FastQCFastQC Report
Mon 11 Dec 2023
H7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C7a.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameH7GWJBGXV_n01_CIVIR_Ferret_Flu_R2_C7a.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences300891
Sequences flagged as poor quality0
Sequence length151
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTTATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACA15960.5304246388226965No Hit
GGATACACCATGCAATCTATTCGAGAAATTTTTCCCTAGCAGTTCATATA10970.36458385262437226No Hit
TTCTTGATCCGTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCT9350.310743757706279No Hit
GTCCAGACTCGAAGTCGACCCTGGCATCAATCCGGGCCCTAGACACCATG8860.29445879072488046No Hit
CACTAAGACCACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAG8050.2675387432658338No Hit
GTTCATTGATGCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTG7890.2622212030270098No Hit
ACTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGA7580.25191846881428825No Hit
AATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCA7510.24959204495980272No Hit
CCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGTAT7490.24892735242994973No Hit
GTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAGTGCATGTGTG7110.2362981943627427No Hit
TTTTTAAACAATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCG6980.23197769291869813No Hit
GAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCACTCGCGAGATA6910.2296512690642126No Hit
AGTAGAAACAAGGCATTTTTTCATGAAGGACAAGTTAAATTCATTATTTT6710.2230043437656826No Hit
ATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGCCT6390.21236926328803452No Hit
CATAAATACGAGGCTTTTCATACGTAAAGTAAACGTCTTTATCGACGATC6310.20971049316862253No Hit
ATACTGGAGATCCTCCATACAGCCATGGAACAGGAACAGGATACACCATG6300.20937814690369602No Hit
AGCAGGTACTGATCCAAAATGGAAGACTTTGTGCGACAATGCTTCAATCC6110.20306356787009247No Hit
TCATATAGGAGACCGGTTGGAATTTCTAGCATGGTGGAGGCCATGGTGTC5740.19076675606781193No Hit
GCTTAATGCATGTCCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCAT5390.17913463679538438No Hit
GTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGAATCTAGAAAA5310.17647586667597234No Hit
GGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATG5230.17381709655656033No Hit
ATCTAATGTCGCAGTCCCGCACTCGCGAGATACTCACTAAGACCACTGTG5220.17348475029163385No Hit
CTATAAAGAAGGGATCGTCGATAAAGACGTTTACTTTACGTATGAAAAGC5200.17282005776178083No Hit
GGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCTATATGAACTGCTAG4890.1625173235490593No Hit
GGATAGACCCATTCAAATTACTCCAAAACAGCCAAGTGGTCAGCCTGATG4850.16118793848935328No Hit
GTATTAATGTAAACGTTTCTGTACGCTTATATGCAAACAACGACAACGTG4620.15354397439604375No Hit
ATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGATGCT4620.15354397439604375No Hit
TCAATGAACTGAGCAATCTTGCAAAAGGAGCTAATGTGCTAATTGGGCAA4430.14722939536244023No Hit
ATTCAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCC4420.14689704909751372No Hit
CTTCAATGGTGGAACAGATCTTCATGATCTCAGAGAACTCTTCTTTCTTG4380.14556766403780771No Hit
CTGTGGACCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAG4360.14490297150795473No Hit
TTTTTGGACAGTATGGATAGCAAATAGTAGCATTGCCACAACTACTTCAG4350.14457062524302822No Hit
CTATTAGAAGAGGCCTTATTTTCTCAATTTTCTTCTTTGTTGATTCATTG4260.1415795088586897No Hit
CCATATGGCCATAATCAAAAAGTACACATCAGGAAGGCAAGAGAAGAACC4240.14091481632883668No Hit
ACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGAGAGAAGAAATAAATA4160.13825604620942467No Hit
CCCATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTA4140.13759135367957168No Hit
ATTCGACACTAATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCT3940.13094442838104164No Hit
CCATTTGAATGGATGTCAATCCGACTCTACTTTTCCTAAAAATTCCAGCG3930.13061208211611514No Hit
GTATTTATGTGTGTAAATGAACGAGCGTACGTAGTTATCTCTGTTTTCTT3810.12662392693699712No Hit
CATCAGGAAGGCAAGAGAAGAACCCCGCACTCAGAATGAAGTGGATGATG3790.12595923440714413No Hit
GTCCCGCACTCGCGAGATACTCACTAAGACCACTGTGGACCATATGGCCA3780.12562688814221762No Hit
CCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCGAGA3760.12496219561236462No Hit
CCTCCATACAGCCATGGAACAGGAACAGGATACACCATGCAATCTATTCG3730.1239651568175851No Hit
TAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTACT3710.1233004642877321No Hit
GTTAAATTCATTATTTTTGCCGTCTGAGTTCTTCAATGGTGGAACAGATC3700.1229681180228056No Hit
TTGCAAGATTGCTCAGTTCATTGATGCTTAATGCATGTCCATTATTCTCT3660.12163873296309959No Hit
TCCCAGGACTCAGTGATGCTGTGCCATCTATTAGAAGAGGCCTTATTTTC3650.12130638669817309No Hit
AATTGATGGCCATCCGAATTCTTTTGGTCGCTGTCTGGCTGTCAGTAAGT3640.12097404043324658No Hit
CATATAGACTGTTGAATACAGATTAAACTTCCAAATGTGTGCATATTGCA3620.1203093479033936No Hit
GGTCTATCCCGACCAGTGAGTACCCTTCCCTTTCAAAGTCATGCCCACTT3610.1199770016384671No Hit
GAGTAAGACCTGCTGTTGCATCTTCGCCATTGTTTGCTTGGCGCCAAACT3590.11931230910861407No Hit
TCTATATATGGGTCCTCCTGTTTTCTTAGGGTCCTTCCCAGCACTGGGAT3510.11665353898920208No Hit
AGCGAAAGCAGGTCAAATATATTCAATATGGAGAGAATAAAAGAACTGAG3500.11632119272427556No Hit
TCTATACACAGGTTGGATAGAAGAGCCTTTGTGGTCTAGAAAGGTTAGAT3460.11499180766456957No Hit
AATAAATACACATCGCTCTATAAAGAAGGGATCGTCGATAAAGACGTTTA3380.11233303754515755No Hit
GTATTCAACAGTCTATATGCGTCTCCACAACTTGAGGGGTTTTCGGCTGA3370.11200069128023105No Hit
ATATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGA3370.11200069128023105No Hit
CTCTTATTCAGTCTTTGTTTTTTCTTCCCTATTGTTCTTTGCGTGACCAT3370.11200069128023105No Hit
CTCCTATATGAACTGCTAGGGAAAAATTTCTCGAATAGATTGCATGGTGT3340.11100365248545153No Hit
GCCCTAGACACCATGGCCTCCACCATGCTAGAAATTCCAACCGGTCTCCT3310.11000661369067204No Hit
ATCCAGAATAGCATAACAATAGAGAGGATGGTGCTTTCTGCTTTTGATGA3270.10867722863096604No Hit
AAACAAGGTCGTTTTTAAACAATTCGACACTAATTGATGGCCATCCGAAT3240.10768018983618652No Hit
GAATGAAGTGGATGATGGCAATGAGATACCCAATTACAGCAGACAAGAGA3220.10701549730633353No Hit
ATATAGAAGAGTAGACGGAAAGTGGATGAGAGAACTCATCCTTTATGACA3200.10635080477648053No Hit
CACTACGTCCCCTTGCCCAATTAGCACATTAGCTCCTTTTGCAAGATTGC3190.10601845851155403No Hit
CAATATGGAGAGAATAAAAGAACTGAGAGATCTAATGTCGCAGTCCCGCA3130.10402438092199501No Hit
GGTGTATCCTGTTCCTGTTCCATGGCTGTATGGAGGATCTCCAGTATAAG3110.10335968839214202No Hit
CATTAGCTCCTTTTGCAAGATTGCTCAGTTCATTGATGCTTAATGCATGT3110.10335968839214202No Hit
ATATGAAGCAATCGAGGAGTGCCTGATTAATGATCCCTGGGTTTTGCTTA3070.102030303332436No Hit
ATCATACAGAAGTCTATTTCCATACAACCGATACGTATTTACCATACGCA3070.102030303332436No Hit
GCATTAAGCATCAATGAACTGAGCAATCTTGCAAAAGGAGCTAATGTGCT3060.10169795706750949No Hit
CCATTATTCTCTTGTCTGCTGTAATTGGGTATCTCATTGCCATCATCCAC3010.10003622574287697No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)