FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D8.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D8.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4540702
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTT310870.6846298215562263No Hit
ATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGG273500.6023297719163249No Hit
GAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCG268190.590635544900326No Hit
CACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAG247760.5456425019743644No Hit
ATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTC239640.527759804541236No Hit
GTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCA237070.5220998867575982No Hit
GCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTC235120.5178053966104801No Hit
AGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCT223440.4920825017805617No Hit
GGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGAC222320.48961592282426813No Hit
CTCCAGGATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCA221080.48688506755122885No Hit
GTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGG212850.46876011682775043No Hit
ATCATGACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGT212360.467680988534372No Hit
GGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTT204370.45008459044438504No Hit
GTCCTGGGACCGAGTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGC201620.4440282581856286No Hit
CTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAG199630.4396456759329284No Hit
ACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAAAGGCA196110.4318935706417202No Hit
GAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGG187920.4138567120238236No Hit
GACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCA171890.3785538007118723No Hit
GGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCA168330.37071360331508213No Hit
GTCCTACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACC166430.36652922829994133No Hit
CCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCA164000.3611776328858401No Hit
GCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCA147350.32450929393736916No Hit
GAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGC147100.32395871827748224No Hit
CACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGA142520.3138721721883533No Hit
GAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAG141950.31261685968381103No Hit
GTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACT141570.31177998468078283No Hit
GAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCC140200.30876283006460237No Hit
TCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGA134240.2956371063328974No Hit
CGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCTC134020.29515259975219693No Hit
GGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTT130210.28676182669551975No Hit
ATTTATTTGAGAGAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAG126520.2786353299555884No Hit
ATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCC124030.2731515963831143No Hit
CTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAG122210.2691434055791373No Hit
GCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTC120870.26619232004214327No Hit
AAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGT118890.26183176081583864No Hit
GCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAA114190.2514809384099639No Hit
ATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCTCAGCAGG114100.2512827311724046No Hit
TTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAA113640.2502696719582126No Hit
TAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTAT104330.22976623438402255No Hit
AGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGA102330.22536162910492694No Hit
CTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAATTTAAAAAAA101800.2241944087059666No Hit
GGTTAAGCCGCTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGT94930.2090645895722732No Hit
GCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCTCATTTC93040.20490223758352785No Hit
AGCGAAAGCAGGGATAGCAAAGCTCACAAGAACGTGTCAGGGAAAACTGG92910.2046159382403866No Hit
GTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATC90640.1996167112486131No Hit
GGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAGGATCCTTGCT89670.19748047768825172No Hit
AGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTG89310.1966876487380145No Hit
CATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGA88350.19457343820404863No Hit
GGCAGAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGG86400.1902789480569304No Hit
GCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTT86260.18997062568739373No Hit
AAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCCCTGC83280.18340776382154125No Hit
GCACCTGGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATG82710.182152451316999No Hit
AGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAG81740.18001621775663762No Hit
CTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGGACTCCAG80360.17697704011406165No Hit
GGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGG79250.17453248418416362No Hit
ACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAAAAGCTCACAG77540.17076654667053684No Hit
GAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCA75780.16689049402493272No Hit
GAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGT75460.1661857571802774No Hit
CCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCA75320.16587743481074071No Hit
GCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGTCATGATCT75290.16581136573155428No Hit
GAGAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGA73100.1609883229509446No Hit
TGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAA72330.1592925499184928No Hit
GTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAA70160.15451355319067406No Hit
TCCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAA69990.15413916174195091No Hit
CCTCTGACCTCTCCATTCTCACACTCTCTCTCTCAAATAAATAAATAAAA69230.15246541173589456No Hit
GCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTC68580.1510339150201885No Hit
GGGTGGCTCAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTG68280.15037322422832416No Hit
CTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATC67990.1497345564628553No Hit
GGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGACGT66380.14618884921318331No Hit
GGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATC65260.1437222702568898No Hit
AAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCT65070.1433038327553757No Hit
GAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTGAGATCATGAC64570.1422026814356018No Hit
GAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCC64080.14112355314222338No Hit
GTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTGGGACCG62570.1377980761565062No Hit
GAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTG61840.1361903952296363No Hit
GAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGC61780.13605825707126343No Hit
GAAAGAAAGAAAGAAATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCC61220.13482496759311666No Hit
GAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTG58690.1292531419150607No Hit
AGCGAAAGCAGGCATAGTGGCAGGCAGAAGCAGAGGGAGACATAGGCTCC58550.12894481954552403No Hit
GATCATGACCCGAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCC58480.12879065836075568No Hit
AATGAGGGGCGCCTGGGTGGCTCAGTTGGTTAAGCCGCTGCAGCTCGGGT57980.12768950704098175No Hit
GTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGCA57850.12740320769784055No Hit
GGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTTTCTTTCTTTC57410.12643419453643953No Hit
GCAAAGCTCACAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGT56780.1250467438735244No Hit
ACGTCAGGCTCCCTGTTCGGCGGGGAGTCTGCGCTTCCTCTGACCTCTCC56440.12429796097607815No Hit
GCGCAGACTCCCCGCCGAACAGGGAGCCTGACGTAGGACTCGGTCCCAGG56150.1236592932106093No Hit
GAGAGTGTGAGAATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAAC54770.1206201155680333No Hit
GAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGC54740.12055404648884688No Hit
CAGTTGGTTGAGCAACTGCCTTTGGCTCAGGTCATGATCCTGGAGTCCTG53840.11857197411325386No Hit
CTCCATCTCCAGACCCTGAGATCATGACCCGAGCTGCAGCGGCTTAACCA52000.11451973725648588No Hit
AACTGGCTGTGAGCTTTTATGTCAGTCAAGAAAGCAGAAATGAGGGGCAC51590.11361679317427129No Hit
GAAGCAGAGGGAGACATAGGCTCCCTGCTGAGCAAGGATCCTCTGGTGGG51510.11344060896310747No Hit
AGTCAAGAAAGCAGAAATGAGGGGCACCTGGGTGGCTCAGTTGGTTGAGC51340.11306621751438434No Hit
GAGCTGCAGCGGCTTAACCAACTGAGCCACCCAGGCGCCCCTCATTTCTT51200.11275789514484764No Hit
CAACTGAGCCACCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAA49580.10919016486878019No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA48160.10606289512062232No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG48050.10582064183027205No Hit
CTCTCTCTCAAATAAATAAATAAAATTTAAAAAAAAAAAAAGAAAGAAAG48000.10571052669829466No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA47790.10524804314398964No Hit
GGCTCAGGTCATGATCCTGGAGTCCTGGGACCGAGTCCTACGTCAGGCTC47710.1050718589328258No Hit
CTGCAGCTCGGGTCATGATCTCAGGGTCTGGAGATGGAGTCCCACCAGAG47700.10504983590643033No Hit
ACCTGAGCCAAAGGCAGTTGCTCAACCAACTGAGCCACCCAGGTGCCCCT47350.10427902998258859No Hit
ATGGAGAGGTCAGAGGAAGCGCAGACTCCCCGCCGAACAGGGAGCCTGAC46750.10295764839885992No Hit
GCTCCCTGCTGAGCAAGGATCCTCTGGTGGGACTCCATCTCCAGACCCTG46700.10284753326688252No Hit
CAAGAACGTGTCAGGGAAAACTGGCTGTGAGCTTTTATGTCAGTCAAGAA46000.10130592141919904No Hit
GCCTGACGTAGGACTCGGTCCCAGGACTCCAGGATCATGACCTGAGCCAA45950.10119580628722166No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC45890.10106366812884879No Hit
CCCAGGTGCCCCTCATTTCTGCTTTCTTGACTGACATAAAAGCTCACAGC45840.1009535529968714No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT45780.10082141483849855No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)