FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3922585
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAACTGAGAGCCACTTCTATCTAGAAGTCATTTTCTGTAACGAAC198320.5055849650166918No Hit
ATTTAAGAGTAACCATTTAACTACAAGATATGCAAAAACCTTCTGAAATA157470.4014444556332113No Hit
CCATTTAACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATG145050.36978166183779315No Hit
GCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATA131050.33409091198788554No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA127700.32555062541665764No Hit
TTATAAAAGTGGCTATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTT127520.3250917443471588No Hit
GATTAGGACATTCCCCTGCTCCAAACCCTCCAGGGGCTCCCATTACAGTT113030.2881518182525044No Hit
CTTCTATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGC112310.28631629397450914No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT104600.26666088816431No Hit
ATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTT104360.2660490467383116No Hit
ACTCAATACTCAGTAAGTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTT104210.26566664584706257No Hit
GTGTCAGGACTTGGCAACCTCTTCTGTAAAGGACCGGATAGTAAATACTT99310.25317488339959493No Hit
GTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTA97010.24731140306711008No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA95580.2436658479038695No Hit
GTCCTGACACAGGGCACTGAGTTTTATTCTAACTGTAATGGGAGCCCCTG95030.2422637113026231No Hit
GTTATAAGGTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCAC92320.23535500186739103No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT90340.23030731010290406No Hit
GTCCAAGTCTGGCCCACCACTTGTTCTTGTAAACCAAGTTTTATTGGAAC85910.21901373711468328No Hit
GTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTT82090.20927526108420852No Hit
CTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTGA81730.20835749894521088No Hit
ATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGCATACT78920.20119385558247943No Hit
GTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTTGACGAGTTTGTTCCCT75870.19341837079374952No Hit
CCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAACAGCAGAACCAAA75800.1932399170445No Hit
CTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATATGC71060.18115604888103126No Hit
CCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAGA69220.1764652646150434No Hit
CCTTATAACTTTCCTCAATTTAGGGAGTGGAGTTGATCCTCATCTTTGCC69030.17598089015279467No Hit
CATTTGGGTGGTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCT68430.1744512865877986No Hit
GTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGGCGTCAAGTGCATCCCA67390.17179997374180545No Hit
CCCCAGTGCCTGGGAAGTGGTTGCACGTGCTCTCCATGTTTGAGGAGTGA67000.17080573142455804No Hit
CGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGAT66230.16884274018281312No Hit
ACTCGGGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACT65270.16639537447881947No Hit
CTCCATGTTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAA63940.16300475324307823No Hit
TCCCTGAACTTACTGAGTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGG63680.16234192503157993No Hit
GCTCCAAACCCTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGC63150.16099077521583344No Hit
CCCTAAATTGAGGAAAGTTATAAGGTGGAGAAGTGGGGAACGCAGTGAAC62970.16053189414633462No Hit
GGGCAGAAGTGTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGAT61750.15742170023084268No Hit
GTTCTAAATTGGTAACAAGGCCGATTTCCACTAGGCATCAACTAGTTAAA59080.1506149643666103No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC57040.14541431224562373No Hit
TTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAATTTCTCA56500.1440376690371273No Hit
GGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAG51960.13246366872865725No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG51630.1316223867679094No Hit
TTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGATGC51560.1314439330186599No Hit
TCCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAG51540.13139294623316003No Hit
CTATAAAAGGCGAGACAATAAACTTTATCTAGAGACAAAACTAACAATAC51340.13088307837816135No Hit
GTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAGATA50940.129863342668164No Hit
AGATAGAAGTGGCTCTCAGTTCTAAATTGGTAACAAGGCCGATTTCCACT50280.12818077874666833No Hit
ACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGG50150.1278493646409192No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC50030.12754344392791997No Hit
GTCCTAATCTAATTTATATTTTTAAAATACCCCCCTGGTGGAGGAAAGAC49930.12728851000042063No Hit
GACCTGAGGATGACTCGGACGTCACCTCCCTCCTCTGAGCCGCAGTGGCC49510.1262177875049234No Hit
GAACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGA48840.12450973019067783No Hit
CTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGCCCTGTGTCAG48480.1235919680516802No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC48450.1235154878734304No Hit
CTTCTGCCCACTTCTCACCACCTCTCCCGCAGCACCGCATCTCCCTGCAG47980.12231729841418351No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA47730.12167996359543515No Hit
CCTCAAGGATGTGGGATGCACTTGACGCCTCTCCCACTGGGCTCTGCCCC47680.12155249663168548No Hit
CCATATGGCCCACTAAGTTGAAAGTATTTACTATCCGGTCCTTTACAGAA47660.12150150984618562No Hit
ACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTG47580.12129756270418614No Hit
CTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGGGTCTGATG46430.11836582253794373No Hit
GTATTTACTATCCGGTCCTTTACAGAAGAGGTTGCCAAGTCCTGACACAG46100.11752454057719591No Hit
GGCTCACACAGAAACCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAG45970.11719312647144675No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG45970.11719312647144675No Hit
GTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGATGACTCGGACG45860.1169126991511975No Hit
GTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCACCTCCGCCT45350.11561253612095085No Hit
GTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCCACC45110.11500069469495243No Hit
CCTCTGAGCCGCAGTGGCCTCATTTCTAAGGCAAAGATGAGGATCAACTC45110.11500069469495243No Hit
CCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCC44650.11382799862845547No Hit
TTACAGAAGAGGTTGCCAAGTCCTGACACAGGGCACTGAGTTTTATTCTA44640.11380250523570554No Hit
GCCATGCCCACTCCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAAC44560.11359855809370606No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC44480.11339461095170658No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT43700.11140612631721172No Hit
CTTCTGTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATG43610.11117668578246233No Hit
GTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTATAG42940.10946862846821674No Hit
GTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATGGTCTC41420.10559363277022678No Hit
GAATAAAACTCAGTGCCCTGTGTCAGGACTTGGCAACCTCTTCTGTAAAG40990.10449741688197962No Hit
CTCCCGAGTATTTCAGAAGGTTTTTGCATATCTTGTAGTTAAATGGTTAC40970.10444643009647975No Hit
GTGTTCCGAGGGTCTGATGTCAGCCATGGAGGATACAGCTGCTGCACCTT40620.10355416135023206No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT40380.10294231992423364No Hit
CCCCAAAGCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTA39820.10151468993023732No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)