FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4824233
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA167210.3466043203137162No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT135720.28132969531115104No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT133760.277266873304005No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT117090.24271215755955403No Hit
ATTAGCACATTAGCCTTCTCTCCTTTTGCAAGATTGCTCAGTTCATTGAT116350.24117823496501933No Hit
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA102650.21277993828241715No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC101980.21139111647385192No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA100990.20933897678656896No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC81960.16989229168657483No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG79620.16504177969845155No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT77890.1614557174166339No Hit
GAGCAAAAGCAGGGGAAAACAAAAGCAACAAAAATGAAGGCAATACTAGT75710.15693686436786947No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT72700.15069753057118096No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA72350.14997202664133344No Hit
ACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACC71510.14823081720969944No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG68710.14242678577091944No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG67650.140229545297667No Hit
GCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGA67270.1394418553166897No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC66290.13741044431311672No Hit
GAGCAAAAGCAGGTAGATATTTAAAGATGAGTCTTCTAACCGAGGTCGAA65350.13546194804438344No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG65070.1348815449005054No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT63910.1324770175901537No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC63720.13208317259966507No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA62760.13009321896351192No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT62760.13009321896351192No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG61300.12706683114186235No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA61270.12700464509073256No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT60760.1259474822215262No Hit
ATCTTTAACCGATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGA59950.12426845884102199No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC58430.12111769891711283No Hit
GTGTTACCATTCCATTCAAGTCCTCCGATGAGGACCCCAACTGCATTTTT57640.11948013290402847No Hit
ATTAGAGACCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAG56910.1179669389932037No Hit
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG56550.11722070637964627No Hit
CTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACC56470.11705487690996683No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC56260.11661957455205833No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA55230.11448452012993568No Hit
ACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAAATTTCACCAT54650.11328225647475983No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT53860.11164469046167547No Hit
GAAAAGAACATAGTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATT53230.11033878338794997No Hit
CCTTGATACTACTAAGGGCTTTCACTGAGGAGGGAGCAATAGTTGGAGAA53120.11011076786714075No Hit
GTACTGAAAGCGAACTTCAGTGTAATCTTTAACCGATTAGAGACCTTGAT52760.1093645352535833No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA52630.10909506236535425No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC52600.10903287631422445No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC52100.10799644212872803No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA51500.1067527211061323No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA50880.10546754271611675No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT50360.10438965116320044No Hit
GTTGTATATGAGGCCCATGCAACTGGCAAGTGCACCAGTTGAATAGCTTA49810.10324957355915439No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT49270.10213022463881824No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA48810.1011767051881615No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA48300.10011954231895516No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)