FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D10a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_D10a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493022
Sequences flagged as poor quality0
Sequence length151
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCTAATAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTC53301.0810876593742267No Hit
CTATTAGAACTGGGGTTTTATTGACAGAAACTGGTAAATTAGAAGGGGCA43220.876634308408144No Hit
ACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGT33660.6827281541188832No Hit
ATATTAGGTAAGTACTTAACACAGTACAATCTAGGCTGGCTCATCGACTT28130.5705627740749906No Hit
TACTAGGACTTGGCAACTGGACCTAATAGCTAAGAGAAAGTGAGAAGGAA25870.5247230346718808No Hit
TCCTAAGAGTAATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCA23220.4709728977611547No Hit
ATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAG22130.4488643508808937No Hit
CTATTAGCACCATTGCCTATAAATGCTACTATATTAGGTAAGTACTTAAC18820.381727387418817No Hit
GTGTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAATGGTGCTAAT18630.3778736040176706No Hit
ATTTTATTTAGTGTCATGTTTTCAAGATTCATGCATGTTGTAGCATGTAT18480.37483114343781815No Hit
GTATTAAAGTACATGTAACAGAATTTGCCAGTGTAATCATCTTTAAGTGT18360.37239717497393626No Hit
ATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGAGTTTGTGAAAG17830.36164714759179106No Hit
CCCTTAGGCATGCCAGCCACTCTCATGGCCAAAAATACTATCACTGTCTG17580.35657637995870367No Hit
GTACAGTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGAGTAATCT17090.34663767539785245No Hit
GTGCTAATAGTGAGAGTTTGTGAAAGAGCAGTTACTAGGACTTGGCAACT16810.3409584156487946No Hit
GTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAG16080.32615177416017943No Hit
ATGTATTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTT15910.32270365216968006No Hit
GTTGTGGACCATCGCCATCATCCATCTGCAGAACTTTCTTGCTCTTCCAA15830.3210810065270921No Hit
AGATTGTACTGTGTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAA15690.3182413766525632No Hit
GCCTAAGGGAGTGGGAAGTACAAAGAGCAGGTTCTGGGGCAGATCAAAAT15640.3172272231259457No Hit
CTTCAAACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTTCAAAATTC15610.31661873100997523No Hit
ACTTAACACAGTACAATCTAGGCTGGCTCATCGACTTAATAACTCAACTT15520.31479325466206376No Hit
CTTACACAGAGGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCC14930.30282624304797756No Hit
GTGCAGCAGCTACTGAAAACAATATGGCAGTTCATCAAAAAATTAAAAAT13870.28132618828368716No Hit
GTTCTAATAGTTCTGATACATGCTACAACATGCATGAATCTTGAAAACAT13560.27503843641865877No Hit
GTTATGAGTACAGTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGA13480.2734157907760708No Hit
ATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGAGCCAGCCT13210.26793936173233646No Hit
TATGAGTACAGTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGAGT13140.266519546795072No Hit
GCCCTAGGAAGAGTAAGAAAGAAAGGGGGGTGCAAGGTGTTTGGCCTGAG12810.2598261335193967No Hit
GTCCTAGTAACTGCTCTTTCACAAACTCTCACTATTAGCACCATTGCCTA12780.2592176414034262No Hit
GACTTACACAGAGGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTT12100.24542515344142857No Hit
GGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAA12050.24441099991481108No Hit
GCTGTACACATTACCTAACAAGTCAGCAAAGATGAGGTCTAAAATGTGAA11490.2330524804166954No Hit
GTTCTGATACATGCTACAACATGCATGAATCTTGAAAACATGACACTAAA11470.23264681900604842No Hit
GTACTTAACACAGTACAATCTAGGCTGGCTCATCGACTTAATAACTCAAC11430.2318354961847544No Hit
GTACTGAGTCCTTTGAGTCCCACTGGCAAATCCTAAGAGTAATCTTGGAG11410.23142983477410745No Hit
GTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGA10850.22007131527599175No Hit
CTACTATATTAGGTAAGTACTTAACACAGTACAATCTAGGCTGGCTCATC10720.21743451610678632No Hit
GTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGG10520.21337790200031642No Hit
CATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAATT10400.21094393353643445No Hit
CAATATGGCAGTTCATCAAAAAATTAAAAATTAAAAACGGATTACCGTAT10240.20769864225125856No Hit
CTGCTGCACCATGCCGCATTCCCAACAGCAGAATTTTAAGTGTTCTAGTT10020.20323636673414167No Hit
GTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAG9840.19958541403831878No Hit
GTACTGTGTTAAGTACTTACCTAATATAGTAGCATTTATAGGCAATGGTG9800.19877409121702483No Hit
TTTATAGGCAATGGTGCTAATAGTGAGAGTTTGTGAAAGAGCAGTTACTA9780.1983684298063778No Hit
GTTCATGGCATTGTTGTGGACCATCGCCATCATCCATCTGCAGAACTTTC9740.19755710698508383No Hit
AAATAAAATAAAGCAGACACAAAAGGACAAATAATACATAATTCCACTTA9670.19613729204781935No Hit
GGAGTATAGTGTGTGTCTACTTCCAAAGTCAAGTCTGAAACTAAAAATCA9670.19613729204781935No Hit
GTCCACAACAATGCCATGAACTGTACACTTAAAGATGATTACACTGGCAA9620.1951231385212019No Hit
GTACAATCTAGGCTGGCTCATCGACTTAATAACTCAACTTCTTTTCCACT9570.19410898499458443No Hit
ATCCTGATGAGTGTGTAGTTTGCTCTACTGAGGCTTTGATTCACATTTTA9340.18944387877214405No Hit
AGCTAAGAGAAAGTGAGAAGGAACTAAAAATGTTTTGAGGTTTCTAGCTA9300.18863255595085007No Hit
GACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATACA9270.18802406383487957No Hit
GTATAGAGTTTGGAAGAGCAAGAAAGTTCTGCAGATGGATGATGGCGATG9250.18761840242423258No Hit
ATCCAGGCATCCCTGGATTTTGTTTTTAGAATATGGGAAGATCTTGACTA9190.1864014181922916No Hit
GATGTGAGATGACCAAGATGTAGACCAGAATGAAGATGAAGCAGTGAGAA9140.18538726466567415No Hit
CAATGGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTG9000.18254763479114522No Hit
CATCAGGATGGCTACTAACAAAAACAAACAAACAAACAAAATAACAGTCC8900.1805193277379103No Hit
TAGTGAGAGTTTGTGAAAGAGCAGTTACTAGGACTTGGCAACTGGACCTA8830.17909951280064582No Hit
AGATGGGACCCTGCAAAAGAAGACGATGAAGAGATCTCTGGGAAATGAAT8780.17808535927402835No Hit
AAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGA8750.17747686715805785No Hit
GTCTTTTATCTTGAAAACCAAAGAATCTGCTTTGTCCTGTCCTAGTATCC8600.17443440657820544No Hit
AGCCTAGATTGTACTGTGTTAAGTACTTACCTAATATAGTAGCATTTATA8570.17382591446223494No Hit
TAGTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAG8560.17362308375691146No Hit
GTAATAGTCCATTGTGTGTCTTTATCAACATTTTGTTCACGTGTTCACCT8510.17260893023029397No Hit
ATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTAT8330.1689579775344711No Hit
ATTTAGTGTCATGTTTTCAAGATTCATGCATGTTGTAGCATGTATCAGAA8190.16611834765994216No Hit
ATAATACATAATTCCACTTACAAGAGATGCATAAAGAAACTGGACTGGAT8130.16490136342800119No Hit
TACTATCACTGTCTGAGTTGCTTTCAACAAAACTGAACTGTGCTTCGCTC8070.16368437919606021No Hit
GGTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAA8030.16287305637476623No Hit
GTGTACAGCTGATCAGTGTCCTCCTCCTTTTACTCTACCCGTCTGGTTTT7910.1604390879108843No Hit
GCTTTATTTTATTTAGTGTCATGTTTTCAAGATTCATGCATGTTGTAGCA7690.15597681239376743No Hit
GTACTGAAGGTACAGCCCTAGGAAGAGTAAGAAAGAAAGGGGGGTGCAAG7680.15577398168844392No Hit
GCCATATTGTTTTCAGTAGCTGCTGCACCATGCCGCATTCCCAACAGCAG7650.15516548957247345No Hit
CCTGATGAGTGTGTAGTTTGCTCTACTGAGGCTTTGATTCACATTTTAGA7580.15374567463520897No Hit
CTCATCAGGATGGCTACTAACAAAAACAAACAAACAAACAAAATAACAGT7580.15374567463520897No Hit
TATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAAGTAATAGTCCA7360.1492833991180921No Hit
GAGTAATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCATCACTT7320.14847207629679812No Hit
AAACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTTCAAAATTCACTG7300.14806641488615113No Hit
GTGATAGTATTTTTGGCCATGAGAGTGGCTGGCATGCCTAAGGGAGTGGG7280.14766075347550411No Hit
GCATAGTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGT7170.1454296157169457No Hit
CATTAAACACATCTTGCCAGCCCTCCTTCCCCTCAGTCCCTGGCAATCCA7170.1454296157169457No Hit
CTTGTAAGTGGAATTATGTATTATTTGTCCTTTTGTGTCTGCTTTATTTT7170.1454296157169457No Hit
GCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAAT7140.1448211236009752No Hit
CTATTAGAACTTCATTCCTTTTTAAGGTTAAGTAATAGTCCATTGTGTGT7140.1448211236009752No Hit
GTACTTACCTAATATAGTAGCATTTATAGGCAATGGTGCTAATAGTGAGA7130.14461829289565173No Hit
GTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGAT7080.14360413936903424No Hit
GTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGAGCC7070.14340130866371076No Hit
ACTCTATACACATTAAACACATCTTGCCAGCCCTCCTTCCCCTCAGTCCC6970.14137300161047578No Hit
GGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATAC6930.14056167878918183No Hit
GTATTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTTTT6920.14035884808385832No Hit
TTTCAGCATAGTAGATCTGATAGTATCAAAGTAGTATTCTAAGGTTACAT6900.13995318667321135No Hit
ACACAGTACAATCTAGGCTGGCTCATCGACTTAATAACTCAACTTCTTTT6830.13853337173594688No Hit
TAGTTACCCTAGCTAGAAACCTCAAAACATTTTTAGTTCCTTCTCACTTT6790.1377220489146529No Hit
ATGTTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTT6640.13467958833480048No Hit
TTACCTAACAAGTCAGCAAAGATGAGGTCTAAAATGTGAATCAAAGCCTC6540.13265128128156553No Hit
GAGTAAAAGGAGGAGGACACTGATCAGCTGTACACATTACCTAACAAGTC6230.1263635294165372No Hit
GATAGTATCAAAGTAGTATTCTAAGGTTACATGTTGTAAGATGTATTGCA6220.12616069871121371No Hit
TGCTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAATAAAGC6180.12534937588991973No Hit
CTATAATAAGATAGTGGTAGTAGGATTAGAGAAAGTGAATAGAATGCAAA6160.12494371447927272No Hit
CTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATACATG6140.12453805306862574No Hit
GTTATTTTGTTTGTTTGTTTGTTTTTGTTAGTAGCCATCCTGATGAGTGT6100.12372673024733177No Hit
TTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTTTTCAA6040.12250974601539079No Hit
GGTAAGTACTTAACACAGTACAATCTAGGCTGGCTCATCGACTTAATAAC6030.1223069153100673No Hit
CCGTATGATCCAGCAAATGCACTTCTGGGTGTTATTTCCAAAGGAATTAA6010.1219012538994203No Hit
GTATTGCAGTGGAAAAGAAGTTGAGTTATTAAGTCGATGAGCCAGCCTAG5920.12007577755150886No Hit
AAATAAAGCAGACACAAAAGGACAAATAATACATAATTCCACTTACAAGA5920.12007577755150886No Hit
CTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAATAAAGCAG5870.11906162402489139No Hit
TCTGTAGAGTGCTCTTCCCCTTTCCTAAGCACATTCCAGTTGGGCTTTGA5860.1188587933195679No Hit
GTTACATGTTGTAAGATGTATTGCAGTGGAAAAGAAGTTGAGTTATTAAG5860.1188587933195679No Hit
GCAATACATCTTACAACATGTAACCTTAGAATACTACTTTGATACTATCA5850.1186559626142444No Hit
ATCATACGGTAATCCGTTTTTAATTTTTAATTTTTTGATGAACTGCCATA5840.1184531319089209No Hit
TTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAAG5770.11703331697165643No Hit
GGTCTGAGCGAAGCACAGTTCAGTTTTGTTGAAAGCAACTCAGACAGTGA5750.11662765556100946No Hit
GTCCTATGTGACTCCATTGGAAAAGGACTCTGGAGTCTCTGACTGGTTTC5690.11541067132906847No Hit
CACAATGGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTC5630.11419368709712752No Hit
ATCTAGGCTGGCTCATCGACTTAATAACTCAACTTCTTTTCCACTGCAAT5600.11358519498115703No Hit
ACATAATTCCACTTACAAGAGATGCATAAAGAAACTGGACTGGATTGCCA5560.11277387215986305No Hit
GTATAGTGTGTGTCTACTTCCAAAGTCAAGTCTGAAACTAAAAATCAGAG5550.11257104145453954No Hit
ATGCTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAATAAAG5550.11257104145453954No Hit
GCCATGAACTGTACACTTAAAGATGATTACACTGGCAAATTCTGTTACAT5550.11257104145453954No Hit
CTTCTGTAGAGTGCTCTTCCCCTTTCCTAAGCACATTCCAGTTGGGCTTT5530.11216538004389257No Hit
GCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCAAAATTATTTCTC5530.11216538004389257No Hit
AGTCCATTGTGTGTCTTTATCAACATTTTGTTCACGTGTTCACCTGCCAG5520.11196254933856908No Hit
TGCCAGGACTGTTATTTTGTTTGTTTGTTTGTTTTTGTTAGTAGCCATCC5490.11135405722259858No Hit
GCCAGGGACTGAGGGGAAGGAGGGCTGGCAAGATGTGTTTAATGTGTATA5420.10993424228533412No Hit
TTATTTCTCCTTTCAAAATTCACTGCCCCTTCTAATTTACCAGTTTCTGT5410.10973141158001064No Hit
ATCTTGGAGACTTACACAGAGGGCATTAGAGCCTTATCATCACTTCAAAC5400.10952858087468714No Hit
GTGTTTAATGTGTATAGAGTTTGGAAGAGCAAGAAAGTTCTGCAGATGGA5390.10932575016936363No Hit
GGTCCTATGTGACTCCATTGGAAAAGGACTCTGGAGTCTCTGACTGGTTT5340.10831159664274617No Hit
TCCTAGGGCTGTACCTTCAGTACAGAGTCAGATTAGGGTCCCTTCTCACT5340.10831159664274617No Hit
GATACATGCTACAACATGCATGAATCTTGAAAACATGACACTAAATAAAA5330.10810876593742268No Hit
ATTTGAGCCCCTGCTTTTAATTCCTTTGGAAATAACACCCAGAAGTGCAT5320.10790593523209917No Hit
CTACAGAAGAACAGAGAAATGGATACTAGGACAGGACAAAGCAGATTCTT5280.10709461241080519No Hit
CTCTATACACATTAAACACATCTTGCCAGCCCTCCTTCCCCTCAGTCCCT5280.10709461241080519No Hit
TCCCTGGATTTTGTTTTTAGAATATGGGAAGATCTTGACTATTTCAGCAT5260.10668895100015821No Hit
GTTAAGTAATAGTCCATTGTGTGTCTTTATCAACATTTTGTTCACGTGTT5240.1062832895895112No Hit
AATAGTGAGAGTTTGTGAAAGAGCAGTTACTAGGACTTGGCAACTGGACC5230.10608045888418773No Hit
GAATGAAGATGAAGCAGTGAGAAGGGACCCTAATCTGACTCTGTACTGAA5230.10608045888418773No Hit
CCACTGAGCTGCATCACTAACTAACGAAAGAAGACTGCAGGAGGAACAGG5200.10547196676821723No Hit
ATTACTTAACCTTAAAAAGGAATGAAGTTCTAATAGTTCTGATACATGCT5200.10547196676821723No Hit
GTTGTAGCATGTATCAGAACTATTAGAACTTCATTCCTTTTTAAGGTTAA5180.10506630535757025No Hit
ATTAGAAGGGGCAGTGAATTTTGAAAGGAGAAATAATTTTGGATTTTGGA5150.10445781324159976No Hit
GTTACTAGGACTTGGCAACTGGACCTAATAGCTAAGAGAAAGTGAGAAGG5150.10445781324159976No Hit
AAGACACACAATGGACTATTACTTAACCTTAAAAAGGAATGAAGTTCTAA5100.1034436597149823No Hit
GGGTAACTATTAGAACTGGGGTTTTATTGACAGAAACTGGTAAATTAGAA5090.1032408290096588No Hit
GTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTCATGTTTTCAAGATTCA5060.10263233689368832No Hit
GAGGGCATTAGAGCCTTATCATCACTTCAAACTAATCCTTCCAAAATCCA5040.10222667548304132No Hit
TCCCAGCACCCTGGGATCATGACCTGAGCTGAAGGCGGACTTCACTGACT4980.10100969125110036No Hit
GCCTATAAATGCTACTATATTAGGTAAGTACTTAACACAGTACAATCTAG4970.10080686054577685No Hit
AACTAATCCTTCCAAAATCCAAAATTATTTCTCCTTTCAAAATTCACTGC4970.10080686054577685No Hit
ATCTTTAAGTGTACAGTTCATGGCATTGTTGTGGACCATCGCCATCATCC4970.10080686054577685No Hit
GAATTATGTATTATTTGTCCTTTTGTGTCTGCTTTATTTTATTTAGTGTC4960.10060402984045337No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)