FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5442519
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAAGACTGAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCC249110.45771085043524884No Hit
AAGCAGGACAGTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGAC247320.454421932197205No Hit
CTCATATACTGCACCCATTTCAGCAGCAAATCCTGTTGCCCCTACTTTCA156450.28745880354299175No Hit
GTATATGAGAAGAAAAAAGAAATGCAGAATAACACAAAGATTCTAGACAA151280.27795952572696575No Hit
GTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCA134540.2472017093555392No Hit
CCCTCATACTGCAGCTTCTGTTTCCCTGCAGGCATACCCGTGGCTTCGTG114330.21006816880198304No Hit
GTGGGAGACAGGAAACGGCAAAAACGGCAGTGGCTGAGACCTGACAGCAA113970.2094067103853932No Hit
AAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATA102850.18897499485072997No Hit
GTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAATGGAAAC101950.1873213488092554No Hit
GTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGAA100980.18553908585344397No Hit
GTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGT99590.18298512141161105No Hit
GACCAGGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCC94580.17377982511406942No Hit
CTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTAATAAG94220.1731183666974796No Hit
GTATGAGGGCATTTTCATCAAGGATTCCAACTCATTGGCTTATTACAACA87380.16055065678227307No Hit
ACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACACACGGT85300.15672889704197634No Hit
GGCTGAGACCTGACAGCAAGTTTCTCTTCTCTACTTCTTCCGCCCGCCTC80000.1469907592421818No Hit
GTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATACT79790.14660490849917107No Hit
GTAGAAACAAGGGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAG73160.13442304932697524No Hit
GGCATACCCGTGGCTTCGTGAATCTTCACCTTGATGACAGAGACCTGGTC72750.13366972168585908No Hit
CCATAAAATTCGCCCTTTTAGAACTGTACAACTCAGTGATTTTAGTCTTT71110.13065641112139434No Hit
ATTCTAGACAATGAGCTTTTTCTTTCCAGACACAGGTGCTGCTCCTTCTT66410.12202070401591616No Hit
GTCTAGAATCTTTGTGTTATTCTGCATTTCTTTTTTCTTCTCATATACTG64580.11865829039825126No Hit
TGTTGTAATAAGCCAATGAGTTGGAATCCTTGATGAAAATGCCCTCATAC63440.11656367207905016No Hit
GACAGGTGCTGGTCTTCACACTCCCGCTCACAGACCAGGTCTCTGTCATC62220.11432206300060689No Hit
CTCTTAATCTTGGCTTAAATAAATAGGGTGCTTATTTTTCTTCTGTAAGA61150.1123560615957427No Hit
GAATGGAAACTGAACGGACAGGTGCTGGTCTTCACACTCCCGCTCACAGA60180.11057379863993126No Hit
ATAACATACTAAGGATTTCTGAATGGAAAGGCAGAAATAGCCTCCTGGCT60020.1102798171214469No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA59960.11016957405201526No Hit
ATAATAACCTAGGAGACACCAGTGGCTTGGACCAGCATAGCTGAAGCACA59830.10993071406824671No Hit
GGGTATGCCTGCAGGGAAACAGAAGCTGCAGTATGAGGGCATTTTCATCA59630.10956323717014126No Hit
GACTATAGGCACGCCAGAGTGGGAGCAGGGAGAGGAAACATTGGCCATTA59500.10932437718637271No Hit
GTATGTTATTGTCCTCATGCTTGTCTCTGTAGGTTACCAAATACCTGTTC59190.10875478799430925No Hit
CCTCTCTCCTTGAGGGCCAGGTGGATGATGGCGCCGTTGGCCATGTTGTA58400.10730325424679271No Hit
GGTCCAGTGTCCATCAAAGTCCAAGTGCCCAACATGCAGGATAAGACTGA56960.10465742058043342No Hit
GGGTATATGGAATCTATATCAAACAAGAAAGGCAGGAGGAAGATAGAAGC56630.10405108369855942No Hit
GTTCTAAAAGGGCGAATTTTATGGTATGTGAATTATGTCTTAATAAAAAA56380.10359173757592763No Hit
TCACACTCCCGCTCACAGACCAGGTCTCTGTCATCAAGGTGAAGATTCAC56050.10298540069405362No Hit
GGTCTCTGTCATCAAGGTGAAGATTCACGAAGCCACGGGTATGCCTGCAG54910.10089078237485252No Hit
GAGTTGGAATCCTTGATGAAAATGCCCTCATACTGCAGCTTCTGTTTCCC54760.10061517470127343No Hit
TCTCTACTTCTTCCGCCCGCCTCTCTCCTTGAGGGCCAGGTGGATGATGG54660.1004314362522207No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)