FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1367340
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAG168771.2342943232846257No Hit
ACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCA110840.8106250091418375No Hit
CCCTTATTTCTCTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAA79890.5842731142217737No Hit
TCTCAGAAGAGTATCAAGAACAATTCAGAACATACAATATGTGTGTGATA78360.5730835051998772No Hit
GGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAA71560.5233519095470037No Hit
GTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTA62570.45760381470592537No Hit
CTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTA61410.4491201895651411No Hit
ACTTATAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTT60730.4441470299998538No Hit
ATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTG56510.4132841868152764No Hit
GTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTA56380.4123334357219126No Hit
TAACTAAACCACCTGAATCACCTATGATTTGAGGTACTACGTGTTAGGAA54170.39617066713472876No Hit
GGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCT51700.37810639636081733No Hit
ATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTT50290.36779440373279504No Hit
CAAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTC45370.33181213158395134No Hit
ATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGG43900.3210613307589919No Hit
ATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAGAG43290.3166001140901312No Hit
CAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAAGAG42740.31257770561820764No Hit
ATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCATAATCA35130.2569221993066831No Hit
CTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGA33790.2471221495750874No Hit
CCTATGATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAA33000.24134450831541535No Hit
GTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAAATCATAG31930.23351909547003669No Hit
ACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTG30970.22649816431904282No Hit
ATTAAGGAAGTTTGGGTTTTTTTAAGAGTACTTCTGTGGGAACTGAGTAA28140.20580104436350874No Hit
TACTAATACAGACCAGGGAAAGAAGGAGGAGGGAGAGGGGATGAGAAGAG28090.20543537086606112No Hit
CTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAG25640.18751736949112877No Hit
AAATAACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCC25530.18671288779674405No Hit
GATTATAAAAGGTCACCAAAAAGGACAAAAAACATTAAAAAGCAATGTCC25270.18481138561001653No Hit
CATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACCTGAATCAC23840.17435312358301522No Hit
ATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCAGT23430.1713546009039449No Hit
AAATAGTCCTTACTTATAACTAAACCACCTGAATCACCTATGATTTGAGG22580.1651381514473357No Hit
GTTTTTAAGTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCT21860.1598724530840903No Hit
ATATAAAACAGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTGTATACACAC21110.1543873506223763No Hit
TCCTAACACGTAGTACCTCAAATCATAGGTGATTCAGGTGGTTTAGTTAT21010.1536560036274811No Hit
GTATCAAGAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACT20100.14700074597393478No Hit
TTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACCACC19850.14517237848669678No Hit
TATATAACCAGAACTAAAAATCAGGTATCATTCTTGTCCCATTTACTAAA19790.14473357028975967No Hit
TTATTAACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACT19580.14319774160047977No Hit
GTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGC19330.14136937411324177No Hit
ACTTTATGCTTTAAGAAAGTTTTGGGAAGTTTCTGATAAAATATTGGTTC19170.14019921892140946No Hit
GTGTACAGTTGAGTGATTTTTAATCAGTTTGAGTATGCAGCTATCATCAC18860.13793204323723432No Hit
TTCTAGGACATTGCTTTTTAATGTTTTTTGTCCTTTTTGGTGACCTTTTA18850.13785890853774482No Hit
CCATTAACTGGATATGATTGCATAATTAAGGAAGTTTGGGTTTTTTTAAG18280.13369023066684219No Hit
CATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAA18200.13310515307092605No Hit
GTGTTAGGAAATAAAGGCTCATCTTGAGAACTGATACCTTAGCCCCTTGA18080.13222753667705178No Hit
GAAATAAGGGAGGACCTCTCTTTGCCTCCAAATATTCATCCAGTAGGTGG17840.1304723038893033No Hit
GTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGACTCA17410.12732751181125396No Hit
GAACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGG17370.1270349730132959No Hit
GTATTATATAGGATTTGCTAAATGACCCAGAATGAGAGGAGATAAATCAT17230.1260110872204426No Hit
ACACTGTATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAG16950.12396331563473606No Hit
GAATGAAGGGTGACTGCAAATGAGCACAAAGATCATTCTGGGATGATGAA16520.12081852355668671No Hit
CTGTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTT16360.1196483683648544No Hit
TCTCTAATGTATCAGCCACTGAGTCATCCTTCACATTGGAATTACTGATT16270.11899015606944871No Hit
GATTTGAGGTACTACGTGTTAGGAAATAAAGGCTCATCTTGAGAACTGAT15880.11613790278935743No Hit
GGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGT15810.1156259598929308No Hit
ACATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCA15430.11284684131232904No Hit
ATTTTACTCAGTTCCCACAGAAGTACTCTTAAAAAAACCCAAACTTCCTT15400.1126274372138605No Hit
GGTATCAGTTCTCAAGATGAGCCTTTATTTCCTAACACGTAGTACCTCAA15070.11021399213070635No Hit
AATATTAACAGCCTGAAAAGGATTCTTGATTACTACCATTCATCAACTAT14900.10897070223938449No Hit
CTTGTAAACCTGGAATAATGTTCCATTAACTGGATATGATTGCATAATTA14790.10816622054499979No Hit
ATTCTGGGATGATGAAAATGTTCTAAAAATAAACTGTGATGATAGCTGCA14590.10670352655520939No Hit
TATTTATACCTGTCTTTCCACATACTTTTTTCAAATGGTTTCATGAAAAG14450.1056796407623561No Hit
AACCAGGATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAA14390.105240832565419No Hit
GAACAATTCAGAACATACAATATGTGTGTGATAACCACCTACTGGATGAA14380.10516769786592947No Hit
AATGTATATAATTCCAAACTTCAATCAGTAATTCCAATGTGAAGGATGAC14220.10399754267409717No Hit
CTTGAGAACTGATACCTTAGCCCCTTGATGATGATTACAGAGAAATAAGG14220.10399754267409717No Hit
ATATTACGTAGTGTGTATACACACACACACACACACACACACACACACAC14130.10333933037869147No Hit
GTAATCATCATCAAGGGGCTAAGGTATCAGTTCTCAAGATGAGCCTTTAT13930.10187663638890108No Hit
ATGATAAACTTGAGTTACACTGAATTTTACTCAGTTCCCACAGAAGTACT13900.10165723229043251No Hit
ATATTACTCTACATCTAGATGATCAAATAGTCCTTACTTATAACTAAACC13880.10151096289145348No Hit
ATACAATATGTGTGTGATAACCACCTACTGGATGAATATTTGGAGGCAAA13800.10092588529553732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)