FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2839251
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCG148420.5227434981972358No Hit
ATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACAGTAC136830.4819228733211682No Hit
ATGTAGGACCATGAGCTTGCTGTGGAGAGTGATTCACACTCTGGATTTCC131930.46466480068158816No Hit
CTGTAGACACAGTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAAC112660.3967947884847095No Hit
GTATAAGACCTTGCTTCTGGGTTGAACTAATCAGAGGGCGACCCAAAGAG108440.3819317136808264No Hit
GCTATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATC95640.33684940147947473No Hit
GTCCTACATTGTGGAAACACCTAGTTCAGACAATGGAACGTGTTACCCAG82380.29014694368338695No Hit
CATTATGTATAGGTTATCATGCGAACAATTCAACAGACACTGTAGACACA80850.2847581985530691No Hit
ACTCAGCACCGTCTGGCCAAGACCAACCCACAGTGTCACTGTTTACACCA74560.2626044685728736No Hit
GTCCAGTATCGTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAA70920.24978418604061423No Hit
GGATACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAA62080.21864921417655572No Hit
GTACTAGAAAAGAATGTAACAGTAACACACTCTGTTAACCTTCTAGAAGA61010.214880614640974No Hit
ATTCTACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCC60750.21396488017438403No Hit
TACTAGTAGTTCTGCTATATACATTTGCAACCGCAAATGCAGACACATTA58160.20484275606489177No Hit
TTCTAGTACTGTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTA57200.2014615826497904No Hit
GCTATATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGA56080.1975168803321721No Hit
GGTTAACAGAGTGTGTTACTGTTACATTCTTTTCTAGTACTGTGTCTACA54060.1904023279378963No Hit
GACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAAATGAGTGGTC51010.17966005823366799No Hit
GACTGGGACAGACAATAACTTCTCAATAAAGCAAGATATCGTAGGAATAA50550.1780399126389319No Hit
GTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGGAGACT48020.16912911186788346No Hit
ATGTTACATTTACCCAAATGCAATGGGGCTACCCCTCTTAGTTTGCATAG47210.1662762467988917No Hit
GCTGTGAACTGTGTATTCATCTTTTCAATAACAGAATTTACTTTGTTAGT47100.16588882067841132No Hit
GTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAACCGTTTCTTGA46370.16331772006067796No Hit
GTCTAATGGAGCAAATGGAGTAAAAGGGTTTTCATTCAAATACGGCAATG46270.16296551449660493No Hit
GTGTCTACAGTGTCTGTTGAATTGTTCGCATGATAACCTATACATAATGT46220.1627894117145684No Hit
CATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGACAG45720.16102838389420307No Hit
GGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTGGATTG44770.157682431035509No Hit
ATCCTGACCACTCATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTA43930.1547239042972953No Hit
CTAGTAGAGCCGGGAGACAAAATAACATTCGAAGCAACTGGAAATCTAGT42220.14870118915164598No Hit
TGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAAATGGAG40860.14391119348025236No Hit
ATATACATCCGATCACAATTGGAAAATGTCCAAAATATGTAAAAAGCACA40700.14334766457773546No Hit
CCCCTATGTCTCCCCATATCCCTTGTGAGGTTAGGTTTGTATACCCACAA40360.14215016565988706No Hit
GATGTATATTCTGAAATGGGAGGCTGGTGTTTATAGCACCCTTGGGTGTT38950.13718406720645693No Hit
GTTATTGTCTGTCCCAGTCCATCCGTTCGGATCCCAAATCATCTCAAAAC38550.13577524495016466No Hit
TACATATGCAGTGGGATTTTCGGAGACAATCCACGCCCTAATGATAAGAC38420.1353173777168697No Hit
CATTTATTCCTACGATATCTTGCTTTATTGAGAAGTTATTGTCTGTCCCA37370.13161921929410256No Hit
ATATACATTTGCAACCGCAAATGCAGACACATTATGTATAGGTTATCATG37040.13045694093266147No Hit
CTGTTAACCTTCTAGAAGACAAGCATAACGGGAAACTATGCAAACTAAGA36960.130175176481403No Hit
CTCTTAGTTTGCATAGTTTCCCGTTATGCTTGTCTTCTAGAAGGTTAACA36010.12682922362270893No Hit
CCCTAATGATAAGACAGGCAGTTGTGGTCCAGTATCGTCTAATGGAGCAA35650.12556128359204594No Hit
ACACTGTAGAGACCCATTAGAGCACATCCAGAAACTGATTGCCCCCAGGG33600.1183410695285482No Hit
GTCCTGGACTGAGATCTGTCAGGTTTTGCCTGGGTCCCTTTGCACCACTG33330.11739011450555092No Hit
ATACAATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATA33150.11675614449021943No Hit
GTGTTTGGATAGGGAGAACTAAAAGCATTAGTTCAAGAAACGGTTTTGAG32300.11376239719559841No Hit
AGGATATGCTGCTCCCGCTAGTCCAGATTGTGTTCTCTTTGGGTCGCCCT32210.11344541218793265No Hit
GGCCAAGACCAACCCACAGTGTCACTGTTTACACCACAAAAGGATATGCT32140.11319886829308153No Hit
GTGTCACTGTTTACACCACAAAAGGATATGCTGCTCCCGCTAGTCCAGAT31890.11231835438289886No Hit
TCTTTATCCCGTCCCTCTGGGACACACGGATGGCTCCACTGGCTTTTCTA30870.10872585762935366No Hit
CTCCAACACCATGTCAAGCTTTCAGGTAGACTGTTTCCTTTGGCATATCC30780.1084088726216879No Hit
GTTCACAGCAGTAGGTAAAGAGTTCAACCACCTGGAAAAAAGAATAGAGA30730.10823276983965137No Hit
GAATAAATGAGTGGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAA30460.1072818148166541No Hit
CTCCTTGGTTGACCTAGAAAAGCCAGTGGAGCCATCCGTGTGTCCCAGAG30090.10597865422958379No Hit
ATCCAGCCCTGTTAGTTCTGGATGCTGAACAAAACTCCCGCTATATCCTG29910.10534468421425228No Hit
ATACTGGACCACAACTGCCTGTCTTATCATTAGGGCGTGGATTGTCTCCG29480.10383020028873811No Hit
GGTCAGGATATAGCGGGAGTTTTGTTCAGCATCCAGAACTAACAGGGCTG28430.100132041865971No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)