FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D3a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D3a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1453578
Sequences flagged as poor quality0
Sequence length151
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGT92410.6357415976301237No Hit
ATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAA88890.6115254908921296No Hit
GATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATA78140.5375700512803578No Hit
GTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTA76950.5293833561047292No Hit
TTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGAC75140.5169313239468402No Hit
GTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGC70190.482877423846536No Hit
CCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGA60570.4166959048637225No Hit
GCCTATCTGCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTG51790.356293229534294No Hit
GTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTGTC46030.31666687305394No Hit
CTTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAA44720.30765462878497063No Hit
TCCCTGTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTG44520.3062787136294028No Hit
TTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGGGTAATTCAGAACAAA44280.3046276154427213No Hit
GTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTCATACGTGAGAG43990.30263253846714794No Hit
ATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAGACATGAT42260.2907308723714861No Hit
GTTGATTACCTGTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAA37890.2606671262223286No Hit
GCGTACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTAC37410.2573649298489658No Hit
CATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAG35920.24711436193998534No Hit
ATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCT34300.23596944917988577No Hit
GTCTTAGAAGACAGAGATCATGTCTCTCTCCAGAGCACAAAGGCAGGTTT32770.2254436982397917No Hit
GTTAGAGCCTCTCACGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGC31540.21698182003304947No Hit
ACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGG31100.21395480669080025No Hit
ATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAGAGCCTCT29470.20274109817292224No Hit
AAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAG29180.2007460211973489No Hit
GTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCACGTA28570.19654947997286695No Hit
GACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATC28350.19503597330174233No Hit
CGTATGAGGGACCAAAAGAGTTAAAACAGCTAAAGCATTAAGACAGGATG27240.18739964418834076No Hit
GCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGCAAGCAAACCTG26930.1852669756972106No Hit
TATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGT26780.18423503933053473No Hit
CTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAGAG26720.18382226478386438No Hit
ATCTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAGAG25940.17845619567714976No Hit
TTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCCCTC25820.17763064658380906No Hit
GCATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTG25360.174466041726003No Hit
GTACCGACCCTGTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATT25200.17336530960154875No Hit
ATATATCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAG25100.1726773520237648No Hit
GGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAATCACCT24610.16930635989262358No Hit
CATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTCTGAA24380.16772405746372057No Hit
GTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAATC22360.15382731439248531No Hit
CTGTTATTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTAC21510.14797967498132195No Hit
TGCAGGGACAGGGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCA21470.14770449195020838No Hit
GTAGAAACAAGGGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACAT21430.1474293089190948No Hit
GACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTT20370.14013695859458522No Hit
TACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCGTACA20350.13999936707902844No Hit
GGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCA19990.1375227197990063No Hit
TATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTT19960.13731633252567113No Hit
ATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAAAA19800.13621560040121686No Hit
CCATAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATC19730.13573403009676813No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC19700.13552764282343294No Hit
GAGCAAAAGCAGGGTGACAAAAACATAATGGACTCCAACACCATGTCAAG19690.13545884706565456No Hit
TCTCCATATATATTGATCTGCCTATCTGCGTACATACATCGATAGTATTC19270.13256942523896206No Hit
CTACTACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTT19200.1320878549345133No Hit
CCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAATAACAGCAC19110.1314686931145078No Hit
TAGTAATAACAGCACGTATTTATTAAGGGACTACTACATTCCACATCCTG19050.13105591856783744No Hit
GCTTAGCACAGGCAGACCCAGTCTTTCCTAGCAGCCTCTGCGCAAACCTA19000.13071193977894546No Hit
TTACTATGGTGCAAAAATCATCTTGCTGGGTGGAATACAGGTACTAGTGC18650.12830408825670173No Hit
GAATGTAGTAGTCCCTTAATAAATACGTGCTGTTATTACTATGGTGCAAA18510.12734094764780424No Hit
GTCCCTGCATGTCCTTCCAGCACAGGCATTGGTGAAATTTTGTTCTGAAT18380.12644660279668513No Hit
GAGCAAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGA18260.12562105370334445No Hit
ATGTTACGTAGACAGATATATCTCCATATATATTGATCTGCCTATCTGCG18230.12541466643000926No Hit
TACATACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTG18000.12383236400110623No Hit
AACCTAACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGG17900.1231444064233223No Hit
TACATCGATAGTATTCTGAAAGCAATGGGGATCTGTTGATTACCTGTTAG17710.12183728702553287No Hit
GGGTAATTCAGAACAAAATTTCACCAATGCCTGTGCTGGAAGGACATGCA17350.11936063974551073No Hit
GGCACGGGTGATGCCACTCGCACACTGAGTTTGTGCCAAAGCCGAAGAGC17150.11798472458994289No Hit
TATTTATTAAGGGACTACTACATTCCACATCCTGTCTTAATGCTTTAGCT17030.11715917549660218No Hit
CACTGAGTTTGTGCCAAAGCCGAAGAGCTTAGCACAGGCAGACCCAGTCT16790.11550807730992077No Hit
CATTAAGACAGGATGTGGAATGTAGTAGTCCCTTAATAAATACGTGCTGT16320.11227467669433631No Hit
GTGGAATACAGGTACTAGTGCCTCCCTGTACCGACCCTGTCCCTGCATGT16040.11034839547654134No Hit
AACCATGACTTGCCTTAGAGGCAGACAGGATCTTTATGATACTGGACAGC15990.11000441668764936No Hit
GCTCTGGAGAGAGACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAAT15890.10931645910986545No Hit
GACATGATCTCTGTCTTCTAAGACTGTTTGCTAAAAATCACCTTGAAGGG15790.10862850153208153No Hit
CACTAGTACCTGTATTCCACCCAGCAAGATGATTTTTGCACCATAGTAAT15750.10835331850096795No Hit
GGATCTTTATGATACTGGACAGCAAGCAAACCTGCCTTTGTGCTCTGGAG15390.10587667122094584No Hit
GGTCGGTACAGGGAGGCACTAGTACCTGTATTCCACCCAGCAAGATGATT15270.10505112212760512No Hit
GGCTTGAATTGGTTGGTTTGAACTCGTCTCAAAATACATGCAGAGAACCC15210.10463834758093478No Hit
ACATTCCACATCCTGTCTTAATGCTTTAGCTGTTTTAACTCTTTTGGTCC14910.102574474847583No Hit
GTTTTAACTCTTTTGGTCCCTCATACGTGAGAGGCTCTAACAGGTAATCA14890.1024368833320262No Hit
GTATTATATGGCATGAATATGGCACACTTTGTTTATCCATTCTCTAGTTG14820.10195531302757746No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)