FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G5a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G5a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences951528
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC19240.2022010912973659No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT18220.19148149082318125No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT15890.16699456032822996No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG15870.1667843720836381No Hit
GTGTATTGAAGCATTAGACAGATAAAGCCAGTAGCCCAAGGTCACACAAT15340.16121438360195392No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT13980.14692158296970767No Hit
TACCAGAAGTGCTGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATA13560.1425076298332787No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG9920.10425336931756081No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG9870.10372789870608118No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT9830.10330752221689744No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG9620.10110054564868295No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)