FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5271146
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCTAATACAGTTCCGATCTTATTAGTTAAGACAGAATCTGTCTGTTCCA156150.2962353916966064No Hit
GTTCAATGAAATGTTCAAATAAGGAAAGATGAAGTTCATATGGTGCATGG153360.290942425043814No Hit
ATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGACCTC149780.2841507330663958No Hit
TCATTGAACTGCTCACTGAGTCCAGTGAAGCTTCAAAGAATGCCAAATTA146850.27859216952063176No Hit
GTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAGAAATTTGTAG137880.2615749971638046No Hit
GTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTA136130.25825503600165883No Hit
CCATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCAC121540.2305760455126836No Hit
TCACTGGACTCAGTGAGCAGTTCAATGAAATGTTCAAATAAGGAAAGATG113580.21547496502658056No Hit
GTCCAGTGAAGCTTCAAAGAATGCCAAATTAATGAGGGAATTCCAGCTAA108050.20498388775419996No Hit
CTTTAGATGTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTC104380.19802145491701426No Hit
GCATACATACGGCCTGAAAGCTTCCAACATAATTTGGTCCTAGTTCATAA102950.19530857236737514No Hit
TCCATAAACATCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTC101450.1924628913712502No Hit
GAAATAAACAATGAAGAGAAGCTTGATACTGGAACTGAAGAAGAGTTTGG100460.19058474191380773No Hit
CTGTTGATAGTGGGCATGAGACCTCCATTATTCCAAATTCAACTGCTTTC100420.1905088570872444No Hit
GTCTAAGGGAAAGCCGTGACCCCGGGGTCCAGCAGGTGCCTGCCCTGTGC98230.186354162832902No Hit
GTTTATTTCCATAACTACAAATTTCTCACAAACTGCAAACGTTGGTAAAG97590.18514000560788868No Hit
ATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGCTTCTCTTCA96550.18316700011724205No Hit
CTTCTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAG94010.1783483136304705No Hit
ACTCATAGCAGCAATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCA92240.17499041005504307No Hit
CCATTATTCCAAATTCAACTGCTTTCCAGGATCTACTTTGTGATTTTGAA89390.16958361616240566No Hit
CTATCAACAGTTCCCACCAAGGAAAATGTTAGATGGAGAATGTGGCTGTT88000.1669466184393299No Hit
GTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTTAGATGTGTAA86640.16436653433617662No Hit
TATGTATGCCGTGTAAAGATACAAAGTCTGAAGGCGTAGTCCCATCTCCT85280.16178645023302332No Hit
GAATAAGCCTGGCCTCAGTTTTTCTGACGCGACAGAGGTTTTGGGCACTC84280.15988932956894003No Hit
GGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGGGATTAGCTGGA78250.1484496919645178No Hit
GTCTATGCGTACATTGGCACTCCACCTGCCCAGCGTCAAATTGCTTCCTT77050.1461731471676178No Hit
ATCTTATACTTTTGAGGAATAGACTTCTAGATATCTTGCTAAAACTAGTT76030.14423808409025288No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT75670.14355512065118287No Hit
GTCCTGACCAGTTCTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTT74330.14101297896131126No Hit
GTGTAAACTAGTTTTAGCAAGATATCTAGAAGTCTATTCCTCAAAAGTAT74330.14101297896131126No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT74190.14074738206833962No Hit
TTACCAACGTTTGCAGTTTGTGAGAAATTTGTAGTTATGGAAATAAACAA73510.13945734001676297No Hit
GTATCAAGCTTCTCTTCATTGTTTATTTCCATAACTACAAATTTCTCACA71480.13560618506867386No Hit
GCACGGGAGAATCTCATAAAAAGAAGATTCTTCACTAAAATCATCAACAT71190.13505602007608972No Hit
GTCTTGTATCTGCTAATCTTATACTTTTGAGGAATAGACTTCTAGATATC71000.1346955671499139No Hit
ACTATAAGTTCTCCACATCGAAAGCGGGCACAGCACGGGAGAATCTCATA69480.13181194374050728No Hit
ATATGAACTTCATCTTTCCTTATTTGAACATTTCATTGAACTGCTCACTG67170.12742959500647488No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA67010.12712605570022154No Hit
AATTAATGCTTGTCTTTTCTTTAGATGTGTAAACTAGTTTTAGCAAGATA66910.12693634363381323No Hit
TCATAATCCAGTCAAAACCCAGTGTCCTGACCAGTTCTTCACAAGCTTGC66570.1262913226080249No Hit
CATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAGAACTGGTC66360.1258929272685674No Hit
CGCATAGACTGTGCTGACCACAGGTGGGTTAGCGGAACCTGAGCCACCAG64950.12321798713220997No Hit
CTCCTTAACAGCCACATTCTCCATCTAACATTTTCCTTGGTGGGAACTGT64580.12251605248649915No Hit
GCTCAGGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTT64000.12141572250133083No Hit
GTTCATATGGTGCATGGAGCCAAACTTCAAAATCACAAAGTAGATCCTGG63920.12126395284820417No Hit
GATATCTAGAAGTCTATTCCTCAAAAGTATAAGATTAGCAGATACAAGAC63050.1196134578704517No Hit
CTAGATATCTTGCTAAAACTAGTTTACACATCTAAAGAAAAGACAAGCAT63020.1195565442505292No Hit
AGTTTACACATCTAAAGAAAAGACAAGCATTAATTTGCAAGCTTGTGAAG62750.11904432167122671No Hit
GGATAGACTGATTACTCAGTAGCACAACAAGAATCCTCATGGCTGCCGTA62460.11849415667864256No Hit
AACCTGAGCAGATCATTGCTGTTGGGAAAACCTTGTAGTAAGAAGCTCAG61950.11752662513996007No Hit
GATTAGCAGATACAAGACCTCCAAACTCTTCTTCAGTTCCAGTATCAAGC59460.11280279468639266No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG59450.11278382347975184No Hit
GAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAATGATC58820.11158863746137937No Hit
GCTATGAGTAATGTGCTGAGCTTCTTACTACAAGGTTTTCCCAACAGCAA57590.10925517904455692No Hit
GTTTTGGAGTAGTTCCTCTTTGGTGGAAACAATCAGAGATCGATCTTTTG57030.10819279147267027No Hit
TCTTACTACAAGGTTTTCCCAACAGCAATGATCTGCTCAGGTTTGGCCAG56940.10802205061290278No Hit
GAAGAAGAGTTTGGAGGTCTTGTATCTGCTAATCTTATACTTTTGAGGAA56810.10777542492657194No Hit
CCTCAAAAGTATAAGATTAGCAGATACAAGACCTCCAAACTCTTCTTCAG56330.10686480700781197No Hit
GTATTAGGATTCAATGTGGGCAGGAGTGCTGGCGGGAGATCGACGGTCAG56270.10675097976796696No Hit
GGTTTGGCCAGTTTATTTCTTCTACTTTACCAACGTTTGCAGTTTGTGAG55550.10538505288982698No Hit
ATCTAACATTTTCCTTGGTGGGAACTGTTGATAGTGGGCATGAGACCTCC54710.10379147153199701No Hit
GCATTACGTGTGCCTTGCCATTGTCTTATCCGCAAAAGATCGATCTCTGA54530.10344998981246203No Hit
GAAATAAACTGGCCAAACCTGAGCAGATCATTGCTGTTGGGAAAACCTTG54300.10301365205972288No Hit
ATGGTAGGTTGGGATAAAGACATATCTCGAAGAGTCAGTAGCAATTTGGG53720.10191332207455457No Hit
CTTCACAAGCTTGCAAATTAATGCTTGTCTTTTCTTTAGATGTGTAAACT52940.10043356795656959No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)