FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1362568
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC62480.4585459221117772No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC61990.45494977131416564No Hit
CTCATATACTGCACCCATTTCAGCAGCAAATCCTGTTGCCCCTACTTTCA39290.28835258130236435No Hit
GTATATGAGAAGAAAAAAGAAATGCAGAATAACACAAAGATTCTAGACAA37470.27499544976837853No Hit
GTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCA33100.2429236559202917No Hit
CCCTCATACTGCAGCTTCTGTTTCCCTGCAGGCATACCCGTGGCTTCGTG28200.206962147944176No Hit
GTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGACCTGACAGCAA27660.20299904298354282No Hit
AAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATA26090.19147668226466497No Hit
GTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAA24960.18318351818037706No Hit
GTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGT24820.1821560465239166No Hit
GTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAATGGAAAC24750.1816423106956864No Hit
CTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTAATAAG24050.17650495241338415No Hit
GACCAGGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCC23370.17151437579629053No Hit
GTATGAGGGCATTTTCATCAAGGATTCCAACTCATTGGCTTATTACAACA22100.16219374005554218No Hit
ACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACACACGGT21020.15426753013427588No Hit
GTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACT20220.14839626352593044No Hit
GGCTGAGACCTGACAGCAAGTTTCTCTTCTCTACTTCTTCCGCCCGCCTC19600.14384603190446274No Hit
CCATAAAATTCGCCCTTTTAGAACTGTACAACTCAGTGATTTTAGTCTTT17770.1304155095378726No Hit
GTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAG17760.1303421187052683No Hit
GGCATACCCGTGGCTTCGTGAATCTTCACCTTGATGACAGAGACCTGGTC17280.12681935874026104No Hit
ATTCTAGACAATGAGCTTTTTCTTTCCAGACACAGGTGCTGCTCCTTCTT16910.12410389793390128No Hit
GTCTAGAATCTTTGTGTTATTCTGCATTTCTTTTTTCTTCTCATATACTG16630.1220489546209804No Hit
TGTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATAC16310.11970044797764222No Hit
GACAGGTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATC15720.11537038885398748No Hit
GAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCCCGCTCACAGA15210.11162745639116725No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA15200.11155406555856295No Hit
ATAATAACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACA15120.11096693889772842No Hit
GGGTATGCCTGCAGGGAAACAGAAGCTGCAGTATGAGGGCATTTTCATCA15100.11082015723251977No Hit
CTCTTAATCTTGGCTTAAATAAATAGGGTGCTTATTTTTCTTCTGTAAGA14820.10876521391959887No Hit
GACTATAGGCACGCCAGAGTGGGAGCAGGGAGAGGAAACATTGGCCATTA14670.10766435143053411No Hit
ATAACATACTAAGGATTTCTGAATGGAAAGGCAGAAATAGCCTCCTGGCT14650.10751756976532546No Hit
GGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGA14540.10671027060667798No Hit
GGGTATATGGAATCTATATCAAACAAGAAAGGCAGGAGGAAGATAGAAGC14540.10671027060667798No Hit
GGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCCTGCAG14310.10502228145677867No Hit
CCTCTCTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTA14140.10377463730250527No Hit
GTTCTAAAAGGGCGAATTTTATGGTATGTGAATTATGTCTTAATAAAAAA14060.10318751064167073No Hit
TCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGTGAAGATTCAC14000.10274716564604482No Hit
GTATGTTATTGTCCTCATGCTTGTCTCTGTAGGTTACCAAATACCTGTTC13840.10157291232437574No Hit
GAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCAGCTTCTGTTTCCC13780.10113256732874983No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)