FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C9.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C9.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5378166
Sequences flagged as poor quality0
Sequence length151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGG265840.4942948953230525No Hit
ATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTTGAA220410.4098237205768658No Hit
CCTCTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTC192390.3577241758621805No Hit
GATCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTG191070.3552698075886836No Hit
GTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCA179660.33405439698216827No Hit
GTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTT164860.3065357224005358No Hit
GTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAA144390.2684744204622914No Hit
CTGTACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACA136480.25376680452035133No Hit
GTCTTAACATGCGTGCTGTGAGTCCTCCGCAAAGTAGGGACAGTAATACG128780.23944965625828582No Hit
GTCCGAAGACCTACCAGAGAACATGCAGTTCAACAGGAATGAAAAGAAAA127180.23647466441162282No Hit
TGTTAAGACACTTTCTTTTGGTCATAGCTGGAGCCCATATTTGAAGCGGA125850.23400170243908425No Hit
GGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAAGATTTGT123830.23024577523267226No Hit
GTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAG114300.21252598004598594No Hit
ATCCAAGCTATCCCCTCAGTGAAACAAATGCTTGAGAAAGAAGATTTTTA110110.20473522014753728No Hit
TGTCTGGACTGAGGAAAATCCCTCTGGTACCCACTGTCCTTTCCTCTATG107540.19995663949383488No Hit
GTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAGAG107320.19954757811491874No Hit
GTCCAGACACCTGAGTCCAGTCCCTGGGGACTTCAGGCTGGGTTATTTTG107290.19949179701779382No Hit
GAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACAGACAC106750.19848773726954505No Hit
TCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTCCAAGATTAGACTAAGGA104420.1941554053928421No Hit
TCGTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGA103430.19231462918771938No Hit
GCTCTAAGTGGATGTCACTGAAGTCCTGTAGCCAGGAGCTATTCGTATTA103300.19207291110017802No Hit
CCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAGCTATGACCAAAA100240.18638323919343508No Hit
ACTTAGAGCACTGCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTT98010.18223684430714857No Hit
GCCTTTTATGGGAGGGATTTTTTTTAAAAACAAACTCCACCAGCTCAGGT97460.18121419085985818No Hit
GAGTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTG95990.17848091710073657No Hit
GCCTGGCACCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAG94260.17526420716653224No Hit
GAATATGGGAGGCGAAGGTTGCAGTGAGCCAAGATCGCACCACTGTACTA94120.17500389537994923No Hit
GAGTATATCCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTA90510.16829157002591588No Hit
GGATAGTGGTAAGGGGAGTGAAGGGAAGAGAGAAATTCAGTTTCCTGGAA89850.16706438588916742No Hit
CCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGCTCCTC88610.16475876720800361No Hit
GGTTTAGGGGCCGTACTCAGCTGGTTGAGTGTCATAGAGGAAAGGACAGT88380.1643311121300458No Hit
GGTACCCACTGTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCC80860.15034865045072987No Hit
GTCTCAACCTGAGCTGGTGGAGTTTGTTTTTAAAAAAAATCCCTCCCATA80840.15031146305264656No Hit
GTCCTTTCCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGC80740.15012552606223012No Hit
ATAAAAGGCTGGGCAGAGGGGCGTTACACCTGAGGGAATTTTCCAGATTA78150.14530975801044446No Hit
GTATTACTGTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACA76140.14157242450307408No Hit
GCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCCTCCGTGGGTATA75360.1401221159778259No Hit
GCTGAGTACGGCCCCTAAACCAGCTCATCCGCTTCAAATATGGGCTCCAG74650.1388019633458692No Hit
GCTCTGTGATGAGCCCTGAGATGTTTGAAGTGAGAACTCTCCAAACAGCC74360.13826274607366154No Hit
AGCTTGGATAGCCTCATTTTAGTTGATGTCACCATTTCAATCCCTACTTT73560.13677525015033004No Hit
CAGTAATACGAATAGCTCCTGGCTACAGGACTTCAGTGACATCCACTTAG73290.1362732202762057No Hit
AGATTAGACTAAGGACAAAATAACCCAGCCTGAAGTCCCCAGGGACTGGA73060.1358455651982479No Hit
AAATAACCCAGCCTGAAGTCCCCAGGGACTGGACTCAGGTGTCTGGACTG72770.13530634792604024No Hit
CCCTAGCTCCCTCATGCTCCACGACTTGGGGACAAGCTAGTTGTCTTCTC72300.13443244407108296No Hit
TCTCAGGGCTCATCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCT70820.13168057661291974No Hit
ACTACAGCCTGGGCAACGGAGCGAGACTCTGTCTCAAAAAAACACAAACA70500.13108557824358713No Hit
ATTCAGGAGACTGCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTAT68580.12751558802759155No Hit
GGAAAGGACAGTGGGTACCAGAGGGATTTTCCTCAGTCCAGACACCTGAG68400.12718090144484198No Hit
GCATGGAACATAGATTTAAATTCAGAAGCTGGGAGTATATCCTGAGGACA68190.12679043376496746No Hit
TCACAGAGCTTTCCATGCATGTTCCCAGGCAAGTCTCTTGTGGTCAGGGA67320.12517278194834447No Hit
GGTTGAGACAGGCAAATTTCTTTCTTATCTTTCTATGCCTAAAAATCTTC65480.12175154132468206No Hit
GTTCAACAGGAATGAAAAGAAAAACAAATGTAAAAGTCGTCTCCTCTGTA65110.12106357446014125No Hit
CCTGAGGACAGACACAAAGAAACCCCATGGTCCGAAGACCTACCAGAGAA63990.11898108016747716No Hit
AGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGC62960.11706592916618788No Hit
GTAGATGAAGTCTACACACAGAGCACCTGTGACCCATGTTCCTCCCTGCC62780.1167312425834383No Hit
CTACAGATCCTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGA61690.11470452938789914No Hit
GAGCACCTGTGACCCATGTTCCTCCCTGCCCCATCCGCAGGGGTGCCTGC61600.11453718609652436No Hit
GTCTTACTCTGTCACCCAGGCTGGAGTGCAGTGGCATGATCTTGGCTCAC61420.11420249951377477No Hit
TCTATACAGAGGAGACGACTTTTACATTTGTTTTTCTTTTCATTCCTGTT61280.11394218772719177No Hit
CTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAAGGTGCCAGGCAG60570.11262203509523508No Hit
GTTCGTTGAGATGGCATTTCCATGGAATTCAGGAGACTGCATGGAACATA60550.11258484769715178No Hit
CTTCCAAGGTGCCAGGCAGTGCTCTAAGTGGATGTCACTGAAGTCCTGTA59590.110799852589154No Hit
CTGTATAGATCTCTTAAGATGGAAGTGCCAGCAGCTACAGATCCTTCCAA58870.10946110625815565No Hit
GTCTTCGGACCATGGGGTTTCTTTGTGTCTGTCCTCAGGATATACTCCCA58230.10827110951949048No Hit
CCTCATAAGTATGTCCTGGAAGAGAAGGTAATGGTGAAATTTCTCCAACT58030.1078992355386576No Hit
CCCCAGGGACTGGACTCAGGTGTCTGGACTGAGGAAAATCCCTCTGGTAC57340.1066162703047842No Hit
GTCCCTACTTTGCGGAGGACTCACAGCACGCATGTTAAGACACTTTCTTT56530.10511018068241106No Hit
CCTCTATGACACTCAACCAGCTGAGTACGGCCCCTAAACCAGCTCATCCG56440.10494283739103628No Hit
CCTTGGAAGGATCTGTAGCTGCTGGCACTTCCATCTTAAGAGATCTATAC56200.10449658861403682No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA55460.1031206548849552No Hit
AGCCTGAAGTCCCCAGGGACTGGACTCAGGTGTCTGGACTGAGGAAAATC54910.10209800143766481No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA53790.10001550714500074No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)