FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D7.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D7.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4359939
Sequences flagged as poor quality0
Sequence length151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAGGGCAGCGAGGA324350.7439324265775278No Hit
GTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTC280400.6431282639504818No Hit
GGAATAAGCAGGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGG258500.5928982033922952No Hit
ATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAA174800.40092304043703364No Hit
GTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGC155880.3575279378908741No Hit
GTGTATGCACATCTCTGATAGCAGGGACAGGTTTGTGCATGTCTCAGGCA149050.3418625811049191No Hit
GTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTA142690.32727522105240464No Hit
GGTCAGAAGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCAC136450.3129630942084281No Hit
GCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGC136400.31284841370487065No Hit
CTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATG128280.294224299927132No Hit
GGACAGATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCAC123770.28388011850624517No Hit
CACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTGCCTGA112290.25754947488944224No Hit
GATGTGTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCACGTCTC105500.24197586250633324No Hit
GTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGT104460.23959050803233714No Hit
GGCTGGCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAG102580.23527852109857503No Hit
CTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCACGGCAACCT98580.22610408081397468No Hit
CATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTAGACT97360.22330587652717157No Hit
CTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGA97110.2227324740093841No Hit
GGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTG96100.2204159278375225No Hit
GCACACACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTA95960.22009482242756148No Hit
GTCTCAGGCAGTGAGGAGAGGTGTATGCAGGTCTTTGATAGCAGGGACAG94410.21653972681727884No Hit
GGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGC94160.21596632429949134No Hit
GTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCAGCAG93410.21424611674612878No Hit
CCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGTCAGGGTTCA92230.2115396568621717No Hit
GGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTGGATATGACCATT89720.20578269558358503No Hit
CTTTATTTATTTGCTTATTTATTTTTTGAGGTAGAGTCTCACTCTGTTAC88910.20392487142595345No Hit
TCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCCAGGCCA83340.1911494633296475No Hit
CCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAACC82640.18954393627984245No Hit
GTCTCACTCTGTTACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC81980.1880301536328834No Hit
GGTGTATGCAGGTCTTTGATAGCAGGGACAGGTGTGTGCAGGTCTCAGGC81920.18789253702861441No Hit
GTCTAGGACCTACCCCTTATCTCTCTCTCTTGCTCCCACTGTCACTATGT80500.18463561072758128No Hit
GCACACACCTGTCCCTGCTATCAAAGACCTGCATACACCTCTCCTCACTG79530.1824108089585657No Hit
GGTGTGTGCTGGTCTCAGGCAGGGGGACAGGTGCGTGCAGGTCTCAGGCA77540.17784652491697706No Hit
CCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGT76750.17603457296076847No Hit
CCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCAT76240.17486483182448193No Hit
ACCCTGTCTCTACTAAAAATACAAAAATTAGGCGGGTGTGGTGGTCCACG75700.1736262823860609No Hit
ACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTCTCTATCA75100.17225011634337087No Hit
TCTCAGGAAGAGGGACAAGTGTATGCACATCTCTGATAGCAGGGACAGGT74130.17002531457435527No Hit
CATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAGTGCTCTCTGC74100.16995650627222078No Hit
CTTCCTGCCCTTGCCCTGGGTCAGGGTTCATCCTCCCAGCCCGATGTGCA73980.16968127306368277No Hit
GCATACACCTCTCCTCACTGCCTGAGACATGCACAAACCTGTCCCTGCTA73670.16897025394162624No Hit
CTCCTATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAG73460.16848859582668474No Hit
GAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATCTC73410.16837391532312723No Hit
GGATAGGAGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGA72310.16585094424486213No Hit
GAGCAGAGAGCACTCCATTCTGGATATGACCATTTTCTGCACAGCCACCA70410.161493085109677No Hit
CCATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAG69790.16007104686556395No Hit
GATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATA69370.1591077306356809No Hit
GGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAG69270.15887836962856589No Hit
AGCCTAGAGTGCTTCTCTATCAGGCACATGGTGGCTGTGCAGAAAATGGT69010.15828203101006688No Hit
CACCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAA68750.15768569239156788No Hit
GGGTAGGGTCCGCCATCAGGAGCTTAGTGGAGAGACCATTGTGCAACATG68630.15741045918302984No Hit
ATCTTGGCTCACGGCAACCTCTGCCTCCCAGGTTGAAGTGATTCTCCTCC68100.1561948458453203No Hit
AAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCT66540.1526168141343262No Hit
TTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTAT65410.15002503475392662No Hit
GCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTG65390.1499791625525036No Hit
AGCCAGTACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGG64870.14878648531550556No Hit
GGGTAACAGAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAA64760.14853418820767905No Hit
ACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATG63730.14617176983439448No Hit
GGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAGAGAAGCACT63530.14571304782016445No Hit
GTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCAC62950.1443827539788974No Hit
CGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGGAGAATCACTTCAA62010.14222676051201635No Hit
ATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCTTC61950.14208914390774732No Hit
GCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGC59930.13745605156402418No Hit
GTTTGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTACTAAAAAT59830.13722669055690917No Hit
GAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCC59710.13695145734837116No Hit
CTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCACTCCATTCTG59560.13660741583769864No Hit
CCATGTGCCTGATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCG59320.13605694942062263No Hit
ATCCAGAATGGAGTGCTCTCTGCTCTCACCAAGACCTGGCCTCTGCAGCA59000.1353229941978546No Hit
GCCTGAGACATGCACAAACCTGTCCCTGCTATCAGAGATGTGCATACACT58580.13435967796797155No Hit
CCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGCAC58060.13316700073097354No Hit
CCCCTCACCCCCTCTGCTGCAGAGGCCAGGTCTTGGTGAGAGCAGAGAGC56990.13071283795484295No Hit
GATAAGGGGTAGGTCCTAGACTCTTTATTTATTTGCTTATTTATTTTTTG56690.1300247549334979No Hit
GAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAGGCCGCAGCAGGG55670.12768527266092483No Hit
TCTCTGATAGCAGGGACAGGTTTGTGCATGTCTCAGGCAGTGAGGAGAGG55040.12624029831610029No Hit
ACATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCC54910.12594212900685078No Hit
GAGAACAGGTGTGTGCACGTCTCAGGAAGAGGGACAAGTGTATGCACATC54190.12429072975562272No Hit
CTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGGG54180.12426779365491124No Hit
GGGCAAGGGCAGGAAGAGGGGAGCTGAGTTCATAGGATGTACGACTCAAG53070.12172188647593463No Hit
GCTCCAGGCCAGCAGGGCAGCGAGGAGGCTGGCACTGCACATCGGGCTGG52870.12126316446170463No Hit
GGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAGGAAGAGGGGAGCT51500.11812091866422902No Hit
GGTTTGTGCATGTCTCAGGCAGTGAGGAGAGGTGTATGCAGGTCTTTGAT51450.11800623816067152No Hit
AGCTCATCCTGTGATGGAGCAGGCTAGGATGGGTGGGAGGCACCAGCTCC51340.11775394105284501No Hit
AATTAGGCGGGTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAG51260.11757045224715301No Hit
ATCCTCCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCC50220.11518509777315691No Hit
GTGTGGTGGTCCACGCCTGTAGTCTCAACTACTTGGGAAGCTGAGGGAGG50080.1148639923631959No Hit
GGACAGGTGTGTGCTGGTCTCAGGCAGGGGGACAGGTGCGTGCAGGTCTC50060.11481812016177291No Hit
CTATCAGGCACATGGTGGCTGTGCAGAAAATGGTCATATCCAGAATGGAG50020.1147263757589269No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC49620.11380893173046688No Hit
CCCCTGCTGCGGCCTTGAGTCGTACATCCTATGAACTCAGCTCCCCTCTT49400.11330433751481385No Hit
AGCGAAAGCAGGGGAGGATGGACCCCTCACCCCCTCTGCTGCAGAGGCCA49210.11286855160129534No Hit
CCTGTCCCCAATGCCTGAGACCTGCACACACCTGTCCCTGCTATCAAAGA48090.11029970832160726No Hit
GAGTGAGACTCTACCTCAAAAAATAAATAAGCAAATAAATAAAGAGTCTA48060.11023090001947276No Hit
ATAGAGAAGCACTCTAGGCTCCTATCCTCCCCTGCTGCGGCCTTGAGTCG47930.10993273071022323No Hit
GCACTGCACATCGGGCTGGGAGGATGAACCCTGACCCAGGGCAAGGGCAG47420.1087629895739367No Hit
GGATGTACGACTCAAGGCCGCAGCAGGGGAGGATAGGAGCCTAGAGTGCT46260.1061024018914026No Hit
GTCGTACATCCTATGAACTCAGCTCCCCTCTTCCTGCCCTTGCCCTGGGT46120.1057812964814416No Hit
GGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGCGAAACCCTGTCTCTA45860.10518495786294257No Hit
GGCCAACATGGCGAAACCCTGTCTCTACTAAAAATACAAAAATTAGGCGG45720.10486385245298156No Hit
ATCAAAGACCTGCATACACCTCTCCTCACTGCCTGAGACATGCACAAACC45220.10371704741740653No Hit
ATCACATAGTGACAGTGGGAGCAAGAGAGAGAGATAAGGGGTAGGTCCTA45010.10323538930246501No Hit
AACCTGAGGTCAGGAGTTTGAGACCAGCCTGGCCAACATGGCGAAACCCT44770.10268492288538898No Hit
GTGCAGGTCTCTGCTATCAGAGACCTGCACACACCTGTCCCCAATGCCTG44590.10227207307258199No Hit
CTCCATTCTGGATATGACCATTTTCTGCACAGCCACCATGTGCCTGATAG44370.10176747885692897No Hit
GTGCATGCAGGTCTCAGGCACTGAGAACAGGTGTGTGCACGTCTCAGGAA43770.10039131281423892No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)