FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1335062
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGG65590.49128804504959317No Hit
ATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTTGAA54640.4092693822459182No Hit
CCTCTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTC47510.3558636228130229No Hit
GATCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTG46380.3473995964232373No Hit
GTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCA44520.33346765917987325No Hit
GTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTT40390.30253276626853287No Hit
GTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAA36230.27137316469197686No Hit
CTGTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACA33300.2494266183892583No Hit
GTCTTAACATGCGTGCTGTGAGTCCTCCGCAAAGTAGGGACAGTAATACG32330.2421610382139556No Hit
GGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTTGT30800.23070089628796264No Hit
GTCCGAAGACCTACCAGAGAACATGCAGTTCAACAGGAATGAAAAGAAAA30640.22950245007347975No Hit
TGTTAAGACACTTTCTTTTGGTCATAGCTGGAGCCCATATTTGAAGCGGA30590.2291279356314538No Hit
GTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAG28740.215270901276495No Hit
ATCCAAGCTATCCCCTCAGTGAAACAAATGCTTGAGAAAGAAGATTTTTA27720.2076308066591664No Hit
GTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAGAG27380.2050841084533902No Hit
TGTCTGGACTGAGGAAAATCCCTCTGGTACCCACTGTCCTTTCCTCTATG26480.19834284849692374No Hit
GTCCAGACACCTGAGTCCAGTCCCTGGGGACTTCAGGCTGGGTTATTTTG26370.19751891672446673No Hit
GAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACAGACAC26240.19654517917519934No Hit
TCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTCCAAGATTAGACTAAGGA26180.19609576184476826No Hit
GCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAGCTATTCGTATTA25760.1929498405317506No Hit
TCGTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGA24620.18441091125355977No Hit
GAGTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTG24610.18433600836515457No Hit
CCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAGCTATGACCAAAA24500.18351207659269758No Hit
GCCTTTTATGGGAGGGATTTTTTTTAAAAACAAACTCCACCAGCTCAGGT24440.18306265926226645No Hit
GAATATGGGAGGCGAAGGTTGCAGTGAGCCAAGATCGCACCACTGTACTA23700.17751984552028297No Hit
ACTTAGAGCACTGCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTT23620.1769206224130415No Hit
GCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAG22900.17152761444786832No Hit
GGATAGTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAA22480.16838169313485066No Hit
GAGTATATCCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTA22450.16815698446963512No Hit
GGTTTAGGGGCCGTACTCAGCTGGTTGAGTGTCATAGAGGAAAGGACAGT22090.16546048048704853No Hit
CCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTC21740.16283887939286715No Hit
GTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGC21340.15984276385665985No Hit
GTCTCAACCTGAGCTGGTGGAGTTTGTTTTTAAAAAAAATCCCTCCCATA19660.14725907860458914No Hit
GGTACCCACTGTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCC19380.14516179772924404No Hit
ATAAAAGGCTGGGCAGAGGGGCGTTACACCTGAGGGAATTTTCCAGATTA18950.14194097352782117No Hit
GCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCCTCCGTGGGTATA18890.1414915561973901No Hit
GCTGAGTACGGCCCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAG18350.13744680022351022No Hit
AGCTTGGATAGCCTCATTTTAGTTGATGTCACCATTTCAATCCCTACTTT18270.13684757711626877No Hit
AAATAACCCAGCCTGAAGTCCCCAGGGACTGGACTCAGGTGTCTGGACTG18240.1366228684510532No Hit
AGATTAGACTAAGGACAAAATAACCCAGCCTGAAGTCCCCAGGGACTGGA18110.13564913090178585No Hit
GTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACA18090.13549932512497545No Hit
TCTCAGGGCTCATCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCT18080.13542442223657028No Hit
GCTCTGTGATGAGCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCC17990.13475029624092366No Hit
CCCTAGCTCCCTCATGCTCCACGACTTGGGGACAAGCTAGTTGTCTTCTC17770.13310243269600963No Hit
CAGTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAG17770.13310243269600963No Hit
GGAAAGGACAGTGGGTACCAGAGGGATTTTCCTCAGTCCAGACACCTGAG17620.13197888936993188No Hit
ACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAACA17230.12905767672212975No Hit
ATTCAGGAGACTGCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTAT16900.12658588140475874No Hit
GCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACA16670.12486311497143952No Hit
TCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCTTGTGGTCAGGGA16550.12396428031057732No Hit
CCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTACCAGAGAA15890.11902068967583528No Hit
GGTTGAGACAGGCAAATTTCTTTCTTATCTTTCTATGCCTAAAAATCTTC15830.1185712723454042No Hit
GTTCAACAGGAATGAAAAGAAAAACAAATGTAAAAGTCGTCTCCTCTGTA15820.11849636945699901No Hit
CTACAGATCCTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGA15720.11774734057294718No Hit
AGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGC15560.11654889435846426No Hit
GTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCC15440.11565005969760207No Hit
GAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGC15400.11535044814398132No Hit
GTCTTACTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC15030.11257904127298957No Hit
CTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGGTGCCAGGCAG14990.11227942971936884No Hit
GTTCGTTGAGATGGCATTTCCATGGAATTCAGGAGACTGCATGGAACATA14830.11108098350488592No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT14820.11100608061648073No Hit
TCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTT14720.1102570517324289No Hit
CCCCAGGGACTGGACTCAGGTGTCTGGACTGAGGAAAATCCCTCTGGTAC14540.10890879974113563No Hit
CTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTA14530.10883389685273043No Hit
GTCTTCGGACCATGGGGTTTCTTTGTGTCTGTCCTCAGGATATACTCCCA14500.10860918818751489No Hit
CTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAA14330.10733583908462678No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA14190.10628719864695423No Hit
CCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAGATCTATAC14080.10546326687449721No Hit
CCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGCTCATCCG13930.10433972354841949No Hit
CTTCAAATATGGGCTCCAGCTATGACCAAAAGAAAGTGTCTTAACATGCG13800.1033659859991521No Hit
GTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACACTTTCTTT13720.10276676289191064No Hit
AGCCTGAAGTCCCCAGGGACTGGACTCAGGTGTCTGGACTGAGGAAAATC13650.10224244267307436No Hit
GTACTCAGCTGGTTGAGTGTCATAGAGGAAAGGACAGTGGGTACCAGAGG13640.10216753978466916No Hit
CCTATAATCCCAGCACTTTGGGAGGCCAAGGCGGGTGGATCACGAGGTCA13580.10171812245423809No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA13560.10156831667742772No Hit
ATCCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTACCAGAG13460.10081928779337589No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)