FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3520944
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCAT674851.9166734830204628No Hit
ATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGC531001.5081182773710686No Hit
GTATAAAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTT463281.3157834944264948No Hit
GTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTA414171.1763038548752835No Hit
AGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACAT398371.1314295257181028No Hit
ATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAA303210.8611610977056153No Hit
GTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCT296480.8420469056025883No Hit
GTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGCTG296380.8417628908610872No Hit
CTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTT282290.8017452137835761No Hit
ATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAA271050.7698219568388478No Hit
CACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCT255460.7255440586388195No Hit
CACACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGG252010.7157455500570301No Hit
TCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTC205420.5834230819916477No Hit
CACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCC185240.5261089071567171No Hit
GTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTG179440.5096360521496508No Hit
ATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGATCTGCAGATTCT167770.4764915318164674No Hit
GAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACA167250.4750146551606615No Hit
GTATCAGACTTCCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCC162390.4612115387237059No Hit
GCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCA156750.4451931073030414No Hit
ACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGG156300.4439150409662863No Hit
CCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAAT154640.4392003962573674No Hit
ACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGC152560.4332928896341436No Hit
CTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTC147490.41889334224003566No Hit
TGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATG142180.403812159466325No Hit
GAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAG138750.39407045383283573No Hit
CCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTG130900.37177529662499603No Hit
ATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTAT130270.3699860037535388No Hit
GTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAA129410.367543476976629No Hit
CTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGT123030.3494233364688561No Hit
ACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAA121360.34468029028578695No Hit
AGCGAAAGCAGGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCC114680.32570810555351065No Hit
CCTTTTACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCC113770.3231235714058503No Hit
GTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGACT111490.31664803529962415No Hit
ACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTA109930.3122174053322063No Hit
GGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGC109000.3095760682362457No Hit
ATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCA104570.2969942151877451No Hit
CTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATA102720.291739942469974No Hit
ACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCA101520.2883317655719603No Hit
GGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATT101050.2869968962869049No Hit
GGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGT98390.2794421041629745No Hit
CTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAA98310.27921489236977354No Hit
TCCGTATCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAG96550.2742162329193535No Hit
AGCGAAAGCAGGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGA96400.27379021080710175No Hit
ATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGT95870.2722849326771457No Hit
CTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCT93670.2660366083641205No Hit
GGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGA92180.2618047887157535No Hit
GTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGA91750.26058352532729856No Hit
GTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGGCAGTGGCTCAG91370.25950426930959425No Hit
TTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTA90230.2562665012564812No Hit
GAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTT88000.24993297252100571No Hit
TGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAA87840.24947854893460392No Hit
AAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTAC85280.24220777155217463No Hit
ATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTAT85140.24181015091407307No Hit
GAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATA83010.2357606369200987No Hit
GAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCGTGCTCGACCCGCCC82430.23411335141939205No Hit
ATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTATAC82260.23363052635884013No Hit
GCCCAAGGCTTCGGCCTGATTGTTCGATTCGAAATGCTGAACGCCGGGAC81690.2320116423322836No Hit
ACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAG79600.22607573423490973No Hit
GCCCAATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAA79590.2260473327607596No Hit
GTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGAAGGCAGATGC79240.2250532811655056No Hit
AAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCGGCCC77110.21900376717153128No Hit
GTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATA75150.21343707823810887No Hit
ACATAAACTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATT74390.21127856620270022No Hit
GATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAAC74380.21125016472855004No Hit
GGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGG73520.20880763795164026No Hit
TTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTA71620.20341135786311854No Hit
TTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAA69640.1977878659813959No Hit
GGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAGCAGGGGAAGTC69430.1971914350242435No Hit
GTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAA68090.1933856374881282No Hit
AGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAA66810.18975024879691355No Hit
GATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCAT64810.1840699539668907No Hit
TCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAG63950.18162742718998087No Hit
GTTAAGCATCTGCCTTCAGCTCAGGTCTTGATCCTGGAGTCCTGGGTTCA62680.17802043997291633No Hit
ATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCATGAAATCGAGT62400.17722519869671316No Hit
GCCTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCT59380.16864795350337863No Hit
GCTCAGTGAGGTTGCAAGCACGCTGCAGCTGACGATGGCGAAGGTTCTGG59340.16853434760677818No Hit
CACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCC59060.16773910633057498No Hit
GTATATTTGTTCGTAGCGCGGAGTGCGTGTAAAAGGAGCGCTTCAGCGTG59020.16762550043397453No Hit
GGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAG58850.1671426753734226No Hit
GAAAGAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGC57160.16234282624205326No Hit
CACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTA56910.16163278938830042No Hit
GTTCTGGCTCGAGGCAGCACGCTGAAGCGCTCCTTTTACACGCACTCCGC56500.16046832894814572No Hit
GTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTT56440.16029792010324506No Hit
GAGCTGAAGGCAGTGGCTCAGTCAGTTAAGCATCTGCCTTCAGCTCAGGT56330.1599855038875938No Hit
AGCAGGAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACA56160.15950267882704183No Hit
AGCGAAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCG56130.1594174744045915No Hit
GTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCC55540.15774178742973474No Hit
ACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCA54910.15595249455827756No Hit
AGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCG53590.15220349997046245No Hit
AATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCC53530.1520330911255618No Hit
GAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATA53060.1506982218405064No Hit
CTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTGGTTTGAGTTATGA52640.14950535992620162No Hit
CTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGC52550.14924974665885057No Hit
CAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATA52040.14780127147719474No Hit
TTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTT51530.14635279629553893No Hit
ATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAGAGGG51520.14632439482138881No Hit
AACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTA51460.14615398597648813No Hit
GAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGA51370.14589837270913708No Hit
CTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAGGACTCGGA50440.14325703561317646No Hit
GTTCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGG50270.14277421055262451No Hit
CAAATATACCGGCGGGCTGCTCCGAGCGCGGCCCGCCGGTCCCGGCGTTC50060.14217777959547212No Hit
CTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCA49790.14141093979341904No Hit
CTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCTCT49370.14021807787911422No Hit
GCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTT49090.13942283660291105No Hit
GAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGT48450.13760514225730372No Hit
GGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTC47970.13624187149809824No Hit
GATTATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAG47420.13467979041984196No Hit
TATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAG47330.13442417715249091No Hit
GAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAAC47150.13391295061778885No Hit
GTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGAAA47130.13385614766948864No Hit
GTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGA46900.133202913764036No Hit
GAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGT46460.13195324890143098No Hit
TTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAA46370.13169763563407996No Hit
CTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGA46150.13107280320277745No Hit
GTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAG45850.130220758978274No Hit
CCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTT45560.1293971162279207No Hit
CTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATA44960.12769302777891384No Hit
GTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTAT44650.12681258208026028No Hit
CTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTA43430.12334760223394635No Hit
TACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATA42790.12152990788833905No Hit
ACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGT42490.12067786366383561No Hit
ACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACG41890.11897377521482874No Hit
ACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAA41710.11846254868012669No Hit
ATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCA41690.11840574573182647No Hit
GAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCT40920.11621883222226767No Hit
GGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAATC40780.11582121158416607No Hit
GAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAG40720.11565080273926538No Hit
GGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTAC40210.11420232755760953No Hit
GTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGC40150.11403191871270886No Hit
CTCCAGGATCAAGACCTGAGCTGAAGGCAGATGCTTAACTGACTGAGCCA40140.11400351723855875No Hit
CTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTG39940.11343548775555647No Hit
CTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGA39350.11175980078069972No Hit
AAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGT39070.11096455950449652No Hit
AGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAGCAGGGGA39050.1109077565561963No Hit
TCCTAGAACAGAGCCCCGCATTGGGCTTCCTGCTCAGCAGGGAGTCTGCT38590.10960128874529104No Hit
GCTGAAGGCAGATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCA38570.10954448579699082No Hit
CTCTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAA38120.10826641946023567No Hit
GCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTTGTGCC37750.10721556491668142No Hit
GAGCAGGGGAAGTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGG37530.10659073248537891No Hit
CCAATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTAT37260.10582389268332583No Hit
GCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCGGCCCGCG37040.10519906025202332No Hit
TACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCCGAGCG36800.10451742487242058No Hit
CCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTATT35930.10204649662136063No Hit
GACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATT35830.10176248187985948No Hit
ATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCT35670.10130805829345767No Hit
GGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAAC35600.10110924797440686No Hit
ACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGA35480.10076843028460548No Hit
GAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCC35260.10014359785330297No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)