FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3965826
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTG221730.5591016852479156No Hit
GTCCAGGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAG188420.4751090945492818No Hit
GTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGG162710.41028022913763745No Hit
GGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGCAGCGGGGCTGGGCAG137490.3466869196984437No Hit
GAATTGGATGGGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTA136420.34398886890145963No Hit
GTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGCCCCAGGACTT131090.33054904577255784No Hit
GCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTG127130.320563736280916No Hit
CTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCA108630.2739151944639024No Hit
CAGTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGG108560.273738686467838No Hit
GGGCTGGGCAGCGAGACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAG97910.24688425563804361No Hit
GTATTCAGTAAATGCCATCACCATCTTCCCAGTGGCTGAGGCCAAAACCT95960.24196724717625032No Hit
ACTGAATACAGACTGAACTATGTATTGCTGTGCATGATGGAGCGCACTGG94920.23934484266329384No Hit
GAACTATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCA93300.235259943325804No Hit
GCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCA90270.22761966863901745No Hit
TCCTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTC86770.21879426883579864No Hit
GGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACT85930.21667617288302615No Hit
GGCAAGCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTC85340.21518846263048355No Hit
TTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGG79840.20131997722542544No Hit
ACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCAGTGGC77990.19665512304372404No Hit
CCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGGAGAAAG76370.1925702237062342No Hit
CACTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGT72020.18160151252223372No Hit
ATGCTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTT71810.18107198853404058No Hit
GTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGT70810.17855044573312093No Hit
GAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGGTTTT68590.17295262071507927No Hit
GCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGTGT64300.16213520209913396No Hit
ATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTG62660.15799987190562573No Hit
GGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTGGCCTC61270.15449492741234738No Hit
CAGCGGGGCTGGGCAGCGAGACTTGCACCGGAGCCTTTGCCACAGTCCAG60620.1528559245917496No Hit
GTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGGTTGCATGGCA60020.15134299891119782No Hit
GGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGG59490.1500065812267104No Hit
GAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTG58010.1462746978813493No Hit
GCACTAGTGCGTGGGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGCAG57730.1455686658970918No Hit
GGACTGGGGGGCCGAGGCCGAGAAACACGCAGCGGGGCTGGGCAGCGAGA56760.14312276938019974No Hit
ACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTT56390.14218979854385946No Hit
GAGCACTAGTGCGTGGGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGC56070.1413829048475652No Hit
GAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGC55770.14062644200728927No Hit
GATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGA55060.13883614661863633No Hit
GGGGAAGGAATTGGATGGGCGTAGTAGGTATTTTGGAAGGTGAACAGATG53850.13578507982952354No Hit
GACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAGAAAGGGCCAGTGCC53510.13492775527721085No Hit
TAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAAACCCCCGT53460.13480167813716487No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT51970.1310445793637946No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA51810.13064113251564743No Hit
GGATTTGAGGATGAGCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGT51620.1301620393834727No Hit
GTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAA51550.12998553138740832No Hit
CTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGG51480.12980902339134395No Hit
AGCGAAAGCAGGGGAAGGAATTGGATGGGCGTAGTAGGTATTTTGGAAGG51350.1294812228272244No Hit
ACCTAGGGCTCATCCTCAAATCCTCTGCTGCCATGCAACCCTCTTCAGTA51230.12917863769111404No Hit
ATCATGCACAGCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCAC50080.12627886347005643No Hit
GGTTAAAACCCCCGTGGCCTTTGATTCCTCTCTTCTTTCTCCACTCCCTT48860.12320258125293444No Hit
GCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTT48670.12272348812075971No Hit
GGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCT48500.12229482584460337No Hit
GTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTGAATACAG48430.122118317848539No Hit
AAAGCAGGCTGGTGCGGCAGGAGCACTAGTGCGTGGGCTGGGACTGGGGG47680.12022716074784924No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG47570.11994979103974808No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT46120.1162935539784146No Hit
CTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTCCC45600.11498235172193635No Hit
CTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTT45410.11450325858976163No Hit
GTGAACAGATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTG45180.11392330374555011No Hit
GTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAGTCTGT44640.11256167063305349No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC43700.110191420400189No Hit
GGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAA43650.11006534326014304No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA43140.10877935643167401No Hit
GTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAAA42810.10794724730737051No Hit
GAATACAGACTGAACTATGTATTGCTGTGCATGATGGAGCGCACTGGCAA42000.10590479763862559No Hit
CTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAG41900.10565264335853364No Hit
GAAGAGAGGAATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGA41380.10434144110205541No Hit
CAGATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGAT41190.10386234796988067No Hit
ATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAA40730.10270243828145763No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)