FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H10.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_H10.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6600702
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA864761.3101030769151523No Hit
GGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCA742861.1254257501702092No Hit
CAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTT674971.0225730535933906No Hit
ATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTT590270.8942533688083479No Hit
TAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAG484070.7333613909550832No Hit
ATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCT481620.7296496645356811No Hit
AGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAA423610.6417650728664921No Hit
CCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACA399640.6054507535713626No Hit
TTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTT385070.5833773438037348No Hit
GCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGAT344060.5212475885140702No Hit
GTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTC335090.5076581248479328No Hit
TATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAAC331560.5023102088232433No Hit
CTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTC330840.5012194157530517No Hit
ATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAA324080.49097808081625255No Hit
ATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGAC317610.4811760930882806No Hit
ATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCA314000.4757069778335698No Hit
CTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTG312900.47404048842077706No Hit
TATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCA306860.46488994655416954No Hit
GTTTTATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGC299380.45355781854717875No Hit
AGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAGGCACCATAAAA281690.4267576388087207No Hit
GATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTA271680.4115925851523065No Hit
GCTTTATCTAGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGT267490.40524477547994137No Hit
GTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAG257540.3901706212460432No Hit
ATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAAT250860.38005048553926535No Hit
CTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAG249630.37818704737768805No Hit
CAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAA245170.371430190304001No Hit
GTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTC244880.37099084309517383No Hit
AGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATC229020.34696309574345274No Hit
CTCTTTCTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGT227070.3440088645116838No Hit
GGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTT226330.3428877716339868No Hit
GACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTT221160.3350552713938608No Hit
CTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAA203720.3086338392492193No Hit
GTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTT196120.29711991239719654No Hit
GCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTG187890.28465154160875616No Hit
ATTATATTCCTGGCTTTAAAGTTGAGAGAGTTGCCTGGGAATACTTTCGA185150.2805004679805269No Hit
AGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCA181320.2746980548432576No Hit
AGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTT178430.27031973265873843No Hit
TCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTG178310.2701379338137065No Hit
GCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAA174120.26379012414134134No Hit
CTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCT172720.2616691376159687No Hit
GTACTAAGGCACCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAG169450.2567151190888484No Hit
TCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCT165680.25100360537409505No Hit
AGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGA163990.24844327163989527No Hit
GGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAG162530.2462313856920067No Hit
CTCTCTCTCTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTC161370.24447399685669796No Hit
GATTAATATCTAGATAAAGCATTCACAAAAACCAATTCAAAAGTACTAAG159340.24139856639490773No Hit
GATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAA159210.2412016176461231No Hit
CTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCACTA155830.23608095017772354No Hit
TCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTAC153900.23315701875345987No Hit
ATTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTAT150960.22870294705017738No Hit
GCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGAC149140.225945664567193No Hit
GCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGATGTAGCA148470.2249306210157647No Hit
GTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATT146970.22265813545286547No Hit
CCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATCTAGATATTAAT145510.22044624950497688No Hit
ATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAA144490.21890095932220544No Hit
ATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCA141340.21412873964011706No Hit
GATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAACATGCACAACTGAA140080.21221985176728175No Hit
CACCCAGGCATCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTT137140.20776578006399923No Hit
TAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTCAGCTC136940.207462781988946No Hit
TTTTTATGGTGCCTTAGTACTTTTGAATTGGTTTTTGTGAATGCTTTATC135650.20550844440485272No Hit
GCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGC134930.20441765133466108No Hit
AATCAAAAGGACTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGG134710.20408435345210252No Hit
GGTCATGATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCC133850.2027814617293736No Hit
GTGGAGAGCCTCCTTCTCCCTCTTCTGTTTCCCCTGCTTGTGCGCTCTTT133590.20238756423180443No Hit
GAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTC131940.1998878301126153No Hit
CTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTA130580.19782744320225334No Hit
CTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGT130450.19763049445346872No Hit
ATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCACCTAGGCA128880.1952519595643009No Hit
GAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTG128100.1940702670715933No Hit
ATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGC127080.19252497688882184No Hit
GGATAAGTAGGCTTCCCACTGAGCAGGGAGCTCCATGCAGTGCTCAATCC126360.1914341838186302No Hit
CAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAG125600.19028279113342791No Hit
GCTAATAACAGGGATGCCTGGGTGTGTCCACTGGCTAAGTGTCTGCCTTC125280.18979799421334276No Hit
CCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGG124240.18822240422306596No Hit
GATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACT122870.18614686740895137No Hit
GTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCT121270.1837228828085255No Hit
AGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTAAGAC116150.17596613208716286No Hit
CACTTAGCCAGTGGACACACCCAGGCATCCCTGTTATTAGCTATTTTTAA115550.17505713786200316No Hit
ATCCCAGGACGCAGGGGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCA115250.1746026407494233No Hit
CAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATT114080.17283010201036192No Hit
GTTAGTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGA113590.17208775672648152No Hit
GAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGC112620.17061821606247335No Hit
GTTTTAGTCAGCCATACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATT112550.17051216673620473No Hit
ATCCTAGTTTGAGAAAAACATGCACAACTGAATTTGAAGACGACAAACTG110430.1673003871406405No Hit
ACATTGAGCTCCCTGCTCAGTGGAGAGCCTCCTTCTCCCTCTTCTGTTTC109870.16645199253049145No Hit
ATCCCAGGGTCCTTCCTGGGATTGAGCCCCACATTGAGCTCCCTGCTCAG107310.16257361716981014No Hit
CCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTT107080.1622251693834989No Hit
AATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAAC106620.16152827381087648No Hit
GCTACATCTTGCCAGTCATTGAGTCCTTTTGATTCAACCTCTTAAGTGCT103640.15701360249258337No Hit
CCTCTTAAGTGCTTTTTGCATTCTATTCACTTTCTCTAATCCTACTACCA103040.15610460826742367No Hit
GGCTAAGTGTCTGCCTTCAGCTCAGTTCCTGACCCCTGCGTCCTGGGATT102960.15598340903740238No Hit
GTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACT102450.15521076394601666No Hit
TCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCATCTTTGA102420.15516531423475866No Hit
ATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAA99100.15013554618887506No Hit
GAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAGGGAGAAGGAGGCTCTC98410.14909020282994143No Hit
TCCCTGTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTAT98260.1488629542736515No Hit
CTGTCAAATAAAATCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCC97690.1479994097597498No Hit
CTATTCACTTTCTCTAATCCTACTACCACTATCTTATTATAGTTACTGTT97690.1479994097597498No Hit
GCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTTGGCTCAGGTCATGATC96920.14683286717079486No Hit
ACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGG96170.14569662438934525No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC95360.14446948218537967No Hit
TTATTTGACAGAGAGAGAGAAAGAGCGCACAAGCAGGGGAAACAGAAGAG95250.1443028332441004No Hit
GTTATTAGCTATTTTTAAAACCAATTTTATCTGTTTTATTTTTATGGTGC95080.14404528488030516No Hit
ACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCT95060.14401498507279983No Hit
GAGCAGGGAGCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAA94680.1434392887301987No Hit
AGATATTAATCATTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAA93660.1418939985474272No Hit
TTATTTCTTCCATTTGGTCCTCAGTTTGTCGTCTTCAAATTCAGTTGTGC93480.14162130027987932No Hit
GGTCAGGAACTGAGCTGAAGGCAGACACTTAGCCAGTGGACACACCCAGG91990.13936396462073275No Hit
CTTCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTT91970.13933366481322745No Hit
CTCAATGACTGGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGA91250.13824287174303582No Hit
GAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCA90830.13760657578542404No Hit
TAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACTAAAA90750.13748537655540274No Hit
CCACAGAAGTCTTACCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTG90520.13713692876909153No Hit
CATTGAGTCCTTTTGATTCAACCTCTTAAGTGCTTTTTGCATTCTATTCA89690.13587948675762063No Hit
CCCTTATTTGTCCTCTGCTACATCTTGCCAGTCATTGAGTCCTTTTGATT88990.1348189934949343No Hit
CAAATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGT87790.13300100504461496No Hit
CAGTTTGTCGTCTTCAAATTCAGTTGTGCATGTTTTTCTCAAACTAGGAT87510.13257680773954042No Hit
GGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAA86530.1310921171717796No Hit
ACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAG86060.1303800716954045No Hit
GAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGA84960.12871358228261176No Hit
GGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTAGGATTAGAGA84870.12857723314883782No Hit
AGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAGTA84360.12780458805745207No Hit
GTGTCTGCCTTTGGCTCAGGTCATGATCCCAGGGTCCTTCCTGGGATTGA82220.12456250865438252No Hit
GGATTAATCTTGGGGTGCCTAGGTGGCTTGGTCGGTTAGTGTCTGCCTTT81720.12380501346674945No Hit
GGCAAGATGTAGCAGAGGACAAATAAGGGAAAATCAAAGATGAAAAGGTA80890.12254757145527855No Hit
GCTCCATGCAGTGCTCAATCCCAGGACGCAGGGGTCAGGAACTGAGCTGA80400.12180522617139813No Hit
ACTGTGAGGAAAGAAGAAAGAACAAAAGATTCATTATTTCAAGATTTTAT80310.12166887703762418No Hit
CTTCTGTTTCCCCTGCTTGTGCGCTCTTTCTCTCTCTCTGTCAAATAAAA79750.12082048242747513No Hit
AACTAGGATGCCTAAAATGGATTAATCTTGGGGTGCCTAGGTGGCTTGGT78850.11945699108973562No Hit
AACTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCA78560.11901764388090842No Hit
AAAGTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTC78540.1189873440734031No Hit
CCCTGGGATCATGACCTGAGCCAAAGGCAGACACTAACCGACCAAGCCAC78350.11869949590210253No Hit
GAACAAAAGATTCATTATTTCAAGATTTTATTTGACAGAGAGAGAGAAAG77650.11763900263941623No Hit
AAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGGGATGCC77200.11695725697054647No Hit
GGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAGCAGGGAG76290.11557861572905428No Hit
GCTCAATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAG75940.11504836909771111No Hit
GAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGA75080.11374547737498225No Hit
AGCGAAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTA74910.113487929011187No Hit
CCTTTTCATCTTTGATTTTCCCTTATTTGTCCTCTGCTACATCTTGCCAG74060.11220018719221077No Hit
AACATGCACAACTGAATTTGAAGACGACAAACTGAGGACCAAATGGAAGA73120.11077609623946058No Hit
ATGTGGGGCTCAATCCCAGGAAGGACCCTGGGATCATGACCTGAGCCAAA72370.10963985345801097No Hit
AAAGCAGGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAAT72150.10930655557545242No Hit
GTGAATAGAATGCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAAT71650.10854906038781935No Hit
GGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAAAAGCACTTAAGAGGT71150.10779156520018629No Hit
GAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATAGTGGTAG70940.10747341722138039No Hit
GAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCTGACT70810.1072764684725958No Hit
GATGAAAAGGTAAGACTTCTGTGGTTTTAGTCAGCCATACTGTGAGGAAA70250.10642807386244675No Hit
GGTTTTTGTGAATGCTTTATCTAGATATTAATCATTATTTCTTCCATTTG70010.10606447617238286No Hit
GGCAGACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATT69490.10527668117724448No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC69260.10492823339093325No Hit
CCATAAAAATAAAACAGATAAAATTGGTTTTAAAAATAGCTAATAACAGG69120.10471613473839601No Hit
AAAGCAGGGAGAGAGCAGCAGGCAGCAGGATAAGTAGGCTTCCCACTGAG69090.10467068502713803No Hit
AATAAGGGAAAATCAAAGATGAAAAGGTAAGACTTCTGTGGTTTTAGTCA68800.10423133781831083No Hit
GGCTACTGAAGAAACAACAGGGGAGAGGTAACAGTAACTATAATAAGATA68660.10401923916577359No Hit
CTCTAATCCTACTACCACTATCTTATTATAGTTACTGTTACCTCTCCCCT68060.1031102449406139No Hit
GCTCTCCACTGAGCAGGGAGCTCAATGTGGGGCTCAATCCCAGGAAGGAC67300.10195885225541161No Hit
TCTTGAAATAATGAATCTTTTGTTCTTTCTTCTTTCCTCACAGTATGGCT66860.10129225649029452No Hit
CCTAGGCACCCCAAGATTAATCCATTTTAGGCATCCTAGTTTGAGAAAAA66540.10080745957020934No Hit
GCAAAAAGCACTTAAGAGGTTGAATCAAAAGGACTCAATGACTGGCAAGA66520.10077715976270403No Hit
GACACTAACCGACCAAGCCACCTAGGCACCCCAAGATTAATCCATTTTAG66490.10073171005144604No Hit
TCTTTCCTCACAGTATGGCTGACTAAAACCACAGAAGTCTTACCTTTTCA66070.10009541409383427No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)