FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3998504
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT133160.33302455118214214No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT120280.3008125038764498No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA108570.27152655093004785No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT79540.19892439772474907No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG73140.18291841148589572No Hit
CTTCTAGGTATTTATTTCTTCTCTCATCAAAAGCAGAAAGCACCATCCTC71630.17914199910766626No Hit
CTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGG68700.17181425853269122No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT63960.15995982497454048No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA63910.15983477820704942No Hit
TAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAA59400.14855555977935747No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA57250.1431785487772427No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT57000.14255331493978748No Hit
GTATTGGAGTCCATGGTTTCCACATTCTCATTTGAAGCAATCTGGACCCC56800.14205312786982333No Hit
GAGCAAAAGCAGGGTAGATAATCACTCAATGAGTGACATCGAAGCCATGG54320.13585080820226764No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT51960.1299486007766905No Hit
AACTCATCCTTTATGACAAAGAAGAAATAAGGAGAGTTTGGCGCCAAGCA49910.12482168330955777No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC48320.12084519610334266No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA48320.12084519610334266No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA48300.12079517739634624No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT47800.11954470972143581No Hit
TCCCAGCACTGGGATGCTCTTCTAGGTATTTATTTCTTCTCTCATCAAAA46250.11566825992921352No Hit
GTCATACTCCTCTGCATTGTCTCCGAAGAAATAAGACCCTTCATTACTCA46190.11551820380822427No Hit
GCATTAGAGAGCACATTCTGGGATCCATTCCGGTGCGAACAAGCGCTCTT45370.1134674368213712No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT45070.11271715621642493No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA44520.11134164177402349No Hit
CTTTCCGTCTACTCTTCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCT44340.11089147341105574No Hit
TCATTACTCATGTCAAAGGAAGGCACGATCGGGTTCGTTGCCTTTTCGTC44150.11041629569458979No Hit
GTCTTACTCATATCATGATTTGGCATTCCAACCTGAATGATGCCACATAT43790.10951595896865426No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG43750.10941592155466143No Hit
CAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATAC43570.10896575319169369No Hit
CTCCAATACCCTGGAACTGAGAAGCAGATACTGGGCCATAAGGACCAGGA43350.10841554741473311No Hit
CTCTAATGCAAGGTTCAACACTTCCCAGAAGGTCTGGTGCCGCAGGTGCT42390.10601464947890511No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)