FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1210718
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA63410.523738806229031No Hit
GGATACACCATGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATA43510.35937352876557543No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT36820.3041170611158007No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG36520.3016391926113265No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG32030.2645537606610292No Hit
GTTCATTGATGCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTG31150.25728534638123823No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA30610.2528251830731847No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGTAT30570.25249480060592144No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG30440.251421057587316No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA30370.2508428882696053No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA28570.23597567724276008No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT28320.23391078682236494No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG27580.22779871117799522No Hit
ATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGCCT26950.2225951873185994No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG24850.20525010778727992No Hit
CATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATC24770.20458934285275351No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC24520.2025244524323583No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC23730.1959993987039096No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA22010.1817929526115908No Hit
GCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCAT21030.17369858216364176No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG20970.1732030084627469No Hit
GTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTG20840.17212926544414142No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG20040.16552161609887686No Hit
CTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGC19950.1647782555475346No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG19460.16073107032356007No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG19180.15841839305271746No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT18970.15668388509958553No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG18850.15569273769779585No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGCTAATGTGCTAATTGGGCAA18260.15081959630566324No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG18000.14867211026845226No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG17450.1441293513435829No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC16970.14016476173642417No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC16870.1393388055682661No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA16490.13620017212926544No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG16380.13529162034429157No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA16340.13496123787702835No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG16310.13471345102658092No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT16300.1346308554097651No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT15850.1309140526530538No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCATGTCCATTATTCTCT15820.13066626580260637No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA15750.13008809648489575No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC15200.12554533756002637No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC15140.12504976385913152No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT15110.1248019770086841No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG15020.12405861645734184No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT14850.12265449097147313No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCGAGA14660.1210851742519728No Hit
TAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACT14500.11976364438291988No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT14470.11951585753247247No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG14290.11802913642978793No Hit
GTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTT14280.11794654081297214No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA14210.1173683714952615No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCG14140.11679020217755084No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT13910.11489050299078728No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGT13670.11290820818720793No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT13620.1124952301031289No Hit
TCTATACACAGGTTGGATAGAAGAGCCTTTGTGGTCTAGAAAGGTTAGAT13590.11224744325268148No Hit
CATATAGACTGTTGAATACAGATTAAACTTCCAAATGTGTGCATATTGCA13190.1089436185800492No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA13020.10753949309418047No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT12990.10729170624373305No Hit
AATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTA12960.10704391939328564No Hit
ATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACGCA12890.10646575007557499No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA12860.10621796322512758No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA12750.1053094114401537No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA12750.1053094114401537No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA12650.10448345527199562No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCTCCTTTTGCAAGATTGC12420.10258375608523207No Hit
CTTATATGCAAACAACGACAACGTGTATCTCTGCGTTGAATACTCTTGCG12410.10250116046841627No Hit
ATCTCAGAGAACTCTTCTTTCTTGATCCGTCCAGACTCGAAGTCGACCCT12390.10233596923478465No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGCTAATGTGCT12380.10225337361796884No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT12230.10101443936573173No Hit
GCCATGGAACAGGAACAGGATACACCATGCAATCTATTCGAGAAATTTTT12130.10018848319757367No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA12120.10010588758075786No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)