FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_E10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1670763
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATG324821.9441416885578624No Hit
GTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAG132760.7946070148788308No Hit
GTATTAATCTCCTTTAAGTATTGCTTCTGAGTTTATATTTGGTTTGCTTG103630.6202555359437575No Hit
GTTAAATGATGATCTAAATCTTCAGAAGGTCACAGTACCTTTTGGAAGAC93830.5615997002567091No Hit
GTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAACC86800.5195231160852856No Hit
ATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAG85310.5106050349451119No Hit
CCTTTAAGTATTGCTTCTGAGTTTATATTTGGTTTGCTTGCTTGCAGCTT84540.5059963621411295No Hit
CCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTCAGAAGGTCACAG78770.47146124255804084No Hit
GCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAATCTTC70330.4209454003949094No Hit
GTGCATAATCAGTGAAATCTTGGAACACTGAAAAAATTAAAAAAGAAAAA68500.4099923208737565No Hit
ATTTTAGAGTAACAAGATATTAGACCCAGGTGACAGTCCTGAGATCTAAA66040.3952685090584362No Hit
ATATATGCCAAACAAACTTCTATTTTCTAAAGACTCTGGGATTGCTAAGT61720.3694120590412883No Hit
ATACTGTGTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGTTATGA60690.3632472110047924No Hit
ATATTAGACCCAGGTGACAGTCCTGAGATCTAAATTGTTGTGTTTCTATG60210.3603742721139982No Hit
GTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCT58160.3481044289345646No Hit
GTATTAAGGTGGGCACGTATTGCATGGAGCACTGGGTATGGTGCATAATC56520.3382885543910178No Hit
CTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAA53060.3175794532198762No Hit
GCATGATACTGTGTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGT52900.3166218069229448No Hit
GTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAA50500.30225711246897374No Hit
GGGTATGGTGCATAATCAGTGAAATCTTGGAACACTGAAAAAATTAAAAA45660.27328831198679887No Hit
GTTTTATTGCATAGAAACACAACAATTTAGATCTCAGGACTGTCACCTGG44490.26628552344048795No Hit
CACATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTTAGTTTC40820.24431951150462394No Hit
GTTATGAAGCTTATCAATTGTGTGGACAGACATCTGCATTGTTGCCAGTT39540.2366583411291727No Hit
TCCCAGAGCAGTGAGAAATAATGTTAACTTTGTCACAGTAAATGCGATTC37570.22486732109820484No Hit
TGTGTATACAGATTTTAATAGGCCTTCAGGAATTTTACATATCTATCATC37510.22450820373685557No Hit
ACCTATGCCTGCAAGGCCATCAGCGGGAGGAAACGAGGCAGCCGGAATCC36770.2200790896135478No Hit
GTAAAGGTATCTTTGATAACCTAAATTTGAAAATGAAAAGCTGCAAGCAA36510.2185229143810343No Hit
CTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGG36390.21780467965833572No Hit
TTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGATCTAAA35890.2148120349804251No Hit
ACTTAAAGGAGATTAATACATGTGGCAGATAAAAAAAATCTGGTTTCTAA35770.21409380025772654No Hit
CATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTTAGTTTCAT35140.21032306796355915No Hit
GTATCATGCAAAAACATGAATGAAACTAAAAACAATGTCAAGAGAAAAAA35090.21002380349576813No Hit
ATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAA35020.2096048332408606No Hit
ATCTAAATCTTCAGAAGGTCACAGTACCTTTTGGAAGACATACAGACATA35010.2095449803473024No Hit
TCCCAGGACCCTGGGATCATGACCTGAGCCAAAGGCAGACACTTAACAAC34920.2090063043052785No Hit
CTTAACAACTGAGCCACCCAGGGGCCCTGCTGCATTATTTTAGAGTAACA34670.20750998196632317No Hit
CTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAAT34670.20750998196632317No Hit
ACTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAA34590.2070311588178575No Hit
ACACAGTATCATGCAAAAACATGAATGAAACTAAAAACAATGTCAAGAGA32360.19368396355437606No Hit
GATTACAAGTAGGCAGAGAGGCAGACAGAGAGAGGAGGAAGCAGGCTCCC31900.19093073045069828No Hit
TTTAATGACTGAGCCACCAAGGCGCTCCTGTAGTTCCACTTAAATAAGAT31850.1906314659829072No Hit
GTTTTAAATGTATGTCTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGA31680.1896139667924176No Hit
CAATTGATAAGCTTCATAACAGCTGAAAATGGAAAACTACATAGGAAAAA31520.1886563204954862No Hit
CTGTAAAATAGTGAGTCACATGTGCGTTTCCAGGAATGAACAAGGAATAA31280.1872198510500891No Hit
GTATTTATGAGAATGTTGTTCCAGAATTTTCTAATGTAATTGCTTACTTC31150.18644176343383234No Hit
CAATAAAACCGTGTGGAAGACTGTGTTCAGTGGCATACACTAAATGCAGA31090.18608264607248307No Hit
CCTGTAGTTCCACTTAAATAAGATTCATAATTTTTGGCTGGCCAGTAAAG29650.17746382940010044No Hit
CTCCTGTAGTTCCACTTAAATAAGATTCATAATTTTTGGCTGGCCAGTAA29380.1758478012740287No Hit
CATAAATACTGAGGTCTGGTTGGAATCGCATTTACTGTGACAAAGTTAAC29290.17530912523200476No Hit
TATCAATTGTGTGGACAGACATCTGCATTGTTGCCAGTTTCTGGCTATAC29010.17363324421237483No Hit
GTCTGAAGTAAGCAATTACATTAGAAAATTCTGGAACAACATTCTCATAA28810.17243618634121058No Hit
GTATTTGAAATTAGTAGGCAATTTCAACCCACCTACAGATGCTTTCTGCA28790.17231648055409415No Hit
ACTCTACTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAG28380.16986251191820742No Hit
AAATTATATATGCCAAACAAACTTCTATTTTCTAAAGACTCTGGGATTGC28270.1692041300890671No Hit
TCATTTAACTGCTAACTTTAAAGGCTTGAATAATGCTATAAAACTCTTAC28150.16848589536636854No Hit
GTATTGCATGGAGCACTGGGTATGGTGCATAATCAGTGAAATCTTGGAAC28020.16770780775011176No Hit
CTTCATAACAGCTGAAAATGGAAAACTACATAGGAAAAATAAAACACAGT27780.16627133830471466No Hit
GTCATTAAATGTCTGTCTTTGGCTCAGGTCACGTTCCCGGGGTCCTGGAA27670.16561295647557434No Hit
AAATATATGTGGTTAGAAACCAGATTTTTTTTATCTGCCACATGTATTAA27650.1654932506884579No Hit
TATATGTGGTTAGAAACCAGATTTTTTTTATCTGCCACATGTATTAATCT27440.16423633992373546No Hit
GTGAGAGAGGGAACCCAAGCAGGGGGAATGGGAGAGGGAGAAACATGCTT27020.1617225183942905No Hit
GTTTTTAGTTTCATTCATGTTTTTGCATGATACTGTGTTTTATTTTTCCT26880.16088457788447555No Hit
CATACAGACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCATT26780.16028604894889342No Hit
GTGTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGTTATGAAGCTT26590.15914884397128737No Hit
GTCCTGAGATCTAAATTGTTGTGTTTCTATGCAATAAAACCGTGTGGAAG26160.15657516954828424No Hit
CCTCTATGCTAAGCCAGGCACCCGTCGCAACATGCAAACACAGTACCTTC25910.1550788472093289No Hit
ATTTAGTATTTCAGACCCAATCTTCTCCCCAACCCTGCAAAGGAAACCAA25620.15334311329614075No Hit
GCATTTAGTGTATGCCACTGAACACAGTCTTCCACACGGTTTTATTGCAT25570.15304384882834968No Hit
ATTGATAAGCTTCATAACAGCTGAAAATGGAAAACTACATAGGAAAAATA24890.14897385206639122No Hit
ATGTATGTCTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGAAGATTTA24710.14789649998234342No Hit
GTATGGTGCATAATCAGTGAAATCTTGGAACACTGAAAAAATTAAAAAAG24430.14622061896271346No Hit
GTTTTATTTTTCCTATGTAGTTTTCCATTTTCAGCTGTTATGAAGCTTAT24400.1460410602820388No Hit
GGAATAACCAGCCAAACAAGTTTTAAATGTATGTCTGTATGTCTTCCAAA24300.14544253134645668No Hit
GATTAATACATGTGGCAGATAAAAAAAATCTGGTTTCTAACCACATATAT24120.14436517926240885No Hit
ATGCTAAGCCAGGCACCCGTCGCAACATGCAAACACAGTACCTTCTAATT24100.14424547347529243No Hit
GTGTTACGCAGTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGC24060.14400606190105958No Hit
GTGTATGTGTATACAGATTTTAATAGGCCTTCAGGAATTTTACATATCTA24050.14394620900750138No Hit
CACACATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTTAGTT23590.14119297590382357No Hit
GACATACAGACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCA23490.14059444696824147No Hit
CAGTAAAGGTATCTTTGATAACCTAAATTTGAAAATGAAAAGCTGCAAGC23450.14035503539400862No Hit
GTTAATGACTCTACTGTAAGAGTTTTATAGCATTATTCAAGCCTTTAAAG22800.13646459731272478No Hit
GTCTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGAAGATTTAGATCAT22510.13472886339953663No Hit
CCGTACATCGTTCACATCTGCTACTCACCACCAACTCGACGTCTCCTCCT22480.13454930471886198No Hit
GTTTATATTTGGTTTGCTTGCTTGCAGCTTTTCATTTTCAAATTTAGGTT22440.13430989314462913No Hit
GATTTTAATAGGCCTTCAGGAATTTTACATATCTATCATCAGGATGTATT22420.1341901873575127No Hit
GACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCATTCCTGGA22230.13305298237990668No Hit
CTCTTACAGTAGAGTCATTAACTAAAAAGAACAGAGGGTTGCATTTTTCT21780.1303596021697871No Hit
TCACAGTACCTTTTGGAAGACATACAGACATACATTTAAAACTTGTTTGG21700.1298807790213214No Hit
AGTAAGCACCGTACATCGTTCACATCTGCTACTCACCACCAACTCGACGT21620.1294019558728557No Hit
CAAGTAGGCAGAGAGGCAGACAGAGAGAGGAGGAAGCAGGCTCCCTGCCG21440.1283246037888079No Hit
CAGTGAGAGAGGGAACCCAAGCAGGGGGAATGGGAGAGGGAGAAACATGC21340.12772607485322574No Hit
GCATTACTTGTATAGCCAGAAACTGGCAACAATGCAGATGTCTGTCCACA20950.12539181200445546No Hit
GTTAGAAACCAGATTTTTTTTATCTGCCACATGTATTAATCTCCTTTAAG20890.12503269464310618No Hit
CTTTAAAGGCTTGAATAATGCTATAAAACTCTTACAGTAGAGTCATTAAC20870.12491298885598974No Hit
CTACAGGAGCGCCTTGGTGGCTCAGTCATTAAATGTCTGTCTTTGGCTCA20810.12455387149464046No Hit
CACACACACATATGGAAGCACAAATTTTTTTTTCTCTTGACATTGTTTTT20390.12204004996519555No Hit
ATAAAACACAGTATCATGCAAAAACATGAATGAAACTAAAAACAATGTCA20320.12162107971028806No Hit
CTGTAGTTCCACTTAAATAAGATTCATAATTTTTGGCTGGCCAGTAAAGG20040.1199451986906581No Hit
GCATTATTTTAGAGTAACAAGATATTAGACCCAGGTGACAGTCCTGAGAT20010.11976564000998347No Hit
CTATAAAACTCTTACAGTAGAGTCATTAACTAAAAAGAACAGAGGGTTGC19980.11958608132930883No Hit
GCTCTGGGACACTCATTCTCTGGACATGAGTCCTGAGGCTGCACGCAAGT19900.11910725818084314No Hit
GTATTAGATACATGTAATTAAAAACAAATAAACAAACAAAAAAAAACAAA19620.11743137716121319No Hit
ATCTAATACCTTCAGTGCTTTAAATATATAATTGCCTCCATAAGAAGATC19370.11593505482225787No Hit
GTTAAAGCATCAGTCACAAGGGAAAATGAGCTTTTTCCGCAGCGTGAAGG19360.11587520192869963No Hit
TTTCAAATACTGTATAATTTAGTATTTCAGACCCAATCTTCTCCCCAACC19350.11581534903514143No Hit
GAACTACAGGAGCGCCTTGGTGGCTCAGTCATTAAATGTCTGTCTTTGGC19100.11431902669618611No Hit
ACATACAGACATACATTTAAAACTTGTTTGGCTGGTTATTCCTTGTTCAT18940.11336138039925471No Hit
GTCTAATATCTTGTTACTCTAAAATAATGCAGCAGGGCCCCTGGGTGGCT18900.11312196882502187No Hit
CTGTATGTCTTCCAAAAGGTACTGTGACCTTCTGAAGATTTAGATCATCA18740.11216432252809047No Hit
GCATAGAGGAGGAGACGTCGAGTTGGTGGTGAGTAGCAGATGTGAACGAT18730.11210446963453224No Hit
TCTTGGAACACTGAAAAAATTAAAAAAGAAAAAAAACCCCAGAATTTCAT18550.11102711755048443No Hit
ATTTTTATCTCTGCTGCTGTTTCAAACGGATAGTTATCTTTGCTTCCCAG18310.10959064810508731No Hit
CTCTGGGATTGCTAAGTCTGAAGTAAGCAATTACATTAGAAAATTCTGGA18240.10917167785017984No Hit
GAGTTTATATTTGGTTTGCTTGCTTGCAGCTTTTCATTTTCAAATTTAGG18210.1089921191695052No Hit
ATGCAAACACAGTACCTTCTAATTTGTAACCTGCTTCCTCTGCATTTAGT18100.10833373734036485No Hit
TCTCAGGACTGTCACCTGGGTCTAATATCTTGTTACTCTAAAATAATGCA18090.10827388444680663No Hit
CCTTAATACCCACCACCAGTGTCACCCATCCTCCCACCCGTCTCCTCTCC18030.10791476708545736No Hit
TGCAAACACAGTACCTTCTAATTTGTAACCTGCTTCCTCTGCATTTAGTG18020.10785491419189916No Hit
GAGTTTTATAGCATTATTCAAGCCTTTAAAGTTAGCAGTTAAATGATGAT17830.10671770921429312No Hit
ATACAAGTAATGCTGCTATGAAATTCTGGGGTTTTTTTTCTTTTTTAATT17740.1061790331722692No Hit
GATCTAAATTGTTGTGTTTCTATGCAATAAAACCGTGTGGAAGACTGTGT17690.10587976870447813No Hit
TACCTTTACTGGCCAGCCAAAAATTATGAATCTTATTTAAGTGGAACTAC17610.10540094555601244No Hit
ATGCAGATGTCTGTCCACACAATTGATAAGCTTCATAACAGCTGAAAATG17460.10450315215263925No Hit
GTCTTCCACACGGTTTTATTGCATAGAAACACAACAATTTAGATCTCAGG17360.10390462321705712No Hit
ACTTGATACAGTAATTTTAAAAAAAAGGAAGACCTATGCCTGCAAGGCCA17200.10294697692012572No Hit
GTGTTTGCATGTTGCGACGGGTGCCTGGCTTAGCATAGAGGAGGAGACGT17200.10294697692012572No Hit
AAATAAAACACAGTATCATGCAAAAACATGAATGAAACTAAAAACAATGT17160.10270756534589286No Hit
ATCATGACCTGAGCCAAAGGCAGACACTTAACAACTGAGCCACCCAGGGG17050.10204918351675253No Hit
TCTCTAACCTACTTGATACCTGGGAAGCAAAGATAACTATCCGTTTGAAA16900.10115139011337934No Hit
CTGTAAGTACTATATAATGTCTCCTGTAAAATAGTGAGTCACATGTGCGT16770.10037330249712258No Hit
ATCTTTGATAACCTAAATTTGAAAATGAAAAGCTGCAAGCAAGCAAACCA16760.10031344960356436No Hit
GTGAGAAATAATGTTAACTTTGTCACAGTAAATGCGATTCCAACCAGACC16730.10013389092288973No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)