FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1098792
Sequences flagged as poor quality0
Sequence length151
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAGGGCAGCGAGGA83300.7581052646906785No Hit
GTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTC71090.6469832324953222No Hit
GGAATAAGCAGGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGG66110.6016607328775601No Hit
ATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAA44590.4058092887461867No Hit
GTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGC39530.35975871684540844No Hit
GTGTATGCACATCTCTGATAGCAGGGACAGGTTTGTGCATGTCTCAGGCA37210.33864462063793693No Hit
GTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTA36780.33473123211672456No Hit
GGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCAC34840.3170754792535803No Hit
GCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGC33850.3080655847512541No Hit
GGACAGATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCAC31770.2891357053928314No Hit
CTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATG30970.28185498256266883No Hit
CACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTGCCTGA27990.2547342900203132No Hit
GATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCACGTCTC26600.24208403410290574No Hit
GTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGT26530.2414469708552665No Hit
GGCTGGCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAG25580.23280111249444846No Hit
CTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGA25380.23098093178690782No Hit
CATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTAGACT25020.22770460651333466No Hit
GTCTCAGGCAGTGAGGAGAGGTGTATGCAGGTCTTTGATAGCAGGGACAG24470.22269910956759786No Hit
CTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCACGGCAACCT24410.22215305535533567No Hit
GCACACACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTA24280.22096993789543426No Hit
GGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTG23410.21305215181763246No Hit
GTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAG23300.21205105242848513No Hit
CCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGTCAGGGTTCA22670.20631748319973206No Hit
GGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGC22660.20622647416435502No Hit
CTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTCACTCTGTTAC22540.20513436573983065No Hit
GGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATT21410.194850344742226No Hit
TCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCA21160.1925751188578002No Hit
CCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAACC20660.18802466708894858No Hit
GGTGTATGCAGGTCTTTGATAGCAGGGACAGGTGTGTGCAGGTCTCAGGC20580.18729659480593233No Hit
GGTGTGTGCTGGTCTCAGGCAGGGGGACAGGTGCGTGCAGGTCTCAGGCA20420.1858404502398998No Hit
GTCTCACTCTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC20030.18229109786019557No Hit
GTCTAGGACCTACCCCTTATCTCTCTCTCTTGCTCCCACTGTCACTATGT19710.17937880872813053No Hit
GCACACACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTG19570.17810468223285206No Hit
CTTCCTGCCCTTGCCCTGGGTCAGGGTTCATCCTCCCAGCCCGATGTGCA19380.17637551056068845No Hit
CCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCAT19150.17428230274701673No Hit
CCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGT19130.17410028467626265No Hit
CATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAGTGCTCTCTGC18970.17264414011023013No Hit
ACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTCTCTATCA18890.1719160678272139No Hit
ACCCTGTCTCTACTAAAAATACAAAAATTAGGCGGGTGTGGTGGTCCACG18830.1713700136149517No Hit
GCATACACCTCTCCTCACTGCCTGAGACATGCACAAACCTGTCCCTGCTA18790.17100597747344357No Hit
GAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATCTC18330.16681956184610008No Hit
TCTCAGGAAGAGGGACAAGTGTATGCACATCTCTGATAGCAGGGACAGGT18250.16609148956308384No Hit
GGATAGGAGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGA18130.16499938113855944No Hit
CCATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAG17930.1631792004310188No Hit
GAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGCACAGCCACCA17530.1595388390159375No Hit
GATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATA17500.1592658119098064No Hit
CTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAG17280.15726361313151171No Hit
GGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAG17250.1569905860253806No Hit
AGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGAAAATGGT17210.15662654988387248No Hit
CACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAA17130.1558984776008562No Hit
GGGTAGGGTCCGCCATCAGGAGCTTAGTGGAGAGACCATTGTGCAACATG17110.15571645953010216No Hit
ATCTTGGCTCACGGCAACCTCTGCCTCCCAGGTTGAAGTGATTCTCCTCC16970.1544423330348237No Hit
GCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTG16780.15271316136266008No Hit
TTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTAT16730.15225811618577492No Hit
AAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCT16700.15198508907964384No Hit
GGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACT16250.1478896824876774No Hit
AGCCAGTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGG16190.14734362827541517No Hit
GGGTAACAGAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAA16070.1462515198508908No Hit
GTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCAC15970.14534142949712048No Hit
CGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAA15750.14333923071882576No Hit
ACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATG15750.14333923071882576No Hit
CTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTG15630.14224712229430136No Hit
CCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCG15580.1417920771174162No Hit
ATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTC15360.13978987833912151No Hit
GAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCC15050.13696859824243351No Hit
GCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGC15020.1366955711363024No Hit
GTTTGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTACTAAAAAT14760.13432933621649956No Hit
GCCTGAGACATGCACAAACCTGTCCCTGCTATCAGAGATGTGCATACACT14660.13341924586272924No Hit
GATAAGGGGTAGGTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTG14610.13296420068584408No Hit
ATCCAGAATGGAGTGCTCTCTGCTCTCACCAAGACCTGGCCTCTGCAGCA14520.1321451193674508No Hit
GAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGG14450.13150805611981156No Hit
CCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCAC14350.13059796576604124No Hit
TCTCTGATAGCAGGGACAGGTTTGTGCATGTCTCAGGCAGTGAGGAGAGG14080.1281407218108614No Hit
CCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGC13850.12604751399718964No Hit
AGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCC13690.12459136943115713No Hit
ACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCC13620.1239543061835179No Hit
GAGAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATC13420.12213412547597724No Hit
CTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGGG13390.12186109836984617No Hit
CTATCAGGCACATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAG13300.12104201705145287No Hit
AATTAGGCGGGTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAG13200.12013192669768254No Hit
GCTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGG13070.11894880923778112No Hit
GGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAG13010.11840275502551893No Hit
GGTTTGTGCATGTCTCAGGCAGTGAGGAGAGGTGTATGCAGGTCTTTGAT12960.11794770984863377No Hit
GGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCT12930.11767468274250267No Hit
GTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGG12800.11649156528260125No Hit
GGACAGGTGTGTGCTGGTCTCAGGCAGGGGGACAGGTGCGTGCAGGTCTC12410.112942212902897No Hit
ATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCG12360.11248716772601183No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC12330.11221414061988073No Hit
CCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAAGA12100.110120932806209No Hit
GCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAG12020.10939286052319273No Hit
GAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAAAGAGTCTA11920.10848277016942243No Hit
AGCGAAAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCA11810.10748167078027505No Hit
GGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTA11770.10711763463876693No Hit
CTCCATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAG11710.10657158042650475No Hit
GGCCAACATGGCGAAACCCTGTCTCTACTAAAAATACAAAAATTAGGCGG11650.10602552621424256No Hit
ATCAAAGACCTGCATACACCTCTCCTCACTGCCTGAGACATGCACAAACC11600.1055704810373574No Hit
ATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCC11510.10475139971896409No Hit
GGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCT11370.10347727322368565No Hit
GTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGT11260.1024761738345383No Hit
AACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGCGAAACCCT11200.10193011962227609No Hit
ATCAGGCACATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAGTG11180.10174810155152204No Hit
ATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTA11040.10047397505624359No Hit
GTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCACGTCTCAGGAA11000.10010993891473546No Hit
GTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTAGACTCTTT11000.10010993891473546No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)