTAATTAATG11460.11413831053389042No Hit
CTCTGCCACATCCTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGC11380.11334153349700461No Hit
GAGTGGGAGGAGGAGAAGCAGGCTCCCCACCCAGCAGGGAGCCCCATGCA11380.11334153349700461No Hit
GAAATGCCCCAGTGCTCAGCTGTGCATATGGATGGATGCTCAGGGCTGAT11300.11254475646011881No Hit
CCTCTCGACTGGCTCCCTCACCCTCACAGAGGAAACTCAAGCCACGGAGG11280.11234556220089736No Hit
CCCTAGCATTAATTACGTTAACGTTTGCCTCCCTCGCTCGACTGTAAGCT11250.1120467708120652No Hit
CCTCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTA10960.10915845405335418No Hit
TCTCTTCCCTCTTTAATGTGGCTGCAGTCACTGCCCTAGCATTAATTACG10900.10856087127568982No Hit
GCCTGGGAATACTTTCGATGCTTATTGAGGCTTTTTAAAGTGCCAAGGCC10890.10846127414607912No Hit
ATCCATATGCACAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCT10790.10746530284997187No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT10710.10666852581308606No Hit
CTCCGTGGCTTGAGTTTCCTCTGTGAGGGTGAGGGAGCCAGTCGAGAGGA10710.10666852581308606No Hit
ATTTTGCTGACCGGGCAGATCTGCGAGGGCGTCCAGAAAACAGTCATCAG10630.10587174877620027No Hit
GTCTTTCCCTTCCTGGCTGTCTGCCCAGGGCCTTGGCACTTTAAAAAGCC10610.10567255451697881No Hit
CAGCTGAGCACTGGGGCATTTCTGTTACACCATTTTGCTGACCGGGCAGA10370.10328222340632143No Hit
GGGTGAGGGAGCCAGTCGAGAGGAGGGCTATAGCCTCGGCTCAGATATCA10300.10258504349904636No Hit
AAGCAGGCCATCCTTGTTCCCTCTGCCACATCCTCCGTGGCTTGAGTTTC10180.10138987794371765No Hit
GGAGGGTAGAGGAAGGGGCATGGAAGGAGCCACTCTGGGCTGGGCTGTCT10150.10109108655488548No Hit
CTCTGGGCTGGGCTGTCTCTGCTGATGACTGTTTTCTGGACGCCCTCGCA10100.10059310090683186No Hit
CTATAGCCTCGGCTCAGATATCAGCCCTGAGCATCCATCCATATGCACAG10100.10059310090683186No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)