FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences879024
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCAT169071.9233832068293926No Hit
ATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGC134851.5340878064762737No Hit
GTATAAAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTT117361.3351171299077158No Hit
GTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTA104231.185746919310508No Hit
AGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACAT98261.1178306849414805No Hit
GTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCT74910.8521951619068422No Hit
ATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAA74540.8479859480514752No Hit
GTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGCTG73810.839681282877373No Hit
CTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTT71090.808737872913595No Hit
ATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAA66960.7617539452847705No Hit
CACACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGG64230.7306967727843608No Hit
CACACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCT64090.7291040972715194No Hit
TCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTC51490.585763301115783No Hit
CACACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCC46050.5238764811882269No Hit
GTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTG44640.5078359635231802No Hit
GAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACA42780.48667613170971447No Hit
ATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGATCTGCAGATTCT40800.46415114945667013No Hit
GTATCAGACTTCCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCC40210.4574391597954095No Hit
ACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGC39790.4526611332568849No Hit
ACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGG39330.44742805657183427No Hit
GCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCA38800.44139864213036273No Hit
CCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAAT38280.43548299022552284No Hit
CTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTC37850.4305912011503668No Hit
TGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATG35820.4074974062141648No Hit
GAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAG35260.4011267041627987No Hit
CCAGTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTG32990.3753026083474399No Hit
GTACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAA32230.36665665556344307No Hit
ATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTAT32180.3660878428802854No Hit
CTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGT31510.35846575292597244No Hit
ACACTGTTGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAA30180.34333533555397805No Hit
AGCGAAAGCAGGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCC28650.3259296674493529No Hit
GTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGACT28430.3234268916434591No Hit
CCTTTTACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCC27860.31694242705546155No Hit
ACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTA27540.31330202588325234No Hit
GGGCAAGAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGC27070.30795518666157007No Hit
GGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATT26290.29908170880431023No Hit
ATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCA25790.2933935819727334No Hit
ACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCA25770.29316605689947034No Hit
CTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATA25520.2903219934836819No Hit
CTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAA24610.27996960265021203No Hit
AGCGAAAGCAGGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGA24450.27814940206410743No Hit
GGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGT24370.27723930177105516No Hit
TCCGTATCCTAATACTTTATACGACGAGACTTTAAAATCAAGCTCCTGAG24330.276784251624529No Hit
GGTCAATGATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGA23820.27098236225632066No Hit
ATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGT23760.2702997870365314No Hit
GTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGGCAGTGGCTCAG23160.26347403483863924No Hit
TTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTA23000.26165383425253463No Hit
CTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCT22650.2576721454704309No Hit
GTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGA22530.2563069950308524No Hit
GAGAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTT22160.25209778117548554No Hit
TGTTGGTACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAA21790.24788856732011869No Hit
ATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTAT21730.24720599210032945No Hit
AAGTATTAGGATACGGATCTGCAGATTCTCCAGTACACTGTTGTTGGTAC21650.24629589180727718No Hit
GAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCGTGCTCGACCCGCCC21320.24254172809843644No Hit
ATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTATAC21120.2402664773658057No Hit
GAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATA20760.2361710260470704No Hit
GCCCAAGGCTTCGGCCTGATTGTTCGATTCGAAATGCTGAACGCCGGGAC20630.2346921130708604No Hit
AAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCGGCCC19600.22297457179781213No Hit
ACTATAAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAG19440.22115437121170753No Hit
GCCCAATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAA18940.21546624438013068No Hit
GGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGG18900.2150111942336045No Hit
GTACCTGAACCCAGGACTCCAGGATCAAGACCTGAGCTGAAGGCAGATGC18860.2145561440870784No Hit
GTTTATGTAGTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATA18850.21444238155044684No Hit
ACATAAACTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATT18840.21432861901381534No Hit
GATCTGCAGATTCTCCAGTACACTGTTGTTGGTACTATAAATTGGTGAAC18800.21387356886728917No Hit
TTTCCAAAGTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTA18300.20818544203571235No Hit
TTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAA17270.19646790076266407No Hit
GGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAGCAGGGGAAGTC17200.19567156300624328No Hit
GTGGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAA17020.19362383734687563No Hit
AGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAA16690.18986967363803492No Hit
GATTTGTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCAT16230.18463659695298423No Hit
GTTAAGCATCTGCCTTCAGCTCAGGTCTTGATCCTGGAGTCCTGGGTTCA15880.18065490817088045No Hit
ATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCATGAAATCGAGT15850.18031362056098582No Hit
TCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAG15410.1753080689491982No Hit
GCTCAGTGAGGTTGCAAGCACGCTGCAGCTGACGATGGCGAAGGTTCTGG15200.17291905567993593No Hit
CACAGAGGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCC14990.17053004241067365No Hit
GGGCACAAGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAG14990.17053004241067365No Hit
GCCTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCT14460.1645006279692022No Hit
GTATATTTGTTCGTAGCGCGGAGTGCGTGTAAAAGGAGCGCTTCAGCGTG14420.16404557782267606No Hit
GTTCCAGAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTT14240.1619978521633084No Hit
CACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTA14220.16177032709004532No Hit
GTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCC14070.16006388904057225No Hit
AGCAGGAACAGGAGAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACA14000.15926755128415152No Hit
GAGCTGAAGGCAGTGGCTCAGTCAGTTAAGCATCTGCCTTCAGCTCAGGT13980.15904002621088845No Hit
GTTCTGGCTCGAGGCAGCACGCTGAAGCGCTCCTTTTACACGCACTCCGC13930.15847121352773078No Hit
AGCGAAAGCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCG13890.1580161633812046No Hit
ACTCCACAGTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCA13830.15733358816141538No Hit
GAAAGAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGC13810.15710606308815234No Hit
AGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGCACGTCG13560.1542619996723639No Hit
AATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCC13210.15028031089026012No Hit
CAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATA13160.14971149820710242No Hit
GTTCATAACTCAAACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGG13160.14971149820710242No Hit
CTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTTAGC13160.14971149820710242No Hit
GAGTATGTGGTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATA13040.14834634776752398No Hit
CTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTGGTTTGAGTTATGA12810.14572980942499864No Hit
CTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAGGACTCGGA12750.1450472342052094No Hit
GAAAAGAAAATGTATAGGAGTTTATGTAGTTCCAGAAGAGAATTGCTGGA12660.1440233713755256No Hit
ATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAGAGGG12600.14334079615573636No Hit
AACCAAAGGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTA12580.1431132710824733No Hit
TTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTT12540.14265822093594713No Hit
CTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATATGATCCAGCA12520.14243069586268406No Hit
CTTCAGCTCTGGTCATGAAATCGAGTCCTGTATCAGACTTCCCCTGCTCT12380.14083802034984255No Hit
GAGAGGAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGT12350.14049673273994795No Hit
GGCAGACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTC12180.1385627696172118No Hit
CTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGTATGCAATA12140.13810771947068567No Hit
GAACAGAGGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAAC12120.13788019439742258No Hit
GTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACGAGA11880.1351498935182657No Hit
CTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGA11870.1350361309816342No Hit
GCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCAGACACTT11850.1348086059083711No Hit
TATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAG11770.1338985056153188No Hit
GAAGAGAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGT11610.13207830502921422No Hit
GTGAGCAGGGAGTCCAACACGGTACCTGAACCCAGGACTCCAGGATCAAG11400.12968929175995195No Hit
GATTATATTTATTTATTTGACACACACACACAGAGGGCACAAGCAGGCAG11370.12934800415005734No Hit
GTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAAGAAA11220.12764156610058427No Hit
TTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCCAAA11220.12764156610058427No Hit
GTATGCAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTAT11030.12548007790458507No Hit
CTCCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTA10880.12377363985511204No Hit
ACAGTGTACTGGAGAATCTGCAGATCCGTATCCTAATACTTTATACGACG10840.12331858970858589No Hit
TACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAATTTATA10810.12297730209869127No Hit
CCCCTGCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTT10680.12149838912248129No Hit
ATGCGGGGCTCTGTTCTAGGACTCGGAGTTCATAACTCAAACCAAAGGCA10610.12070205136606055No Hit
ACCACATACTCAACTTTAGGAGTTTTCCAAAGTGGTAACAAGTTCACCAA10500.11945066346311364No Hit
GAGAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCT10380.1180855130235352No Hit
GGGAAAGGGAGAAGGAGACTCCACAGTGAGCAGGGAGTCCAACACGGTAC10290.11706165019385137No Hit
TCCTAGAACAGAGCCCCGCATTGGGCTTCCTGCTCAGCAGGGAGTCTGCT10260.11672036258395675No Hit
ACACTTAGCTGACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGT10260.11672036258395675No Hit
GGTAACAAGTTCACCAATTTATAGTACCAACAACAGTGTACTGGAGAATC10190.11592402482753598No Hit
AAATTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGT10170.11569649975427292No Hit
AGCAGGCAGAGGGAGATCGAGAAGAAGCAGGCTCCCCGCAGAGCAGGGGA10130.11524144960774678No Hit
CTTTAAAATCAAGCTCCTGAGTGGCTCAGTCAGCTAAGTGTCTGCCTTTG10110.1150139245344837No Hit
GTTACGAGTGAAGGGCATCGGAGTACGAGGCTTGAAGAAGCTCATGCCGC10110.1150139245344837No Hit
GAAGCAGACTCCCTGCTGAGCAGGAAGCCCAATGCGGGGCTCTGTTCTAG10020.11399006170479987No Hit
CTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATTAGGATACGGA9800.11148728589890607No Hit
GCTCTGCGGGGAGCCTGCTTCTTCTCGATCTCCCTCTGCCTGCTTGTGCC9780.111259760825643No Hit
GAGCAGGGGAAGTCTGATACAGGACTCGATTTCATGACCAGAGCTGAAGG9680.11012213545932763No Hit
GACTGAGCCACTCAGGAGCTTGATTTTAAAGTCTCGTCGTATAAAGTATT9260.10534410892080308No Hit
CTCTGTGTGTGTGTGTCAAATAAATAAATATAATCTTTAAAAAAAGGAAA9240.10511658384754No Hit
CCAATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTAT9230.10500282131090846No Hit
GCTGAAGGCAGATGCTTAACTGACTGAGCCACTGCCTTCAGCTCTGGTCA9200.10466153370101385No Hit
GCAGGGAGGCTCGCAGCTTGCTGGCGGGTGGATCGAGCGGTCGGCCCGCG9050.1029550956515408No Hit
TTGGTGAACTTGTTACCACTTTGGAAAACTCCTAAAGTTGAGTATGTGGT9030.10272757057827774No Hit
CTCCAGGATCAAGACCTGAGCTGAAGGCAGATGCTTAACTGACTGAGCCA9010.10250004550501465No Hit
GAATTGCTGGATCATATTGCATACCACATACTCAACTTTAGGAGTTTTCC9010.10250004550501465No Hit
TACACGCACTCCGCGCTACGAACAAATATACCGGCGGGCTGCTCCGAGCG9000.10238628296838312No Hit
CCTATACATTTTCTTTTCTTTCTTTCCTTTTTTTAAAGATTATATTTATT8990.10227252043175157No Hit
ATTTATAGTACCAACAACAGTGTACTGGAGAATCTGCAGATCCGTATCCT8930.10158994521196237No Hit
CAATATGATCCAGCAATTCTCTTCTGGAACTACATAAACTCCTATACATT8830.10045231984564698No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)