FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2999197
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC134110.4471530212920325No Hit
CCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAGGTGGACCTCTG106510.3551283893655535No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT87450.291578045723572No Hit
CCATAAGGGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAAT81100.27040571192889296No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA72680.24233153073972802No Hit
ATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCC68820.2294614191731987No Hit
CAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGGCTTATCAG68580.22866120498253364No Hit
GAGTAGCAGTGACGGGTATCTCCTATCATTAGAGGAATTCACATCCATAG67410.22476016080304162No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC65420.2181250514721107No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG64390.21469079890383994No Hit
CTCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCT60700.20238750572236502No Hit
GTCTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCA59770.199286675733538No Hit
ACCCAGAGGTGGTGGTACAGGTGGGAATAGTGACTCGGATAACTTGGACA59460.19825306573726234No Hit
GTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACG48850.16287693005827894No Hit
TCCTTGGACAAGCGCCTCAAACTCATCCACCTCTGCCCCACCAACTTTGG48400.161376528450782No Hit
ACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAG47550.15854243652551No Hit
CCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCAGA47440.15817567168812185No Hit
CTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAA45220.15077369042447028No Hit
ATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAA43580.14530556012159254No Hit
CCCAAAGGAAATGCATTTTTACTGGAAGCTGCTGTGGTGCCAACTCCAGA42490.1416712540056555No Hit
CCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCACA41730.1391372424018829No Hit
GAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGA41010.13673659982988778No Hit
ACCTATGGCAAGCAGTTTTCTGGATTTTTCTAAAGGTCTGCCAATTTCCA40270.13426927274200395No Hit
CTGCTACTCTCACTGTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACT38230.12746745212135116No Hit
GCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTA37950.1265338688989086No Hit
ATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGCTGGAAC37760.12590036599796545No Hit
GACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTT37190.12399985729513599No Hit
CCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA36820.12276619375119406No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG36650.12219937536613967No Hit
CCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTAT36370.12126579214369712No Hit
CCCCTGTGGGGACCAGTTTTGTTGCTCCTGTTGGACACAGTGGGATCTGG35390.11799825086514824No Hit
GGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGGGTCTCCT34860.11623111119409629No Hit
GTGCTAGGAGTTGTCACCACATTGGCCCTTTTTTTCCCAAACACATTTGT34540.11516415893987623No Hit
GTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTAC34440.11483073636043246No Hit
AACTTGGACAGCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAG34180.11396383765387869No Hit
ATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCA34000.11336367701087992No Hit
ATCCTGGAAACCACGGAAGCTGGAACGGACAACTTCTTTGACCTTCGTAC33880.11296356991554739No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA33840.11283020088376988No Hit
CATTGGAACAGAGAGGCTAGCCCGGATTCGGCAAGGAGGATCTGTCGCAC33260.11089634992299605No Hit
TCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCTC33210.11072963863327416No Hit
TCCTTTGGGTGCTCCAGCTCTCGTAACATCACAGGCGACAACATTATCAA33100.11036287379588604No Hit
GTGCAACATCACTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGG32750.10919589476783284No Hit
CATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTTTACCA32680.10896249896222222No Hit
ATTCCCACCTGTACCACCACCTCTGGGTTCTCCATTTATTTTATTTTTAT32210.10739541283883652No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT32110.10706199025939277No Hit
GTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAA32010.106728567679949No Hit
GGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAACAGTGA31830.10612840703695023No Hit
GCCATAGGTTGATGAAAAATGTTAGAGGGGGCTACCATTCCAGAATCATC31500.1050281125247858No Hit
ATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTC31110.10372776446495513No Hit
GTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAACTTGGAGTCCCAAC31060.10356105317523324No Hit
GTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGG31000.10336099962756698No Hit
CCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTGAGGATGGGAGGG30080.10029351189668435No Hit
GTTCCAAGCAGGATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTG30020.10009345834901809No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)