FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D2a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D2a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences811215
Sequences flagged as poor quality0
Sequence length151
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CATATAGATAGATAGATCTTTACTCTGGACAGTTAGGTGGGAACAGTTTT17950.22127302872851218No Hit
ATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTCTT13800.17011519757400934No Hit
TGGTATTACTGTCCAACTGAAAGGAAACATACTCTTAGAAGTGTATTTTG12900.15902072816700874No Hit
GTGCAGGGGTTGATCCCCCGGACCCTGGGATCATGACCTGAGCCGAAAAG12460.15359676534580846No Hit
ACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTCCTGAGCC11940.1471866274662081No Hit
ATATTGTACCTTGTCATTTGCAAATAGTGACAGTTTTACTTTTTCCTACC11900.14669353993700807No Hit
CTATACAATCTGTACCCCGATGATTAAATGCAATTCTAGTTCTCGAACTC11770.145091005467108No Hit
ATATTAATATGGCTACTCAAAGCAACTCACTAGTCAGCTAGACATTAGAT11570.14262556782110783No Hit
CTATATTACCTGGCAAACAATATTTCGTGTTTCTAACTTATCTTTTCCTT11190.13794123629370758No Hit
GTCCTGAGGAGGGGAGGTGCTCAGGCCTTGTAGGTCTGGCCTTGAGGCCT10020.12351842606460679No Hit
ATTCCATAGTGTCTGAGAATGAGGGCCATACCCTGCTCCCTTAACCCAAA9650.11895736641950655No Hit
GTCAATGACAGTGGACTGTCCCTCGTGGAGCTTGGATGGACTGAGCATTC9590.1182177351257065No Hit
TATAAAAACCACTCAAGCTTTAGGTACTGCTATTAATGCTAGAAATAACT8760.10798616889480593No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG8700.10724653760100589No Hit
GTGTAAGGGCTCTTTATTTCTCATTGCGATGATGTACTCGTTAGGAATAA8700.10724653760100589No Hit
GGCTTAAAGATATGAAATGGAGTTATTTAATCCTCCCTCCTCAAAGTGCA8610.10613709066030583No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA8510.10490437183730576No Hit
GTATAGGAATAAAGCACTCTCATTAATCAAATATTTTATCTTTTGCTTTC8440.10404146866120573No Hit
GTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGGAAA8430.10391819677890571No Hit
AATGTATACAATGGACAGAATAGTTAATGTCAAAGTATTCTCAGGAAAGG8380.10330183736740568No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT8260.10182257477980561No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)