FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4016285
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTA379540.945002657928907No Hit
CCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAG275810.6867291539320541No Hit
CCCCCACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAG263840.6569254920903272No Hit
ATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGA260640.648957930027376No Hit
GCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAA256610.6389237815543468No Hit
AAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCT234410.5836488197426228No Hit
GGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATG215130.5356442583133417No Hit
GTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGG207830.5174682573572345No Hit
GGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGAT199360.4963791165218604No Hit
GTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTAC197810.49251982864761834No Hit
TCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAG196360.48890952708784363No Hit
GTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTT183950.458010325462461No Hit
GGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTC183330.45646661031276414No Hit
CTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCA182520.45444982116557964No Hit
GAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCT181840.4527567142272025No Hit
CTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCA176500.4394608450346527No Hit
AGCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGC165650.4124458299149587No Hit
GATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGT158740.3952408755852735No Hit
GGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAATGCTGTT144730.3603578929284152No Hit
AATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACA140680.35027394719249255No Hit
AGCGAAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGC124490.3099630628802488No Hit
CAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTG124270.3094152929884209No Hit
CTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGGCTTCAGAG121460.30241877755189184No Hit
GGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGC109990.27386004728250113No Hit
GTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTG107170.26683863321452533No Hit
CTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACAC106070.2640997837553859No Hit
GCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTTTG104300.25969272598931603No Hit
CTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTAT100020.24903611173011875No Hit
GTAGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAAC98870.24617276911374567No Hit
AGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCA97400.24251267029107743No Hit
GGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCA95810.23855378789104856No Hit
AAAGCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAG95050.23666149190109767No Hit
AGCAAACACTCTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCT93940.23389774381051145No Hit
CCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACA93700.23330017665579011No Hit
TGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAA93530.2328768999211958No Hit
GTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCC92930.23138298203439245No Hit
GTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAA87550.21798751831605573No Hit
GCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAA86770.2160454250632114No Hit
GGTAAATATATTTTTAAAAACTTTACTCTGGGGCGCCTGGGTGGCTCAGT86150.2145017099135146No Hit
GGTTCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTA85130.2119620495059489No Hit
CTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTA85040.21173796182292842No Hit
ACCCCAGGCCATGTTAGGTTTAATCAGGACCTGGTTTTCAGTGAGATGAA82600.2056626957499281No Hit
GCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGA81300.2024258736618542No Hit
GGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGC80930.20150462429832544No Hit
TCCCTGAACCTTCATCTCACTGAAAACCAGGTCCTGATTAAACCTAACAT79880.1988902679964196No Hit
CAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCATTTT79780.19864128168195233No Hit
GCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAACAGCAT78190.19468239928192346No Hit
TCAGGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTT77410.1927403060290791No Hit
CAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGAGGAAGCAGGCTCCCT77280.19241662382027172No Hit
TTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAG75960.18913000446930434No Hit
GGGAAGTGATTGACAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAG75930.1890553085749642No Hit
CTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAG75360.187636086582501No Hit
GTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAG74570.1856690946982099No Hit
ACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGG74400.18524581796361564No Hit
CCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCT73590.1832290288164311No Hit
GGGTGGGGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTT72670.18093835472333264No Hit
GGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAAAAAT70420.17533616264782007No Hit
CTGTGGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTC70120.17458920370441838No Hit
ATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAA67170.16724410742763524No Hit
GTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGA66300.1650779264917704No Hit
TTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGCTGCTTCCTGTCCCTCTGC63740.1587038768414094No Hit
CTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGA63280.15755853979486018No Hit
CAAAAATGCTGTTTCTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGC60980.15183185456211398No Hit
GACAGAGAGAGACCACAAGTAGGCAGAGAGGCAGGCAGAGAGAGAGAGGA60080.14959097773190896No Hit
TCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTTAACCTA59580.14834604615957284No Hit
GGACAGGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTT59480.14809705984510563No Hit
GTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGCCACAGAGGG59360.14779827626774494No Hit
CCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCCCAGCAGGA57820.1439638870249497No Hit
GGGAATGCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTC57750.14378959660482263No Hit
GGAAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATC56820.14147402388027744No Hit
GGCTATCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGA56710.14120013893436348No Hit
TAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGG55560.1383367963179904No Hit
GCCTGGGGGCTCAGTAGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATC53950.13432811665506805No Hit
CTCCAGAGTGTTTGCTACTTGAGGACCGACTCCTGCTGGGCATTGATAGC53630.13353136044877292No Hit
GTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTCAGCTGGGAGTCTGC53500.13320767823996554No Hit
GTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCTC53160.132361124770777No Hit
GGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTTAGGCTCCCTGCT51540.12832754647640793No Hit
ATCAGGACCTGGTTTTCAGTGAGATGAAGGTTCAGGGAAGTGATTGACAA51540.12832754647640793No Hit
ACTTTACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTC51310.1277548779531333No Hit
ACTCTGGGGCGCCTGGGTGGCTCAGTGGGTTATGCCGCTGCCTTCGGCTC51170.12740629711287918No Hit
GGCAGAGGGAGAGGGGGAAGCAGGCTTCGGGCTGAGCAAGGAGCCGGATA50640.12608666964620288No Hit
GTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGG50440.12558869701726844No Hit
GGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCACTGA50010.12451805586505937No Hit
GCAGGGGGAGTAGCAGAGGGACAGGAAGCAGCAGACTCCCAGCTGAGCAG49300.12275025303234208No Hit
GTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAATGCC47910.11928934326124764No Hit
GCGAAAGCAGGACTCTAAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCC47890.11923954599835421No Hit
GCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCCCTTGTT47100.11727255411406311No Hit
TCAATGCCCAGCAGGAGTCGGTCCTCAAGTAGCAAACACTCTGGAGAAAC46680.11622681159330077No Hit
CTGGAGAAACAGCATTTTTGTCAATCACTTCCCTGAACCTTCATCTCACT45510.11331367171403424No Hit
AGGTTAAGTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGAT45410.11306468539956702No Hit
GTTCCATCCCAGGACCCGGAGATCAAGACCTGAACTGAAGGCAGACACTT45200.11254181413918585No Hit
GCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAA45120.11234262508761206No Hit
GAGCCTAACAAGGGGTTCCATCCCAGGACCCGGAGATCAAGACCTGAACT44900.11179485519578415No Hit
GTGTCTGCCTTCAGTTCAGGTCTTGATCTCCGGGTCCTGGGATGGAACCC43860.10920539752532503No Hit
AAGCAGCAGACTCCCAGCTGAGCAGGGAGCCTAACAAGGGGTTCCATCCC43480.10825924953034956No Hit
GGTCATGATCTCAGGGTCCTGGGATCGAGTCCCGCATCGGGCTCTTTGCT43420.10810985774166923No Hit
AAAGTGACTTTCCTGACGGGTTAGCTCTGAAGCCCTCTGTGGCTATCAAT41270.10275665198062388No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)