FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3240604
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGATAGATAGATCTTTACTCTGGACAGTTAGGTGGGAACAGTTTT74470.22980283922379902No Hit
ATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTCTT52700.16262400466085952No Hit
TGGTATTACTGTCCAACTGAAAGGAAACATACTCTTAGAAGTGTATTTTG50310.15524883632804257No Hit
GTGCAGGGGTTGATCCCCCGGACCCTGGGATCATGACCTGAGCCGAAAAG49310.15216299183732415No Hit
ATATTAATATGGCTACTCAAAGCAACTCACTAGTCAGCTAGACATTAGAT47590.1468553393132885No Hit
ATATTGTACCTTGTCATTTGCAAATAGTGACAGTTTTACTTTTTCCTACC46850.1445718143901569No Hit
ACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCC46250.14272030769572586No Hit
CTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTC45890.14160940367906724No Hit
CTATATTACCTGGCAAACAATATTTCGTGTTTCTAACTTATCTTTTCCTT43080.13293818066014854No Hit
GTCCTGAGGAGGGGAGGTGCTCAGGCCTTGTAGGTCTGGCCTTGAGGCCT40630.12537786165788847No Hit
GTCAATGACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTC39500.12189085738337668No Hit
ATTCCATAGTGTCTGAGAATGAGGGCCATACCCTGCTCCCTTAACCCAAA37350.11525629172833213No Hit
TATAAAAACCACTCAAGCTTTAGGTACTGCTATTAATGCTAGAAATAACT35860.11065838343716171No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT35380.1091771780816169No Hit
GGCTTAAAGATATGAAATGGAGTTATTTAATCCTCCCTCCTCAAAGTGCA34230.10562845691729074No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG33950.10476442045988957No Hit
GTGTGAGGCGTGACTGATTTGCAATTCAAAAAGCTCCAACAAAGGCAGAA33780.10423982689646745No Hit
GTGTAAGGGCTCTTTATTTCTCATTGCGATGATGTACTCGTTAGGAATAA33000.1018328681937071No Hit
AATGTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGG32730.10099969018121313No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA32420.10004307838909043No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)