FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences740348
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG37390.5050327683737919No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC36040.4867981003528071No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC34370.4642411406527741No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC29010.39184275502871624No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG27220.3676649359490402No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC25090.3388946819603754No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG21500.290403972186053No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA21410.28918832765132074No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA19650.26541572341655545No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAA18340.24772134185545178No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA16330.22057194724643003No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA16320.2204368756314598No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC15100.20395813860508843No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA14890.20112163469071304No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA14820.20017613338592122No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA14780.1996358469260402No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA14560.1966642713966945No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC13280.17937510468050158No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA13200.17829453176073953No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT13080.17667367238109646No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG12260.16559779995353538No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG12210.16492244187868407No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT12190.16465229864874356No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG12060.1628963676541302No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT11990.16195086634933842No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG11360.1534413546062122No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG11350.15330628299124197No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA11210.15141528038165836No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA11180.1510100655367476No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA10800.1458773441678778No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT10640.1437161983283537No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT10540.1423654821786511No Hit
TACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC10310.13925883503433523No Hit
CCCCTATGTCTCCCCATATCCCTTGTGAGGTTAGGTTTGTATACCCACAA9930.13412611366546542No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA9630.1300739652163577No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG9500.12831803422174437No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA9430.12737253291695258No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA9430.12737253291695258No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC9380.12669717484210127No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG9370.12656210322713102No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA9070.12250995477802332No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA8610.11629666048939148No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT8490.1146758011097484No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG8480.11454072949477813No Hit
GGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATATGCT8360.11291987011513505No Hit
GAATAAATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAA8290.11197436881034324No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT8220.11102886750555144No Hit
TCTTTATCCCGTCCCTCTGGGACACACGGATGGCTCCACTGGCTTTTCTA8160.1102184378157299No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA8130.10981322297081912No Hit
GTCCTGGACTGAGATCTGTCAGGTTTTGCCTGGGTCCCTTTGCACCACTG8100.10940800812590835No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG7990.10792222036123551No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG7880.1064364325965627No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC7880.1064364325965627No Hit
CTCCTTGGTTGACCTAGAAAAGCCAGTGGAGCCATCCGTGTGTCCCAGAG7450.10062835315284163No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)