FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C8a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C8a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences973720
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTAGAACTGAGAGCCACTTCTATCTAGAAGTCATTTTCTGTAACGAAC49880.5122622519820893No Hit
ATTTAAGAGTAACCATTTAACTACAAGATATGCAAAAACCTTCTGAAATA39090.40145010886086346No Hit
CCATTTAACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATG36040.37012693587478945No Hit
GCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATA32880.3376740746826603No Hit
AAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTA31880.32740418190034093No Hit
TTATAAAAGTGGCTATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTT30950.3178531816127839No Hit
GATTAGGACATTCCCCTGCTCCAAACCCTCCAGGGGCTCCCATTACAGTT28570.2934108367908639No Hit
CTTCTATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGC27830.2858111161319476No Hit
GTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCT26230.2693792876802366No Hit
ACTCAATACTCAGTAAGTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTT26070.2677361048350655No Hit
ATGTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTT25380.2606498788152652No Hit
GTGTCAGGACTTGGCAACCTCTTCTGTAAAGGACCGGATAGTAAATACTT24370.25027728710512265No Hit
CTTATGAGGATGTCAAAAATGCAGTTGGGGTCCTCATCGGAGGACTTGAA23820.24462884607484697No Hit
GTAGTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTA23580.24216407180709032No Hit
GTCCTGACACAGGGCACTGAGTTTTATTCTAACTGTAATGGGAGCCCCTG23200.23826151254980896No Hit
GTAGAAACAAGGGTGTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCT22590.23199687795259416No Hit
GTTATAAGGTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCAC22290.22891591011789839No Hit
CTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTGA20830.2139218666557121No Hit
GTCCAAGTCTGGCCCACCACTTGTTCTTGTAAACCAAGTTTTATTGGAAC20480.21032740418190035No Hit
GTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTT20220.20765723205849732No Hit
ATCTAGAAGTCATTTTCTGTAACGAACTAAAATTCCTCAGCTTGCATACT19590.20118719960563614No Hit
GTTCAGGGATGTTTGGAAAGCCGGCCCTGCCTTTGACGAGTTTGTTCCCT18550.190506511112024No Hit
CCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAACAGCAGAACCAAA18490.1898903175450848No Hit
CTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTACAAGATATGC17510.17982582261841185No Hit
CCTTATAACTTTCCTCAATTTAGGGAGTGGAGTTGATCCTCATCTTTGCC17450.1792096290514727No Hit
CCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAGA17230.17695025263936245No Hit
CCCCAGTGCCTGGGAAGTGGTTGCACGTGCTCTCCATGTTTGAGGAGTGA16800.17253419874296513No Hit
CATTTGGGTGGTACGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCT16600.17048022018650127No Hit
ACTCGGGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACT16520.1696586287639157No Hit
CGTTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGAT16380.168220843774391No Hit
CCCTAAATTGAGGAAAGTTATAAGGTGGAGAAGTGGGGAACGCAGTGAAC16190.16626956414575034No Hit
TCCCTGAACTTACTGAGTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGG15920.16349669309452408No Hit
GTATTGAGTTGGGGGCAGAGCCCAGTGGGAGAGGCGTCAAGTGCATCCCA15820.16246970381629217No Hit
GGGCAGAAGTGTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGAT15450.158669843486834No Hit
GCTCCAAACCCTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGC15440.1585671445590108No Hit
CTCCATGTTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAA15140.155486176724315No Hit
TTTGAGGAGTGAGTGAGTGAATAAACTAGCCATGTTCCATGAATTTCTCA14230.1461405742924044No Hit
GTTCTAAATTGGTAACAAGGCCGATTTCCACTAGGCATCAACTAGTTAAA14220.1460378753645812No Hit
GATTGAAAGCGACAGAGAATAGTTTCGAACAAATAACATTTATGCAAGCC14150.14531898286981884No Hit
TCCTTTAACTAGTTGATGCCTAGTGGAAATCGGCCTTGTTACCAATTTAG13600.1396705418395432No Hit
CTATAAAAGGCGAGACAATAAACTTTATCTAGAGACAAAACTAACAATAC13030.13381670295362116No Hit
GTTCTACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCG12950.1329951115310356No Hit
TTAGGAGTCTTTCCAAGTTCTTTTTTGTGTGTCCTTTAACTAGTTGATGC12850.1319681222528037No Hit
GGAGTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAG12840.1318654233249805No Hit
GCATAAATGTTATTTGTTCGAAACTATTCTCTGTCGCTTTCAATCTGTGC12680.1302222404798094No Hit
AGATAGAAGTGGCTCTCAGTTCTAAATTGGTAACAAGGCCGATTTCCACT12670.1301195415519862No Hit
CTCCAGGGGCTCCCATTACAGTTAGAATAAAACTCAGTGCCCTGTGTCAG12370.12703857371729038No Hit
ACTACAAGATATGCAAAAACCTTCTGAAATACTCGGGAGTATGCAAGCTG12280.12611428336688166No Hit
ACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGG12230.1256007887277657No Hit
GACCTGAGGATGACTCGGACGTCACCTCCCTCCTCTGAGCCGCAGTGGCC12220.1254980897999425No Hit
CCTCAAGGATGTGGGATGCACTTGACGCCTCTCCCACTGGGCTCTGCCCC12180.12508729408864971No Hit
GTATGCAAGCTGAGGAATTTTAGTTCGTTACAGAAAATGACTTCTAGATA12150.12477919730518013No Hit
GAACCAGAGACTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGA12100.12426570266606417No Hit
GTCCTAATCTAATTTATATTTTTAAAATACCCCCCTGGTGGAGGAAAGAC12040.12364950909912502No Hit
GAAATAAGGTGGTTAATTGAAGAAATGCGGCACAGATTGAAAGCGACAGA11650.11964425091402046No Hit
GTGCAGGAGGCAGCGGATCTGGCGGGACAGACCTGAGGATGACTCGGACG11590.11902805734708129No Hit
GTTTTTTATCATTAAATAAGCTGAAACGAGAAAGCTCTTATCTCTTGTTC11570.1188226594914349No Hit
CCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCC11570.1188226594914349No Hit
GTATTTACTATCCGGTCCTTTACAGAAGAGGTTGCCAAGTCCTGACACAG11550.11861726163578852No Hit
CTTCTGCCCACTTCTCACCACCTCTCCCGCAGCACCGCATCTCCCTGCAG11490.11800106806884936No Hit
CCATATGGCCCACTAAGTTGAAAGTATTTACTATCCGGTCCTTTACAGAA11480.11789836914102617No Hit
GTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACTATTCTCTGTC11380.11687137986279422No Hit
GGCTCACACAGAAACCTGTAGATAAGGGGGCAGCAAACCACAGGCTGCAG11310.11615248736803188No Hit
CTGCAGGATTATCTGTGGCTTTCAATGATCTGTGTTCCGAGGGTCTGATG11260.11563899272891591No Hit
GAATAGTTTCGAACAAATAACATTTATGCAAGCCTTACAACTACTGCTTG11240.11543359487326953No Hit
GTGGAGAAGTGGGGAACGCAGTGAACCAGAGAGGGAAGGCACCTCCGCCT11230.11533089594544634No Hit
CTTCTGTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATG11140.11440660559503758No Hit
GTAGATAAGGGGGCAGCAAACCACAGGCTGCAGTCCAAGTCTGGCCCACC11100.11399580988374482No Hit
GCCATGCCCACTCCCTTACAAATTGTCTGTGGCTGCTTTTGTGCTACAAC10870.11163373454381137No Hit
ACTTCAAGCAGTAGTTGTAAGGCTTGCATAAATGTTATTTGTTCGAAACT10800.110914842049049No Hit
GTAAAGGACCGGATAGTAAATACTTTCAACTTAGTGGGCCATATGGTCTC10730.11019594955428665No Hit
GTATTGTTAGTTTTGTCTCTAGATAAAGTTTATTGTCTCGCCTTTTATAG10550.10834736885346917No Hit
TTACAGAAGAGGTTGCCAAGTCCTGACACAGGGCACTGAGTTTTATTCTA10290.10567719673006615No Hit
ATGATGGACGACTAATCCAGAATAGCATAACAATAGAGAGGATGGTGCTT10250.10526640101877337No Hit
GAATAAAACTCAGTGCCCTGTGTCAGGACTTGGCAACCTCTTCTGTAAAG10130.10403401388489504No Hit
CCCCAAAGCCCTAAGAAGCCATGTGAAATTTAAGAGTAACCATTTAACTA10120.10393131495707185No Hit
GTGTTCCGAGGGTCTGATGTCAGCCATGGAGGATACAGCTGCTGCACCTT10090.10362321817360227No Hit
CTCCCGAGTATTTCAGAAGGTTTTTGCATATCTTGTAGTTAAATGGTTAC9990.10259622889537033No Hit
TGGTAACACGGTTCGAGTCTCTGAAAATATACAGAGATTCGCTTGGAGAA9960.10228813211190074No Hit
AGTTAAAGGACACACAAAAAAGAACTTGGAAAGACTCCTAACGTACCACC9780.10043955141108327No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)