FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D6a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D6a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences991098
Sequences flagged as poor quality0
Sequence length151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTG55050.5554445675402433No Hit
GTCCAGGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAG46960.47381792718782606No Hit
GTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGG41260.41630595561690165No Hit
GGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGCAGCGGGGCTGGGCAG34260.345677218599977No Hit
GAATTGGATGGGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTA33570.33871524309402296No Hit
GTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGCCCCAGGACTT33110.33407392609005365No Hit
GCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTG32010.3229751245588226No Hit
CTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCA27090.27333321225549845No Hit
CAGTAGGAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGG26670.26909548803448297No Hit
GGGCTGGGCAGCGAGACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAG24500.2472005795592363No Hit
ACTGAATACAGACTGAACTATGTATTGCTGTGCATGATGGAGCGCACTGG24090.24306375353395931No Hit
GTATTCAGTAAATGCCATCACCATCTTCCCAGTGGCTGAGGCCAAAACCT23750.23963321487885156No Hit
GAACTATGTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCA23370.2357990834407899No Hit
GCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCA23360.23569818524505146No Hit
TCCTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTC21360.2155185460973587No Hit
GGTCAGAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACT21190.21380327676980482No Hit
GGCAAGCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTC21130.21319788759537403No Hit
TTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGG20130.20310806802152762No Hit
ACATAGTTCAGTCTGTATTCAGTAAATGCCATCACCATCTTCCCAGTGGC19940.2011910023024968No Hit
CCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGGAGAAAG19000.1917065719030812No Hit
ATGCTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTT18350.18514818918008108No Hit
CACTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGT18190.18353381804826566No Hit
GTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGT17520.17677363893378858No Hit
GAGGAAGGGAGTGGAGAAAGAAGAGAGGAATCAAAGGCCACGGGGGTTTT17230.17384759125737315No Hit
GCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAATAATTAGTGT15970.1611344185943267No Hit
GGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTGGCCTC15960.16103352039858823No Hit
ATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTG15320.15457603587132654No Hit
GTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGGTTGCATGGCA14900.15033831165031106No Hit
CAGCGGGGCTGGGCAGCGAGACTTGCACCGGAGCCTTTGCCACAGTCCAG14860.1499347188673572No Hit
GGCGTAGTAGGTATTTTGGAAGGTGAACAGATGGGGCTTACTGAAGAGGG14770.14902663510571104No Hit
GAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTTTG14690.14821944953980332No Hit
GCACTAGTGCGTGGGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGCAG14260.14388082712304937No Hit
GAGCACTAGTGCGTGGGCTGGGACTGGGGGGCCGAGGCCGAGAAACACGC14220.14347723434009552No Hit
GAAAGGGCCAGTGCCAGGGAGAACTGAAAGGAGGGAACAGACACTAGAGC14140.14267004877418782No Hit
GGACTGGGGGGCCGAGGCCGAGAAACACGCAGCGGGGCTGGGCAGCGAGA14070.14196376140401856No Hit
GATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGA13940.14065208485941855No Hit
ACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTT13940.14065208485941855No Hit
TAATTAGTGTCAGCCATTCTTCCTGTCCTCTTGCAGGTTAAAACCCCCGT13570.13691885161709538No Hit
GGGGAAGGAATTGGATGGGCGTAGTAGGTATTTTGGAAGGTGAACAGATG13340.13459819311511073No Hit
GGATTTGAGGATGAGCCCTAGGTTTTGGCCTCAGCCACTGGGAAGATGGT13300.13419460033215685No Hit
GTATTGCTGTGCATGATGGAGCGCACTGGCAAGCACTACAGCATTTTGAA12980.130965858068526No Hit
AGCGAAAGCAGGGGAAGGAATTGGATGGGCGTAGTAGGTATTTTGGAAGG12950.13066316348131063No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT12800.12914969054523368No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA12790.1290487923494952No Hit
GACTTGCACCGGAGCCTTTGCCACAGTCCAGGTCAGAAAGGGCCAGTGCC12700.12814070858784904No Hit
GGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCT12640.12753531941341825No Hit
ACCTAGGGCTCATCCTCAAATCCTCTGCTGCCATGCAACCCTCTTCAGTA12580.12692993023898747No Hit
ATCATGCACAGCAATACATAGTTCAGTCTGTATTCAGTAAATGCCATCAC12480.12592094828160283No Hit
GTTTTGGCCTCAGCCACTGGGAAGATGGTGATGGCATTTACTGAATACAG12330.12440747534552586No Hit
CTAGAGCCCCAGGACTTATGGGGAAGCAGACAGTAGGAGGAAGGGAGTGG12180.12289400240944892No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG12110.12218771503927967No Hit
GCACTACAGCATTTTGAATAATTAGTGTCAGCCATTCTTCCTGTCCTCTT12090.12198591864780274No Hit
AAAGCAGGCTGGTGCGGCAGGAGCACTAGTGCGTGGGCTGGGACTGGGGG11820.11926166736286421No Hit
CTCCTACTGTCTGCTTCCCCATAAGTCCTGGGGCTCTAGTGTCTGTTCCC11680.11784909262252573No Hit
GTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAGTCTGT11410.1151248413375872No Hit
CTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGATGAGCCCTAGGTTT11370.11472124855463335No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC11350.11451945216315641No Hit
GTGAACAGATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTG11130.11229969185691022No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA11010.11108891350804864No Hit
ATATACAACAGGATGGGAACAGTGACCACAGAAGCTGCTTTTGGTCTAGT10900.10997903335492555No Hit
GGTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAA10810.10907094959327938No Hit
GTTTTAACCTGCAAGAGGACAGGAAGAATGGCTGACACTAATTATTCAAA10660.10755747665720242No Hit
GAAGAGAGGAATCAAAGGCCACGGGGGTTTTAACCTGCAAGAGGACAGGA10380.10473232717652543No Hit
GAATACAGACTGAACTATGTATTGCTGTGCATGATGGAGCGCACTGGCAA10330.10422783619783312No Hit
CTGTAGTGCTTGCCAGTGCGCTCCATCATGCACAGCAATACATAGTTCAG10190.10281526145749462No Hit
CAGATGGGGCTTACTGAAGAGGGTTGCATGGCAGCAGAGGATTTGAGGAT10180.10271436326175615No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)