FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D4.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D4.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11994422
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATTATGGTGCCTCCATACATGGAAATTGGCAGACCTTTAGAAAAATCC537820.4483917607701313No Hit
CCATAATACCCTCCCATCCTCAGCTTGCTGATGTTCCAGGTGGACCTCTG425200.35449811587419555No Hit
GATTAGGAGAGGCCACCATTGGCTGTCCAACTCTCTTCTTCGGTCTGGTT363590.303132572790919No Hit
CCATAAGGGGTGGTACCGAATCAACAAGATATGCAGTTCAACTTATCAAT321930.26839976115564385No Hit
CATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGA286990.23926955379759024No Hit
GAGTAGCAGTGACGGGTATCTCCTATCATTAGAGGAATTCACATCCATAG274920.2292065428413307No Hit
ATCACAGACTGTGACCAACCTGATAAGCCTTCTGACATAGCGTTGACTCC274410.2287813451952916No Hit
CAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGGCTTATCAG272420.2271222406548644No Hit
CTGCTACTCCATTCCCAAGAAGAAAAGACAAGTACTGCTACTTCCAAAAC258800.21576696234299575No Hit
GACTTAAAGCTTGTTGATAAGGAATTAGAATTCGTTTCACCTTCAAGTCG253860.21164838122253826No Hit
CTCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCT245100.20434498636116022No Hit
GTCTGTGATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCA243670.20315276551050146No Hit
ACCCAGAGGTGGTGGTACAGGTGGGAATAGTGACTCGGATAACTTGGACA236420.1971082891697491No Hit
CCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTTATCCACA207560.1730471047291816No Hit
TCCTTGGACAAGCGCCTCAAACTCATCCACCTCTGCCCCACCAACTTTGG199500.16632731448001414No Hit
GTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACG195540.16302577981665145No Hit
CCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCAGA186230.1552638384742508No Hit
ACATATATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAG184140.15352136184636492No Hit
CTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAA180680.1506366876202955No Hit
GGATATATGTTGTTCTGGTAATATTAAATGTGCAGTGGGAGGGGTCTCCT180650.1506116759940579No Hit
ATACAAGACGTTACGGGTGCGCATATTGATGTGGATAAACAAAAAGACAA172790.14405862991980772No Hit
CCCAAAGGAAATGCATTTTTACTGGAAGCTGCTGTGGTGCCAACTCCAGA167980.1400484325130465No Hit
ACCTATGGCAAGCAGTTTTCTGGATTTTTCTAAAGGTCTGCCAATTTCCA164370.1370387001557891No Hit
GAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTTCACTATGGATGTGA162050.13510446772674833No Hit
ATGTTGCACCCTCCCCTTCCCATTATCCTGGAAACCACGGAAGCTGGAAC156420.13041061920282612No Hit
CTGCTACTCTCACTGTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACT152570.12720079383566793No Hit
GACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTAAATTT148780.12404099172098498No Hit
GCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAGCCAAGAGTTA148480.123790875458609No Hit
CCTCTGGGTTCTCCATTTATTTTATTTTTATCAGCTTTTTGCTGTGCTAA146660.12227350346686151No Hit
CCCGTAACGTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTAT146520.12215678254441939No Hit
CCCCTGTGGGGACCAGTTTTGTTGCTCCTGTTGGACACAGTGGGATCTGG143050.11926377110960411No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG141970.11836335256505065No Hit
GTGCTAGGAGTTGTCACCACATTGGCCCTTTTTTTCCCAAACACATTTGT139570.11636242246604296No Hit
TCCTAGCACCAATCGGAAAAATAAGAAGAACAAAACAAAGGAGACCCCTC137640.11475334117809095No Hit
GTTGCAGTCTGTGCTGTCCAAGTTATCCGAGTCACTATTCCCACCTGTAC136470.11377788775482471No Hit
ATCCTGGAAACCACGGAAGCTGGAACGGACAACTTCTTTGACCTTCGTAC136260.11360280637116153No Hit
AACTTGGACAGCACAGACTGCAACAGTGAGAGTAGCAGTGGTGGTAAAAG133480.11128506233981095No Hit
TCCTTTGGGTGCTCCAGCTCTCGTAACATCACAGGCGACAACATTATCAA133420.11123503908733576No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA132200.1102178996203402No Hit
CATTGGAACAGAGAGGCTAGCCCGGATTCGGCAAGGAGGATCTGTCGCAC132160.11018455078535673No Hit
ATCCTTAGCACAGCAAAAAGCTGATAAAAATAAAATAAATGGAGAACCCA132130.11015953915911912No Hit
CATTAGAGGAATTCACATCCATAGTGAAATTTAACTCTTGGCTTTTACCA131670.10977602755680932No Hit
ATTCCCACCTGTACCACCACCTCTGGGTTCTCCATTTATTTTATTTTTAT131390.10954258571192509No Hit
GTGCAACATCACTGCTATACAAGACGTTACGGGTGCGCATATTGATGTGG130640.1089172950559852No Hit
GTCTTGTATAGCAGTGATGTTGCACCCTCCCCTTCCCATTATCCTGGAAA128960.10751664398667982No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT127250.10609098129113682No Hit
GCCATAGGTTGATGAAAAATGTTAGAGGGGGCTACCATTCCAGAATCATC126810.10572414410631875No Hit
GGTGGGAATAGTGACTCGGATAACTTGGACAGCACAGACTGCAACAGTGA126580.10553238830516384No Hit
GTTGGACACAGTGGGATCTGGTCATTTGGAGTCAACGCTATGTCAGAAGG124900.10413173723585846No Hit
GTCCAAGGATGGGGGGAAAAATGTTGCCTGTTGAAACTTGGAGTCCCAAC124860.104098388400875No Hit
ATAGGGAACCATCCAATGCATCAACAATTATCAGACCCAAGCACATTCTC124310.10363984191985243No Hit
GTTCCAAGCAGGATCTGGGTTGTGAAGTCCATTAAACAGAGGTCCACCTG122190.10187235366572896No Hit
CCATTAAACAGAGGTCCACCTGGAACATCAGCAAGCTGAGGATGGGAGGG121880.10161390019460713No Hit
GTACCACCCCTTATGGTGATCATTCTCTCGCCGTTCTTGTCTTTTTGTTT120630.10057174910137395No Hit
TTTTCCAAGTGTCCAAGTACAGAGTTACCCAGGAACGCTGAATTTGCTGG120040.10007985378536789No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)