FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5842899
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGT367170.6284038111903012No Hit
ATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAA354710.6070787805847748No Hit
GTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTA312780.5353164584908964No Hit
GATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATA308170.527426539462688No Hit
TTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGAC301850.5166099910335606No Hit
GTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGC281110.48111391280253174No Hit
CCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGA242570.4151535051350365No Hit
GCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTG207750.3555598000239265No Hit
GTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGTC184010.3149292842474258No Hit
TCCCTGTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTG181030.3098290762855904No Hit
CTTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAA179810.3077410716837652No Hit
GTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTCATACGTGAGAG178360.3052594268701205No Hit
TTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAAA174130.29801986993100515No Hit
ATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGACATGAT170830.2923719886309861No Hit
GTTGATTACCTGTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAA155030.2653306175581676No Hit
GCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTAC149080.25514731642631505No Hit
CATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAG144820.24785641511174505No Hit
ATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCT136560.2337195970698792No Hit
GTCTTAGAAGACAGAGATCATGTCTCTCTCCAGAGCACAAAGGCAGGTTT130240.22290304864075178No Hit
GTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGC127010.21737497088346042No Hit
ACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGG124510.21309627292890052No Hit
ATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAGCCTCT119770.204983861607055No Hit
GTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCACGTA118690.20313546409068511No Hit
AAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAG114770.19642646569793523No Hit
GACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATC112750.19296927775065084No Hit
CGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGCATTAAGACAGGATG111190.19029937022700547No Hit
GCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGCAAGCAAACCTG109230.1869448710306305No Hit
CTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAG107500.18398401204607506No Hit
TTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTC103700.17748039115514402No Hit
TATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGT103660.17741193198787109No Hit
ATCTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAG103280.176761569898778No Hit
GTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATT103060.1763850444787767No Hit
ATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAG101270.17332149674331185No Hit
CATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAA100440.17190096902239796No Hit
GCATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTG100240.17155867318603318No Hit
GGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAATCACCT99040.16950489816784442No Hit
GTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAATC88990.1523045323905137No Hit
CTGTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTAC85600.14650261796413047No Hit
TGCAGGGACAGGGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCA85140.14571533754049146No Hit
GTAGAAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACAT84310.1442948098195776No Hit
GACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTT82080.14047821124411017No Hit
TACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACA81970.14028994853410953No Hit
GGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCA80200.13726063038228112No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC79280.1356860695350031No Hit
TATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTT78580.1344880341077263No Hit
ATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAA78240.13390613118590616No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG78030.13354672055772313No Hit
GCTTAGCACAGGCAGACCCAGTCTTTCCTAGCAGCCTCTGCGCAAACCTA77200.13212619283680926No Hit
TAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTG76820.13147583074771615No Hit
TCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTC76750.13135602720498848No Hit
CCATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATC76180.1303804840713488No Hit
CCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCAC74600.127676346964067No Hit
CTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTT74490.12748808425406635No Hit
GTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATTTTGTTCTGAAT74210.12700887008315564No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA73080.12507489860769458No Hit
TTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGTGC73070.12505778381587632No Hit
ATGTTACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCG72820.12462991402042034No Hit
GAATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAA71890.12303823838132406No Hit
GGGTAATTCAGAACAAAATTTCACCAATGCCTGTGCTGGAAGGACATGCA71400.12219961358223032No Hit
TACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTG71110.12170328461950139No Hit
GGCACGGGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGC69880.11959816522585792No Hit
AACCTAACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGG69630.11917029543040193No Hit
TACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAG68090.11653461749039304No Hit
CACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCACAGGCAGACCCAGTCT67430.11540504123038922No Hit
TATTTATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCT65390.11191362369946836No Hit
GCTCTGGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAAT65210.11160555744674004No Hit
AACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGC65120.11145152432037589No Hit
CATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTGT63800.10919237180036828No Hit
GTGGAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGT63260.10826817304218334No Hit
GGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAGAGAACCC62910.10766915532854496No Hit
GACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGG61980.10607747968944868No Hit
CACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAAT61480.10522174009853671No Hit
GTTTTAACTCTTTTGGTCCCTCATACGTGAGAGGCTCTAACAGGTAATCA61000.10440023009126122No Hit
GGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATT59990.10267163611761901No Hit
GGATCTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAG59840.1024149142403454No Hit
GAATTACCCCTTCAAGGTGATTTTTAGCAAACAGTCTTAGAAGACAGAGA59580.10196992965307118No Hit
GTATTATATGGCATGAATATGGCACACTTTGTTTATCCATTCTCTAGTTG59100.10114841964579568No Hit
GGCAGACCCAGTCTTTCCTAGCAGCCTCTGCGCAAACCTAACCATGACTT58910.10082323860124914No Hit
GAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGTCCT58480.10008730255306483No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)