FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G1a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_G1a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1740248
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGACATCTTAACAATATTGTTCTTCCAATCCATGAGCATGGATTGT107370.6169810279914127No Hit
GGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGA106290.6107750159747347No Hit
AACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCC71350.409999034620353No Hit
CCCTATGCCATTGGGCAAGGGGCATTAGCCTGCAGAAAGGATCTTGCTGT68350.39276011235180275No Hit
AGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGA65930.3788540483885055No Hit
AGCAGGGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCC63970.3675912858397194No Hit
AAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGC62660.3600636231157858No Hit
TAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAG62280.3578800262951028No Hit
CCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCC60380.3469620421916876No Hit
GTCCTGGGTGGCAGTCCTATCGGGGCACCATAGGGGCAATTGCAGCCTGT58920.3385724333543265No Hit
TCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGGCA56670.32564324165291386No Hit
GTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGC54440.3128289760999582No Hit
CAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTT51850.29794603987477647No Hit
ATGTAAAGAAGCTTAAAACCACTTACCTAAAGTCTTGCTTTATAATCAGT50580.29064822944775687No Hit
ACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCA48430.2782936684886292No Hit
ATGTAATACCTATCAAAATACCAATAACACTTCTCACAGAACTAGAACAA45540.2616868400365925No Hit
AATCTATACACTGAAAACTAGAAAACACTGATGAAAGAAATTGAAGATGG45200.2597330955128234No Hit
CTCCCTGCCCAGTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCC44830.25760696176636894No Hit
GAACTAGAACAAATAATCCTAAAATTTGTATGGAACCACAAAAGAGCCTG44180.2538718619415164No Hit
AAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAGCAATAAGGACAC41450.23818444267713568No Hit
GCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAACT41390.23783966423176467No Hit
GCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTC40150.23071424302743057No Hit
GCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCA39540.227208995499492No Hit
TAGTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGA38380.22054327888898595No Hit
GAATAAAACTGTCAAAAAATAAAGAAAGTGACTTAGAGCAGGATCCATAA37330.21450965609499337No Hit
TTTCTGATGAGAGTGAGCACTGGTTTCACTGGTTTTTCATGATGTAAAGA37140.21341785768465185No Hit
GTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTG36430.20933797941442828No Hit
CTGTATAACTATACTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTA34640.19905208912752667No Hit
ACTTAGAGCAGGATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAAC32810.18853634654371101No Hit
CCCCTATGGTGCCCCGATAGGACTGCCACCCAGGACTGTGGCCCATAATC32650.187616937356055No Hit
AGTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTA32550.18704230661377No Hit
ATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTT31800.18273257604663243No Hit
AGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAAC31780.18261764989817542No Hit
GCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAAGAA31590.1815258514878339No Hit
CCATAGGGGCAATTGCAGCCTGTGGATGAGGATGGGAAGAGGACTGGGCA31570.18141092533937692No Hit
CCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTTCAACAAGCAG31400.1804340530774924No Hit
GGAAACAAGTACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAA31340.1800892746321214No Hit
ACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTT31290.17980195926097892No Hit
CTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTGATGAGAGT31080.1785952347021804No Hit
TCTTTACATCATGAAAAACCAGTGAAACCAGTGCTCACTCTCATCAGAAA30640.17606685943612635No Hit
GAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTCCCATC28650.1646317076646547No Hit
GTATTTCTTTCTCAGTGTCATGAATTGTGGTTAATGCTTGCTAGAAAAGC27760.1595174940583181No Hit
GTCCTCTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCC27390.1573913603118636No Hit
GTACAACTTTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATT27260.1566443403468931No Hit
GTATGTTTGTTTTGACTCCTTTTAAAAAGAGTAGAGCATAAACTATCTGG27130.15589732038192258No Hit
ACCCAGGACTGTGGCCCATAATCCCAGCTTTTACTATGCCTATGGCTCCA27110.15578239423346557No Hit
ACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTAT26520.152392072853984No Hit
GTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAACTTGAAGTCTGG26390.1516450528890135No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT26030.1495763822167875No Hit
CTTCCCATCCTCATCCACAGGCTGCAATTGCCCCTATGGTGCCCCGATAG25980.149289066845645No Hit
GTAACAAATTGCTACCTTCAACAAGCAGAGCTAGACTCGGTTTGCAGTTC25950.1491166776229595No Hit
GACTTAATATGTTGCAGAGAAGGTAGTGTTAATGAACCGAAGTTAGAGGT25920.148944288400274No Hit
GTTCCAGTCACCCCCAAAATGCATAAAGCCAAGTAACAAATTGCTACCTT25780.148139805361075No Hit
GAGCTAGACTCGGTTTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCT25460.14630098698576294No Hit
ACTCTGGACCAAAAATAAAACTTCTTTGAAGGAGACAGAATCCTTTTTAG25150.1445196316846794No Hit
TTGTAGTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCC25060.14400246401662292No Hit
GAGTAGAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATG24710.1419912564186254No Hit
GTTTATATGAGAAAGGAGGGGAATGAAAGAGAAAATAGAATCAAAACACC24140.13871586118760085No Hit
CTAGAAAACACTGATGAAAGAAATTGAAGATGGCACAAACAAACGGGAAG24100.13848600889068685No Hit
TAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGG24050.13819869351954434No Hit
AGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAACTTCTT23520.1351531505854338No Hit
GGTTAGGCCTTTAAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACT23470.1348658352142913No Hit
GGTCTGAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAA23430.1346359829173773No Hit
GGACAGAGCTGAGAAACTGATCTCTGCTCTCTCCCTGCCCAGTCCTCTTC22920.13170536613172376No Hit
GGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCAAAAATAAAAC22570.1296941585337262No Hit
ATCAAAATACCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAA22490.12923445393989821No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA22430.1288896754945272No Hit
GTAGAAACAAGGGAGTTTGGAGCCATAGGCATAGTAAAAGCTGGGATTAT22370.12854489704915623No Hit
CCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATTTG22260.1279128032326427No Hit
GGCATAGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTA22100.1269933940449867No Hit
ATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTC21940.12607398485733068No Hit
GTCATGAATTGTGGTTAATGCTTGCTAGAAAAGCGTGGAATACTTGAACT21660.12446501877893265No Hit
GAGAAACGCAGGTGCCTAAGTTCCAGTCACCCCCAAAATGCATAAAGCCA21660.12446501877893265No Hit
TTATAAAGCAAGACTTTAGGTAAGTGGTTTTAAGCTTCTTTACATCATGA21540.12377546188819064No Hit
TTGCAGTTCTATCCTAAAACTCCTTTTCACCAAGCTTAGCTTAAAGGCCT21390.12291351577476313No Hit
CTCTTAATCTCTGAAACTGTACGCAAATTTGCATAATGTGCATTTTTCTG21260.12216649580979262No Hit
GAGCATAAACTATCTGGTGTTTTTAAAATCTGCAATATTGAGATGGAGAA20950.12038514050870909No Hit
CCACAAAAGAGCCTGAATAGCCAAACAACCTTAAAAAAGGAAAGCAAATC20830.11969558361796709No Hit
ATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCTTCAAA20630.11854632213339708No Hit
CTTAATGACTGAGTCATGAAGGCACCCCTTAGTACAACTTTGTAGTATAA20500.11779930216842657No Hit
CAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATGG20100.11550077919928654No Hit
CTGTTAGAGTGAAGTTCAAGTATTCCACGCTTTTCTAGCAAGCATTAACC19970.11475375923431604No Hit
TAACAATATTGTTCTTCCAATCCATGAGCATGGATTGTCTTCCCGTTTGT19810.11383435004666002No Hit
AGCGAAAGCAGGGCTAGCTGGACGAGTCAGGAAACAAGTACTCTGGACCA19770.113604497749746No Hit
AAGCTAAGCTTGGTGAAAAGGAGTTTTAGGATAGAACTGCAAACCGAGTC19560.1123977731909475No Hit
TCCTAGTGATTAGGAAGTTCAGACTGTATTTCTTTCTCAGTGTCATGAAT19460.11182314244866248No Hit
GTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGGG19400.1114783640032915No Hit
CCAATAACACTTCTCACAGAACTAGAACAAATAATCCTAAAATTTGTATG19150.11004178714757895No Hit
AATTTGCATAATGTGCATTTTTCTGATGAGAGTGAGCACTGGTTTCACTG19150.11004178714757895No Hit
GCTAAAAAGGATTCTGTCTCCTTCAAAGAAGTTTTATTTTTGGTCCAGAG19120.10986939792489347No Hit
ATCCATAAATGGGCTTCTGGTCTCTTAATCTCTGAAACTGTACGCAAATT18890.10854774721763795No Hit
TCCCTGAAGTGATCAAAAACTTTATCCAGTATTTCCACAAAACCGTCTCA18740.10768580110421043No Hit
AGTAAAAGCTGGGATTATGGGCCACAGTCCTGGGTGGCAGTCCTATCGGG18640.10711117036192543No Hit
GCCCAATGGCATAGGGAGTGTCCTTATTGCTAAAAAGGATTCTGTCTCCT18630.10705370728769693No Hit
GTATAACTTGAAGTCTGGAACTTGATACCTCCAGATTTGCTTTCCTTTTT18550.10659400269386891No Hit
CTCCCTATGCCATTGGGCAAGGGGCATTAGCCTGCAGAAAGGATCTTGCT18420.1058469827288984No Hit
AGATATCCCTTATAGGAAAAACCTCTAACTTCGGTTCATTAACACTACCT18180.10446786894741439No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT18130.1041805535762719No Hit
GCAATATTGAGATGGAGAACTGATTATAAAGCAAGACTTTAGGTAAGTGG18080.10389323820512938No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT17800.10228427212673136No Hit
GTATTACATCAAATATGTAGATTTTGGGTAGTATAGACATCTTAACAATA17740.10193949368136035No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)