FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_F10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2376747
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTC620502.6107111947548476No Hit
TTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGT290841.2236893535576145No Hit
GGGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTG230350.9691818270939229No Hit
GGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGA222340.9354803014372165No Hit
CCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGA221120.930347235107481No Hit
GTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAG179040.7532985210457823No Hit
GATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAG145390.6117184538362729No Hit
GGTAGAAAGTGAGTGATTCATCACCAAGGTCATGCTGTCTGTCATGTTGT144160.6065433131923591No Hit
GTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCACCCACATTGTGGA126010.5301784329589981No Hit
TGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCA123030.5176402873339063No Hit
GTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGA117630.4949201576776998No Hit
ACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGG116870.49172250980015964No Hit
GTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACT112370.47278906841998747No Hit
AAATAAAGAAGACCCAGAGTCCACAATGTGGGTGGACATTATCCAATTCC112150.471863433508068No Hit
CTTCAGGAAAACTCTCAGAGAAACATAAATCATGTTTCATCAACTATTTG110900.46660414423579794No Hit
GTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCA107810.4536031811547464No Hit
CTTTTATGCTGGGTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTT103350.4348380370312869No Hit
TTCCTGAAGAGATTGGGATTAGAATCAGTAGACTAAATAAAGAAGACCCA103070.43365995623429837No Hit
GTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCACCCACATT101530.4271805118508617No Hit
GGCCCAGGCTCATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCCTTCTG95310.40101028843204595No Hit
ACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAAT94390.3971394515276552No Hit
CAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAA92100.3875044335808565No Hit
TTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCACCC87060.3662989792350637No Hit
GTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGAGTGT87010.36608860766417295No Hit
GTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAA86550.36415318921197753No Hit
GAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTTTACTGTTTTGTTC85130.35817863659867877No Hit
ACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCC80610.33916104659015034No Hit
GGATTAGAATCAGTAGACTAAATAAAGAAGACCCAGAGTCCACAATGTGG79770.3356268041991849No Hit
AGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACA79360.3339017573178803No Hit
CCCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCATTACACTCCATTG79350.3338596830037021No Hit
AAGTTAACCATTACACTCCATTGTTGATTCAGTAAAACCCAGCATAAAAG78960.3322187847507539No Hit
CATTAACACATGTAACATCTCCATTTCAATGATGAACAGACATATCAAAT77740.3270857184210183No Hit
GTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTG76150.3203959024666908No Hit
ATTCAGGCCTTCAAGGAATTGGATAATGTCCACCCACATTGTGGACTCTG75590.3180397408727138No Hit
CTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGAGT75240.3165671398764782No Hit
GTTGCAGAGGGTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGGTGGGA74270.3124859314011967No Hit
ATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGA73010.3071845678147485No Hit
ATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAGTAGAC68820.2895554301740993No Hit
GTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACAT67890.2856425189555304No Hit
GAATTGGATAATGTCCACCCACATTGTGGACTCTGGGTCTTCTTTATTTA67850.28547422169881775No Hit
CCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGATAATGTCCA67380.2834967289324442No Hit
ATTCATCACCAAGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGG65130.2740300082423582No Hit
GAGCTGGACATGGTGGGTGGGAAGAGGTGGGTGGCAGAAGGCAGCTTGGG64450.2711689548782432No Hit
GGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCAAATAGTT63990.26923353642604786No Hit
ATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTT62960.26489988206569737No Hit
ATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATG58310.24533532597285282No Hit
GACAAAGTGTGTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGG58260.24512495440196203No Hit
GTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATCA58180.24478835988853673No Hit
GGCAAAGGCCACTGGTGGGGGTTTGCAGGGATCAGTTGCAGAGGGTCTAG58090.2444096910609333No Hit
GAGACAAAGTGTGTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAA57110.2402864082714736No Hit
CATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCCTTCTGCCACCCACCT56750.2387717329610598No Hit
CATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCAA56310.23692046313722073No Hit
AAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAG55730.23448015291488744No Hit
ATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAGT55470.23338622074625526No Hit
GGGTTTTACTGAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAA54760.23039894443960587No Hit
CTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAA54110.22766411401802547No Hit
GGATAATGTCCACCCACATTGTGGACTCTGGGTCTTCTTTATTTAGTCTA52970.22286764220171518No Hit
ACATAAATCATGTTTCATCAACTATTTGGGCATCCCTTAACCCAGTCAAG52040.2189547309831463No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT51710.217566278615267No Hit
ATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGTTTCAT51700.21752420430108882No Hit
GTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCAAA51400.21626197487574403No Hit
CTATTTGGGCATCCCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCAT51390.21621990056156587No Hit
GTTTTGAGGGATGATCAAAATTTGTTTTTAGACATGCTAGATTTGATATG50230.21133928011689926No Hit
GCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTG49320.20751051752668667No Hit
TAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGAT49280.20734222026997404No Hit
TTACATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAATG49000.20616413947298554No Hit
ATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCAAT47730.20082070157235918No Hit
ACATTGTGGACTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCA46700.19648704721200871No Hit
ACATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAATGAT46600.19606630407022707No Hit
TCACCAAGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTA46530.19577178387097993No Hit
TCCCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCATTACACTCCATT46150.19417295993220984No Hit
CTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGAATCAGTAGACTAAATA45690.1922375414800145No Hit
GTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGGTGGGAAGAGGTGGGT45400.19101738636884785No Hit
AGCGAAAGCAGGGGCCCAGGCTCATCAGCAGCTGGCCAAAGCCCTTCCCA45150.18996552851439383No Hit
AGGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAAT44680.1879880357480203No Hit
GAGATGAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTTTACTGTTT44680.1879880357480203No Hit
CATTACATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAA44400.1868099549510318No Hit
AACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGATTAGA44260.18622091455253756No Hit
GGCCACTGGTGGGGGTTTGCAGGGATCAGTTGCAGAGGGTCTAGTTGGGA42660.1794890242840319No Hit
CTCTGGGTCTTCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAG42580.17915242977060664No Hit
TTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGA40930.1722101679312102No Hit
GATCAGTTGCAGAGGGTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGG39870.16775029062832517No Hit
CTGGTGGGGGTTTGCAGGGATCAGTTGCAGAGGGTCTAGTTGGGAGGGAA39160.1647630143216758No Hit
CCATTACACTCCATTGTTGATTCAGTAAAACCCAGCATAAAAGTCACAAC38930.16379530509557813No Hit
GGACATGGTGGGTGGGAAGAGGTGGGTGGCAGAAGGCAGCTTGGGAAGGG38200.1607238801605724No Hit
TGCCCAAATAGTTGATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCT38140.16047143427550345No Hit
CATGTAACATCTCCATTTCAATGATGAACAGACATATCAAATCTAGCATG37460.15761038091138854No Hit
CTCTCAGAGAAACATAAATCATGTTTCATCAACTATTTGGGCATCCCTTA36990.15563288814501502No Hit
CTCTTCAGGAAAACTCTCAGAGAAACATAAATCATGTTTCATCAACTATT36750.15462310460473916No Hit
GGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGC35440.14911136944740017No Hit
GTCCACAATGTGGGTGGACATTATCCAATTCCTTGAAGGCCTGAATAGAA35410.1489851465048657No Hit
GATGAAACATGATTTATGTTTCTCTGAGAGTTTTCCTGAAGAGATTGGGA34260.14414660037437724No Hit
AGCAGGAGGAGATGAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTT33970.1429264452632106No Hit
GATGAATATCCCAGCTCGGAGACAAAGTGTGTCCTACCTTTACTGTTTTG33590.1413276213244405No Hit
CTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTCTCA33480.14086480386848074No Hit
TGCTAGATTTGATATGTCTGTTCATCATTGAAATGGAGATGTTACATGTG33360.1403599120983428No Hit
GAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGG33290.1400653918990957No Hit
GGTCATGCTGTCTGTCATGTTGTGACTTTTATGCTGGGTTTTACTGAATC32730.13770923030511872No Hit
ACATTATCCAATTCCTTGAAGGCCTGAATAGAACAAAACAGTAAAGGTAG32290.13585796048127965No Hit
ATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGAT32140.13522684576860727No Hit
GAAAGTGAGTGATTCATCACCAAGGTCATGCTGTCTGTCATGTTGTGACT32130.1351847714544291No Hit
CATAAATCATGTTTCATCAACTATTTGGGCATCCCTTAACCCAGTCAAGT32020.13472195399846934No Hit
GATTGGGATTAGAATCAGTAGACTAAATAAAGAAGACCCAGAGTCCACAA31590.13291275848880843No Hit
CCTTAACCCAGTCAAGTTGGCACACAAAGTTAACCATTACACTCCATTGT31470.13240786671867052No Hit
ATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCCCAAATAG30770.1294626647261993No Hit
AATCTAGCATGTCTAAAAACAAATTTTGATCATCCCTCAAAACCTGTCAT30690.129126070212774No Hit
CTCATCAGCAGCTGGCCAAAGCCCTTCCCAAGCTGCCTTCTGCCACCCAC29720.12504486173749246No Hit
AGCGAAAGCAGGCAAAGGCCACTGGTGGGGGTTTGCAGGGATCAGTTGCA29310.1233198148561879No Hit
GATCAAGAAAGAAGAGTTCTCTGAGATCATGAAGATCTGTTCCACCATTG29170.12273077445769365No Hit
GTATAAGGGAATGTGGTGCTTATGGCATTTTGCGCTGGAATTTTTAGGAA29100.12243625425844654No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT28670.12062705874878563No Hit
GACATATCAAATCTAGCATGTCTAAAAACAAATTTTGATCATCCCTCAAA28550.12012216697864771No Hit
GGGTCTAGTTGGGAGGGAAGGAGCTGGACATGGTGGGTGGGAAGAGGTGG28510.11995386972193506No Hit
CTTCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAACTC28480.11982764677940058No Hit
GTTGTGACTTTTATGCTGGGTTTTACTGAATCAACAATGGAGTGTAATGG28270.11894408618165922No Hit
GGTTTTGAGGGATGATCAAAATTTGTTTTTAGACATGCTAGATTTGATAT27570.11599888418918801No Hit
AGAATCAGTAGACTAAATAAAGAAGACCCAGAGTCCACAATGTGGGTGGA27560.11595680987500984No Hit
ATATGTATGTGTACACATTAACACATGTAACATCTCCATTTCAATGATGA27350.11507324927726846No Hit
GAGTGTAATGGTTAACTTTGTGTGCCAACTTGACTGGGTTAAGGGATGCC27300.11486287770637767No Hit
AAACAATGGGAAGATATGGCTGCCATTTAATTAAATGACAGGTTTTGAGG27230.11456835750713054No Hit
GGTTTGCAGGGATCAGTTGCAGAGGGTCTAGTTGGGAGGGAAGGAGCTGG26790.11271708768329149No Hit
TATCAAATCTAGCATGTCTAAAAACAAATTTTGATCATCCCTCAAAACCT26490.11145485825794668No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCAGCACTTCT25700.10813098743787201No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA25670.10800476449533754No Hit
GAGAAACATAAATCATGTTTCATCAACTATTTGGGCATCCCTTAACCCAG25590.10766816998191225No Hit
TTCCTGAAGAGATTGGGATTAGAATCAGTAGACTAAATAAAGACTGTCTC25570.10758402135355592No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA25480.10720535252595248No Hit
AACTTGACTGGGTTAAGGGATGCCCAAATAGTTGATGAAACATGATTTAT25470.10716327821177432No Hit
GTCCAGCTCCTTCCCTCCCAACTAGACCCTCTGCAACTGATCCCTGCAAA25460.10712120389759618No Hit
GAATAGAACAAAACAGTAAAGGTAGGACACACTTTGTCTCCGAGCTGGGA25040.10535408270211342No Hit
GAGCAAAAGCAGGCAAACCATTTGAATGGATGTCAATCCGACTCTACTTT25040.10535408270211342No Hit
GTGTCCTACCTTTACTGTTTTGTTCTATTCAGGCCTTCAAGGAATTGGAT24670.10379733307752151No Hit
GTCTTCTTTATTTAGTCTACTGATTCTAATCCCAATCTCTTCAGGAAAAC24560.10333451562156175No Hit
GATTCTAATCCCAATCTCTTCAGGAAAACTCTCAGAGAAACATAAATCAT24280.10215643482457326No Hit
AACCATTACACTCCATTGTTGATTCAGTAAAACCCAGCATAAAAGTCACA24120.1014832457977227No Hit
TTACTGAATCAACAATGGAGTGTAATGGTTAACTTTGTGTGCCAACTTGA23840.1003051650007342No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)