FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1141968
Sequences flagged as poor quality0
Sequence length151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT78230.6850454653720595No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG70040.6133271685371219No Hit
GAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCG68480.5996665405685623No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG62290.5454618693343422No Hit
ATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTC60910.5333774676698471No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC59360.5198044078292912No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA59200.5184033177812338No Hit
AGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCT56590.4955480363722976No Hit
GGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGAC56290.49292099253219No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA54750.4794355008196377No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG54280.47531979880346914No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT52540.460082944530845No Hit
ATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGT51700.4527272217785437No Hit
GTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGC50490.44213147829010974No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG49610.4344254830257941No Hit
ACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCA49120.4301346447536183No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG46750.4093809984167683No Hit
GACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCA44070.385912740111807No Hit
GGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCA42130.3689245232791111No Hit
GTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACC41370.36226934555083856No Hit
CCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCA41260.3613060961427991No Hit
GAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGC37330.3268918218373895No Hit
GCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCA36730.3216377341571743No Hit
GAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCC36720.3215501660291707No Hit
GAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAG36200.3169966233729842No Hit
GTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACT35800.3134938982528407No Hit
CACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGA35720.312793353228812No Hit
CGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTC34140.2989575890042453No Hit
TCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGA33320.2917770025079512No Hit
GGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTT32720.286522914827736No Hit
ATTTATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAG31600.27671528449133426No Hit
CTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAG31080.27216174183514774No Hit
ATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCC30810.2697974023790509No Hit
AAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGT30630.26822117607498636No Hit
GCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTC30370.26594440474689307No Hit
ATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCTCAGCAGG28740.25167079988230845No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA28510.24965673293822593No Hit
TTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAA28310.2479053703781542No Hit
CTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAA25580.22399927143317502No Hit
TAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTAT25450.22286088576912838No Hit
AGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGA25160.22032141005702438No Hit
GGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGT23930.2095505303125832No Hit
GTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATC23410.20499698765639668No Hit
GGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCT23200.20315805696832137No Hit
AGCGAAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGG23130.20254508007229627No Hit
AGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTG23020.2015818306642568No Hit
GCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCTCATTTC22750.19921749120815996No Hit
CATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGA22060.19317529037591244No Hit
AGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAG21880.1915990640718479No Hit
GGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGG21800.1908985190478192No Hit
GCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTT21370.18713308954366498No Hit
GCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATG21320.18669524890364705No Hit
CTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGGACTCCAG20950.18345522816751433No Hit
AAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGC20630.18065304807139956No Hit
GGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGG19880.17408543847113053No Hit
GAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCA19730.17277191655107674No Hit
GAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGT19620.17180866714303727No Hit
GAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGA19560.17128325837501576No Hit
ACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAAAAGCTCACAG19380.1697070320709512No Hit
GCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCT18840.1649783531587575No Hit
CCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCA18260.1598994017345495No Hit
GTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAA17750.15543342720636655No Hit
TGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAA17450.15280638336625896No Hit
GCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTC17350.15193070208622309No Hit
CCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAA17270.1512301570621944No Hit
GGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTG17000.14886581760609754No Hit
TCCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAA16930.14825284071007244No Hit
GAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGAC16830.14737715943003657No Hit
CTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATC16550.14492525184593613No Hit
GGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATC16520.1446625474619254No Hit
GAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCC16510.1445749793339218No Hit
GGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGT16400.14361172992588234No Hit
AAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCT16210.14194793549381418No Hit
GTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCG15680.13730682470962408No Hit
GAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGC15650.13704412032561333No Hit
GAAAGAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCC15580.13643114342958823No Hit
GAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTG15550.13616843904557746No Hit
AGCGAAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCC15360.1345046446135093No Hit
AATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGT15320.13415437210149497No Hit
GAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTG15210.1331911226934555No Hit
GATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCC15030.13161489638939095No Hit
GTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCA14950.13091435136536225No Hit
GCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGT14590.12776189875723312No Hit
GAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAAC14130.12373376486906813No Hit
GAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGC14110.12355862861306095No Hit
CAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTG13800.12084401664494977No Hit
GCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGG13720.12014347162092108No Hit
ACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACCTCTCC13410.1174288596528099No Hit
CTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCA13060.11436397517268435No Hit
GAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGG13030.11410127078867359No Hit
AACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCAC12940.1133131576366413No Hit
AGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGC12870.1127001807406162No Hit
GGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTTTCTTTCTTTC12780.11191206758858392No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA12540.10981043251649784No Hit
CAACTGAGCCACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAA12510.10954772813248709No Hit
GGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTC12390.10849691059644405No Hit
CTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAG12330.10797150182842252No Hit
CTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAAAGAAAGAAAG12270.10744609306040098No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA12120.10613257114034719No Hit
ACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCT12090.10586986675633642No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG11970.10481904922029339No Hit
GAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTT11770.10306768666022165No Hit
ATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGAC11700.10245470976419654No Hit
GCCTGACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAA11630.10184173286817144No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT11580.1014038922281535No Hit
CTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAAAGAAAGAAAGAAAG11450.10026550656410688No Hit
CCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAAAAGCTCACAGC11440.1001779384361033No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)